Microscopy
Fluorescent labeling
Follows molecules moving through a cell
Electron Microscope
Examples of this microscope are SEM and TEMs. This type of microscope magnifies 100,000x to 250,000x. Advantages are its the best resolution, the highest magnification and it projects image on tv monitor or computer. Disadvantages include that the specimens are dead, it's very expensive and very large. It's used to view and study DNA, viruses, proteins and Amino acids.
Center, Edge
Place a small, flat object in the - of the microscope slide, then put the - of the coverslip one side of the object and gently lower it onto the slide so it covers the object is how to create a dry mount slide.
Embedding
Placing in liquid wax or plastic and allowing it to harden.
Light source (or mirror)
Produces light or reflects light up toward the eyepiece
High-Resolution video technology
Produces movies of cells as they grow, divide and develop.
Scanning Electron Microscope (SEM)
Scan surfaces of specimens and make 3-D images
Confocal light microscopy
Scans cells with laser beam; used to build 3-D images of cells and cell parts
Scanning Objective
Shortest objective (Red) with a lens that has a magnification of 4x. You should always start here
Sectioning
Slicing thinly, done by a microtome
Stage
Supports slides being observed
Arm
Supports the body tube
Base
Supports the microscope
Cells, Basic Unit, pre-existing
The cell theory states that all living things are made of -, cells are the -- of structure function in living things and new cells are made of -- cells.
Transmission Electron Microscope (TEM)
Used to study cell structures and protein molecules
Field of View
Area that are seen when looking through a microscope. Commonly drawn as a circle when sketching images in a lab
Diaphragm
Controls the amount of light passing into the eyepiece
Fixation
Done by cutting the material into small pieces and allowing it to soak in a fixative, such as formalin.
Staining
Dying a specimen so that the structural details are easier to see.
Rotating nosepiece
Holds objective lenses and rotates to change magnification.
Stage clips
Holds the slide in place
Resolution
How clear an object appears. Clarity.
High Power Objective
Largest objective(blue) with a lens that has a magnification of 40x. Used last.
Light system
Light and diaphragm of microscope
Illuminator
Light source of a microscope
Stereoscope
Magnifies 10x-30x. Advantages include it's very inexpensive, the image is 3D and it doesn't flip the image. Disadvantages include its low resolution, low magnification and is for external examination only. It's used to view the external anatomy of specimens, insects, hydra, jellyfish and plants.
Compound Light Microscope
Magnifies from 40x to 400x. Advantages are that it can view living specimen, it's inexpensive, has good resolution and it has good magnification. Disadvantages include that specimens must be thin so light can pass through and it flips the image upside down and backwards. It's used to view pond water, unicellular organisms like paramecium, euglena and amoeba.
Body tube
Maintains proper distance between eyepiece and the objective lenses
Mechanical System
Major parts of a microscope
Low power objective
Medium objective(yellow) with a lens that has a magnification of 10x. Use second
Fine adjustment Knob
Moves body tube slightly to sharpen the image(high power)
Coarse adjustment Knob
Moves the body tube greatly when first focusing an image
Total magnification
Multiplying the magnifying power of the objective by the magnifying power of the ocular lens.
Ocular(Eyepiece)
Part one looks through. Contains magnifying lens(10x)
Magnification
The enlargement of an image
Magnifying Power
The extent to when a microscope magnifies images expressed as a number and followed by a multiplication sign. Eg. 10x
One drop, 45
To create a wet mount slide, place -- of water in the center of the slide, place the object being viewed I'm the center of the drop and touch the edge of the coverslip to the surface of the slide at a --° angle.
