Microbiology Chapter 10 quiz
The experiment involves the transfer and expression of _______ DNA into bacterial cells
Frog
The resulting thousands of different size DNA fragments are then separated by size using
Gel electrophoresis
To carry out Sanger sequencing, a mixture is needed containing
All of these choices are correct. Single-stranded DNA, DNA polymerase, for deoxyribonucleotide's A T C G
Which of the following Escherichia coli cells did NOT produce colonies on the growth medium containing try tetracycline
Cells that did not pick up a plasmid
Human DNA cut with restriction enzyme A can be join to
Bacterial DNA cut worth restriction enzyme A
Correction or repair a faulty gene in human suffering from a fatal or debilitating disease is a process known as ______.
Gene therapy
The addition of radiolabeled probe molecules to the DNA fragments results in
Hybridization
Utilizing insulin made from recombinant DNA technology is beneficial because _______.
It will not cause an allergic reaction
_______ are excellent cloning vectors because they are small, well characterized, easy to manipulate, and can be transferred into appropriate host cells through transformation.
Plasmids
Which of the following were required to produce the recombinant plasmid?
Restriction endonuclease and DNA ligase
In traditional DNA fingerprinting, the DNA that has been collected is cut into smaller pieces by
Restriction enzymes
DNA micro arrays contain _______.
Single-stranded DNA
Please choose the statement the best expresses the goal of southern blot analysis.
Southern blot analysis is used to identify DNA fragments that are caring specific DNA sequences
Video. Which of the following could not be the recognition site of a restriction endonuclease
gcttgc cgaacg
Which of the following techniques utilizes an electric current and the fact that DNA molecules are negatively charged.
gel electrophoresis
When a dideoxyribonucleotide is added to the tube
replication of the strand stops
The primer used in Sanger sequencing
has a nucleotide sequence complementary to the 3' end of the region to be copied
DNA probes are used to
locate a specific DNA nucleotide sequence in a test sample
Video. The single-stranded ends of DNA molecules can be join together by
DNA ligase
For DNA probes to hybridize
DNA strands must be homologous, having regions with similar or identical nucleotide sequences
In the micro array procedure, white molecule is labeled with a fluorescent tag?
cDNA