Micro Test 3 Practice Questions
in fermentation an end product cannot be
pyruvate because that is what is being oxidized
What is the end product of glycolysis? A: alcohol B: lactic acid C:pyruvic acid D: acetyl CoA
pyruvic acid
in the step of glycolysis NAD has undergone
reductions
staphylococcus Arueas is capable of growth on a nutrient agar containing 10% salt. this medium is described as a selective differential undefined chemically defined both selective and differential
selective and differential
Two cells with a generation time of 20 minutes grew over 2 hours to a colony of cells. How many cells were in the colony? A:32 cells B: 128 cells C: 256 cells D: 64 cells
B
the agar is added to the culture media to serve as a solidifying agent reducing agent carbohydrate agent protein source growth factor
solidifying agent
in the reaction shown below ATP is formed via A: Chemiosmosis B: Photophosphorylation C: substrate phosphorylation D: oxidation- reduction
substrate phosphorylation
in the diagram shown below the pink arrow represents A: activation energy with an enzyme B: activation energy without enzyme C: the energy level of the products
the activation energy with enzyme
where does the ATP get made in the mitochondrion?
the matrix
how are aerobic respiration and anaerobic respiration different? Anaerobic respiration a: doesnt use chemiosmosis b: uses an organic electron acceptor c: uses an inorganic electron acceptors such as nitrates or carbonate d: produces more ATP per glucose molecule than aerobic respiration
uses inorganic electron acceptors such as sulfate, nitrate, or carbonate
what does glycolysis produce
2 pyruvate 2 ATP 2 NADH
a culture is inoculated with 2 cells. How many cells are in the culture after 6 generations A:60 b:64 c:320 D:640
2^6=64
34 colonies grow on a pour plate made with 1 ml from the 3rd tube in a 1:10 dilution series. What is the population of the original sample 34 340 3400 24000
3400
these graphs indicate optimum enzyme activity will occur at
37: C and pH:5
Which of the following is most likely to be killed by atmospheric oxygen? A: obligate anaerobe B: aerotolerant anaerobe C: obligate aerobe D: facultative anaerobe
B: Obligate anaerobe
The most Carbon Dioxide is going to be produced in the Electron Transport Chain as a waste product of the breakdown of glucose? T/F
F
The main pathway(s) of cell respiration in metabolism is/are ____________________________. A: Glycolysis, Krebs (TCA) cycle, Electron Transport Chain (pentose phosphate pathway) B: glycolysis only C: Krebs (TCA) cycle and Electron transport chain (pentose phosphate pathway) D: glycolysis and the Krebs Cycle (TCA cycle)
Glycolysis, Krebs (TCA) cycle, Electron Transport Chain (pentose phosphate pathway)
Mr Big wanted to make wine for his upcoming festival but instead his wine ended up as vinegar. What happened to Big's wine? A: He fermented grapes but forgot to keep holes in the lid so the organisms would not die because they had enough air (oxygen). B: He fermented grapes but spoilage organisms turned wine into vinegar. C: He forgot to use grapes. D: He fermented grapes but yeast got into his wine and turned it into vinegar.
He fermented grapes but spoilage organisms turned wine into vinegar.
When a bacterial cell is placed in a solution with a higher solute concentration relative to it's cellular content, this would be an example of ____________________, and the cell would _______________________. A:hypertonic; plasmolyze B: hypertonic; lyse C: hypotonic; lyse D: hypotonic; plasmolyze
Hypertonic plasmolyze
Cells would be most sensitive to penicillin during which phase of growth? A: log phase B: stationary phase C: lag phase D: prolonged decline
Log phase
which of these is the oxidized form the electron carrier and thus has less energy A:NADPH B:NADH C:NAD+ D:O2
NAD+
which of these temperatures is most likely to kill a mesophilic bacteria below 0 c 15c 37c 45c above 60c
above 60c
which of these temperatures is where you would most likely to find hyperthermophilic prokaryotes below 0 c 15c 37c 65c above 90 c
above 90
the region of an enzymes that binds with substrate molecules is the A: coenzyme B: holoenzyme C: active site D: allosteric site e: Feedback site
active site
which of these terms describes microbes that do not use oxygen but can grow if it is present in small amounts A:obligate aerobe B: obligate anaerobe C:faculative anaerobe D: microaerophile E: aerotolerant anaerobes
aerotolerant anaerobe
the enzyme shown in the previous diagram is
all of the above
which of these is an endergonic reaction A: anabolic B: are also biosynthetic reactions C: breakdown of polymers into monomers D:catabolic E: A and B
anabolic
which enzyme converts hydrogen peroxide to water and oxygen peroxidase catalase superoxide dismutase all of the above
catalase
sugars and salts are used to preserve foods because they induce the formation of oxygen free radicals inhibit cell wall biosynthesis cause cell lysis by osmotic shock cause plasmolysis of cells change the ph of the food
cause plasmolysis of cells
the nutrient broth that grows most microorganisms is commonly used in microbiology labs and is an example of selective media enrichment media complex media chemically defined media differential media
complex media
the type of media that promotes the growth of fastidious bacteria is an example of selective media enrichment media complex media chemically defined media differential media
complex media
ATP gets made in the
cytosol of the prokaryotes
blood agar is an example of ______media A: enrichment B: selective C: chemically defined D: differential E: A and D
differential
the advantage of direct counting method over the serial plate method is that direct counting counts both live and dead cells counts only living cells does not not require incubation can be used with low numbers of cells
does not require incubation
which of these terms describes microbes that prefer oxygen but can grow if it is not present. They can grow with or without oxygen A: Obligate aerobe b: Obligate anaerobe c: facultative anaerobe D: microaerophiles E:aerotolerant anaerobe
facultative anaerobe
most marine microorganisms are A: Hyperthermophiles B: Acidophiles C:Psychrophiles D:Facultative Halophiles E: Obligate halophiles
facultative halophiles
the name of the first pathway is
glycolysis
trace elements include elements such as zinc iron and copper are used as enzyme cofactors are frequently added in high concentration in culture media A and B All of the above
include elements such as iron, copper and zinc and cofactors
in the previous slide the pathway labeled #2 is the
kreb cycle
Which growth phase is also called the exponential growth phase A: lAG b: Log c: stationary d:decline phase
log phase
which of these terms describe a microorganism that grows at human body temp of 37c A:Thermophile B: Psychophile C:Psychtroph D: Mesophile E:Hyperthermophile
mesophile
in eukaryotes the electron transport chains is located in the
mitochochorion
organisms such as Clostridium lack oxygen detoxifying enzymes thus they are obligate aerobes obligate anaerobes faculattive anaerobes microaerophiles aerotolerant anaerobes
obligate anaerobe
Which of these give the cells the ability to produce the most ATP? A: substrate level phosphorylation B: reductive phosphorylation C: photophosphorylation D: oxidative phosphorylation
oxidative phosphorylation
all bacteria require the following for growth except oxygen nitrogen phosphorus sulfur carbon
oxygen
which of the following are direct measurements of bacterial population
petrof hauser
A moleculeof ADP that accepts a phosphate becomes __________________ A: carboxylated B: phosphorylated C: oxidized D:reduced
phosphorylated
in prokaryotes in electron transport chain is located in the
plasma membrane
the pathway is shutdown by high concentrations of
product
Catabolic reactions A: provide building blocks for anabolism B: are also biosynthetic reactions C: Are endergonic D: include the formation of proteins from amino acids
provide the buliding blocks for anabolism
which of these describes a microorganism that grows in a refrigerator temperature and can cause food spoilage of 15c thermophile psychrophile psychrotroph mesophile hyperthermophile
psychrotroph