Microbiology Lab [ LAB EXAM 1 ] Review

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At the end of Gram staining, Gram positive bacteria stain purple while Gram negative bacteria stain red.

TRUE

Presence of flagella contributes to bacterial pathogenecity.

TRUE

turns red in reduced form

Tritetrazolium chloride

What is the purpose of using decolorizing agent in Gram staining?

a. In Gram negative cells alcohol increases the porosity of cell wall by dissolving the lipid in outer layer, removing the CV-I complexes b. In Gram positive cells alcohol dehydrates cell wall and shrinks the pores, making it difficult to remove CV-I complexes. >> both a and b are correct <<

In staining procedure used to identify Mycobacterium smegmatis, the decolorizer used is _______

acid alcohol

Negatively charged chromatogens

acidic stains

Primary stain in Acid-fast staining

carbol fuchsin

A streptococcus arrangement shows __________

cocci in chains

Use of two dyes

differential staining

depression slide

hanging drop technique

study of cell shape and arrangement is called ________

morphology

brownian movement

non motile bacteria

group of cocci arranged in cluster of 8

sarcinae

Use of a single stain

simple staining

irregular group of round bacteria with no specific arrangement

staphylococcus

a chain of round bacteria

streptococcus

If a bacterial cluster appears as a square of four cocci, this arrangement is called as _________

tetrad

diplobaccilus

two rod shaped bacteria attached together

What is the concentration of agar in the motility test media?

0.3%-0.5%

concentration of agar

0.3%-0.5%

Why are bacteria not stained in negative staining?

Both the bacterial cell wall and the dye are negatively charged, so they repel each other

Primary stain in Gram staining

Crystal violet

Differential staining

Decolorizing agent

All bacteria have flagella

FALSE

Amphitrichous flagellar arrangement show presence of a cluster of flagella at one end only

FALSE

The proper procedure for smear preparation is:

Make smear-->air dry-->heat fix

Counter stain in Acid fast staining

Methylene blue

Basic stains with a positively charged chromatogen

Methylene blue and Crystal violet

Acidic stain

Nigrosin

Counter stain in Gram staining

Safranin


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