Microscopy

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Fluorescent labeling

Follows molecules moving through a cell

Electron Microscope

Examples of this microscope are SEM and TEMs. This type of microscope magnifies 100,000x to 250,000x. Advantages are its the best resolution, the highest magnification and it projects image on tv monitor or computer. Disadvantages include that the specimens are dead, it's very expensive and very large. It's used to view and study DNA, viruses, proteins and Amino acids.

Center, Edge

Place a small, flat object in the - of the microscope slide, then put the - of the coverslip one side of the object and gently lower it onto the slide so it covers the object is how to create a dry mount slide.

Embedding

Placing in liquid wax or plastic and allowing it to harden.

Light source (or mirror)

Produces light or reflects light up toward the eyepiece

High-Resolution video technology

Produces movies of cells as they grow, divide and develop.

Scanning Electron Microscope (SEM)

Scan surfaces of specimens and make 3-D images

Confocal light microscopy

Scans cells with laser beam; used to build 3-D images of cells and cell parts

Scanning Objective

Shortest objective (Red) with a lens that has a magnification of 4x. You should always start here

Sectioning

Slicing thinly, done by a microtome

Stage

Supports slides being observed

Arm

Supports the body tube

Base

Supports the microscope

Cells, Basic Unit, pre-existing

The cell theory states that all living things are made of -, cells are the -- of structure function in living things and new cells are made of -- cells.

Transmission Electron Microscope (TEM)

Used to study cell structures and protein molecules

Field of View

Area that are seen when looking through a microscope. Commonly drawn as a circle when sketching images in a lab

Diaphragm

Controls the amount of light passing into the eyepiece

Fixation

Done by cutting the material into small pieces and allowing it to soak in a fixative, such as formalin.

Staining

Dying a specimen so that the structural details are easier to see.

Rotating nosepiece

Holds objective lenses and rotates to change magnification.

Stage clips

Holds the slide in place

Resolution

How clear an object appears. Clarity.

High Power Objective

Largest objective(blue) with a lens that has a magnification of 40x. Used last.

Light system

Light and diaphragm of microscope

Illuminator

Light source of a microscope

Stereoscope

Magnifies 10x-30x. Advantages include it's very inexpensive, the image is 3D and it doesn't flip the image. Disadvantages include its low resolution, low magnification and is for external examination only. It's used to view the external anatomy of specimens, insects, hydra, jellyfish and plants.

Compound Light Microscope

Magnifies from 40x to 400x. Advantages are that it can view living specimen, it's inexpensive, has good resolution and it has good magnification. Disadvantages include that specimens must be thin so light can pass through and it flips the image upside down and backwards. It's used to view pond water, unicellular organisms like paramecium, euglena and amoeba.

Body tube

Maintains proper distance between eyepiece and the objective lenses

Mechanical System

Major parts of a microscope

Low power objective

Medium objective(yellow) with a lens that has a magnification of 10x. Use second

Fine adjustment Knob

Moves body tube slightly to sharpen the image(high power)

Coarse adjustment Knob

Moves the body tube greatly when first focusing an image

Total magnification

Multiplying the magnifying power of the objective by the magnifying power of the ocular lens.

Ocular(Eyepiece)

Part one looks through. Contains magnifying lens(10x)

Magnification

The enlargement of an image

Magnifying Power

The extent to when a microscope magnifies images expressed as a number and followed by a multiplication sign. Eg. 10x

One drop, 45

To create a wet mount slide, place -- of water in the center of the slide, place the object being viewed I'm the center of the drop and touch the edge of the coverslip to the surface of the slide at a --° angle.


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