Microbiology Chapter 10 quiz

The experiment involves the transfer and expression of _______ DNA into bacterial cells

Frog

The resulting thousands of different size DNA fragments are then separated by size using

Gel electrophoresis

To carry out Sanger sequencing, a mixture is needed containing

All of these choices are correct. Single-stranded DNA, DNA polymerase, for deoxyribonucleotide's A T C G

Which of the following Escherichia coli cells did NOT produce colonies on the growth medium containing try tetracycline

Cells that did not pick up a plasmid

Human DNA cut with restriction enzyme A can be join to

Bacterial DNA cut worth restriction enzyme A

Correction or repair a faulty gene in human suffering from a fatal or debilitating disease is a process known as ______.

Gene therapy

The addition of radiolabeled probe molecules to the DNA fragments results in

Hybridization

Utilizing insulin made from recombinant DNA technology is beneficial because _______.

It will not cause an allergic reaction

_______ are excellent cloning vectors because they are small, well characterized, easy to manipulate, and can be transferred into appropriate host cells through transformation.

Plasmids

Which of the following were required to produce the recombinant plasmid?

Restriction endonuclease and DNA ligase

In traditional DNA fingerprinting, the DNA that has been collected is cut into smaller pieces by

Restriction enzymes

DNA micro arrays contain _______.

Single-stranded DNA

Please choose the statement the best expresses the goal of southern blot analysis.

Southern blot analysis is used to identify DNA fragments that are caring specific DNA sequences

Video. Which of the following could not be the recognition site of a restriction endonuclease

gcttgc cgaacg

Which of the following techniques utilizes an electric current and the fact that DNA molecules are negatively charged.

gel electrophoresis

When a dideoxyribonucleotide is added to the tube

replication of the strand stops

The primer used in Sanger sequencing

has a nucleotide sequence complementary to the 3' end of the region to be copied

DNA probes are used to

locate a specific DNA nucleotide sequence in a test sample

Video. The single-stranded ends of DNA molecules can be join together by

DNA ligase

For DNA probes to hybridize

DNA strands must be homologous, having regions with similar or identical nucleotide sequences

In the micro array procedure, white molecule is labeled with a fluorescent tag?

cDNA