MLBT1080 Unit Five Study Questions Recall Practice 1 Vibrio_ NonFermenters_ Anaerobes

How long are anaerobic cultures routinely held in the laboratory? A. 1 to 2 days B. 3 to 4 days C. 5 to 7 days D. 7 to 9 days

C. 5 to 7 days

What is an obligate anaerobe? A. An organism that requires some oxygen but moderate concentrations of CO2 to live B. An organism that requires small amounts of oxygen and carbon dioxide to live C. An organism that grows only in the absence of molecular oxygen D. An organism that can live with very little oxygen

C. An organism that grows only in the absence of molecular oxygen

What organism mostly commonly causes gas gangrene? A. Clostridium difficile B. C. tetani C. C. perfringens D. C. botulinum

C. C. perfringens

A microbiologist is working the miscellaneous bench reading stool cultures. The campy plate from a culture has growth on the plate that was incubated at 42degrees C. The colonies are nonhemolytic, moist, "runny looking," and spreading. The Gram stain shows tiny gram-negative rods with some S-shapes and seagull-wing shapes. What is growing on the plate? A. Aeromonas B. Helicobacter C. Campylobacter D. Acinetobacter

C. Campylobacter

What organism may play a role in Guillain-Barré syndrome (GBS)? A. Helicobacter B. Aeromonas C. Campylobacter D. Plesiomonas

C. Campylobacter

What disease does Clostridium perfringens cause? A. Botulism B. Tetanus C. Food poisoning D. Food poisoning Toxic shock syndrome

C. Food poisoning

Infections with Vibrio spp. can be contracted in all of the following situations EXCEPT: A. Increased travel to either coastal or cholera-endemic areas B. Increased consumption of seafood (particularly uncooked) C. Greater numbers of Vibrio spp. in the environment D. Increased use of recreational water facilities

C. Greater numbers of Vibrio spp. in the environment

All of the following are characteristics of nonfermenters EXCEPT: A. Thin gram-negative bacilli or coccobacilli B. Oxidase positive C. Indole positive D. Resistance to a variety of antimicrobials, such as aminoglycosides, third-generation cephalosporins, penicillins, and fluoroquinolones

C. Indole positive

All of the following procedures should be performed on clinical specimens to recover anaerobic bacteria EXCEPT: A. Specimen plating on appropriate tubed and plated media B. Anaerobic incubation C. Kinyoun stain D. Gram stain

C. Kinyoun stain

What is unique about the Sphingobacterium spp.? A. Sphingomyelin in the cell wall B. Cholesterol in the cell wall C. Sphingophospholipids in the cell wall D. Collagen in the cell wall

C. Sphingophospholipids in the cell wall

What disease does Clostridium tetani cause? A. Botulism B. Tetanus C. Food poisoning D. Myonecrosis

B. Tetanus

How is Helicobacter pylori presumptively identified? A. A culture of a gastric biopsy B. A culture of gastric scrapings C. A rapid oxidase test done on gastric scrapings D. A rapid urease test done on a gastric biopsy

D. A rapid urease test done on a gastric biopsy

What evidence indicates the presence of anaerobes in cultures? A. A foul odor upon opening an anaerobic jar or bag B. Growth on the anaerobic plates, but not on the sheep blood agar (SBA) plates incubated in the CO2 incubator C. Colonies on kanamycin-vancomycin laked blood agar (KVLBA) that fluoresce brick-red under ultraviolet light D. All of the above

D. All of the above

Which of the following is a virulence factor of Pseudomonas aeruginosa? A. Endotoxin B. Exotoxins C. Capsule D. All of the above

D. All of the above

How are suspected colonies of anaerobes processed? A. Gram stain morphology and reaction is observed. B. An aerotolerance test is set up. C. A pure culture/subculture plate is inoculated and appropriate disks are added. D. All of the above.

D. All of the above.

What organism is associated with pneumonia in patients with cystic fibrosis or chronic granulomatous disease (CGD)? A. Stenotrophomonas maltophilia B. Pseudomonas putida C. Pseudomonas fluorescens D. Burkholderia cepacia

D. Burkholderia cepacia

When collecting specimens that may contain Campylobacter spp. bacteria, what transport medium should they be placed in, if a delay in transport is possible? A. Bordet-Gengou B. Regan-Lewis C. Aimes D. Cary-Blair

D. Cary-Blair

All of the following are non-spore-forming, anaerobic, gram-positive bacilli EXCEPT: A. Actinomyces B. Bifidobacterium C. Eubacterium D. Clostridium

D. Clostridium

All of the following organisms are found on the skin EXCEPT: A. Propionibacterium B. Peptostreptococcus C. Eubacterium D. Clostridium

D. Clostridium

An elderly patient in a nursing home is recovering from bacterial pneumonia. The patient has been on a lengthy regiment of antibiotics to kill the organism causing the disease. A few days later, the patient is diagnosed with pseudomembranous colitis. What organism is the most likely cause? A. Clostridium difficile B. C. tetani C. C. perfringens D. C. botulinum

A. Clostridium difficile

A microbiology technologist is reading an anaerobic culture and observes a double zone of hemolysis on an anaerobically incubated sheep blood agar plate. The Gram stain of that organism was a boxcar-shaped, gram-positive bacillus. What organism is this? A. Clostridium perfringens B. C. difficile C. C. ramosum D. C. clostridiiforme

A. Clostridium perfringens

The three species of nonfermenters that make up the majority of isolates routinely seen in clinical laboratories include all the following EXCEPT: A. Pseudomonas aeruginosa B. Acinetobacter spp. C. Stenotrophomonas maltophilia D. P. putida

D. P. putida

Anaerobes outnumber aerobes in all of the following locations EXCEPT: A. Skin B. Oral cavity C. Gastrointestinal (GI) tract D. Genitourinary tract

A. Skin

An immunosuppressed patient notices sinus tracts that are draining pus. He also notices that there appear to be small, hard "nuggets" in the pus. What disease will his doctor most likely diagnose? A. Gas gangrene B. Pseudomembranous colitis C. Actinomycosis D. Myonecrosis

c. Actinomycosis

What species of Aeromonas is the most frequently associated with gastrointestinal infections? A. Aeromonas hydrophila B. A. caviae C. A. veronii D. A. sobria

A. Aeromonas hydrophila

Bacterial growth requirements for oxygen and carbon dioxide can be divided into all the following categories EXCEPT: A. Ambient air (95% O2 and 5% CO2) B. Microaerophilic (5% O2 ) C. Anaerobic (0% O2) D. Capnophilic (about 15% O2 and 5% to 10% CO2)

A. Ambient air (95% O2 and 5% CO2)

Which of the following is considered a better specimen for anaerobic culture than a swab? A. Aspirate B. Washing C. Scraping D. Lavage

A. Aspirate

What disease does Clostridium botulinum cause? A. Botulism B. Tetanus C. Food poisoning D. Myonecrosis

A. Botulism

What organism is the most common cause of bacterial gastroenteritis worldwide? A. Campylobacter jejuni B. C. fetus C. C. lari D. C. coli

A. Campylobacter jejuni

What disease manifests in acute cases as a severe gastroenteritis, accompanied by vomiting followed by diarrheic stools that are described as rice water and occur 10 to 30 times a day? A. Cholera B. Typhoid C. Dysentery D. Food poisoning

A. Cholera

What type of an atmosphere do campylobacters require for growth? A. Microaerophilic and capnophilic B. Anaerobic and moist C. Capnophilic and halophilic D. Microaerophilic and high nitrogen content

A. Microaerophilic and capnophilic

Despite all its virulence factors, what type of pathogen is Pseudomonas aeruginosa? A. Opportunistic B. Primary C. Secondary D. Commensal

A. Opportunistic

What biochemical test will differentiate nonfermenters from Enterobacteriaceae (except Plesiomonas)? A. Oxidase B. Indole C. Citrate D. Voges-Proskauer (VP)

A. Oxidase

What nonfermenter is the leading cause of nosocomial pneumonia and bacteremia? A. Pseudomonas aeruginosa B. P. fluorescens C. P. putida D. P. mendocina

A. Pseudomonas aeruginosa

Which of the following is a curved, gram-negative rod that grows on TCBS agar? A. Vibrio B. Campylobacter C. Clostridium D. Fusobacterium

A. Vibrio

What Vibrio species is most infrequently isolated in the laboratory? A. Vibrio alginolyticus B. V. parahaemolyticus C. V. cholerae D. V. vulnificus

A. Vibrio alginolyticus

What temperature is an optimal growth temperature for Campylobacter jejuni? A. 35 degrees C B. 42 degrees C C. 25 degrees C D. 60 degrees C

B. 42 degrees C

What is an anaerobe? A. An organism that can live with very little oxygen B. An organism that does not require oxygen to live C. An organism that requires some oxygen but moderate concentrations of CO2 to live D. An organism that requires small amounts of oxygen and carbon dioxide to live

B. An organism that does not require oxygen to live

A microbiology technologist is reading an anaerobic plate from an intestinal abscess. There is growth on the Bacteroides bile esculin (BBE) plate: gray colonies with a brown color in the area around the colonies. There is also a dark precipitate in the medium in the areas of heavy growth. The technician Gram stains the colonies and observes gram-negative coccobacilli. What is the presumptive identification of this organism? A. Bacteroides vulgatus B. B. fragilis C. B. pneumoniae D. B. denticola

B. B. fragilis

What organism has a microscopic morphology of tiny gram-negative rods, curved, with S-shapes or seagull-wing shapes on Gram stain? A. Helicobacter B. Campylobacter C. Haemophilus D. Acinetobacter

B. Campylobacter

What substances do nonfermenters fail to ferment? A. Lipids B. Carbohydrates C. Proteins D. Alkaloids

B. Carbohydrates

What organism is an opportunistic pathogen that strikes the immunocompromised patient with neutrophil deficits and produces a violet pigment on nonselective agar? A. Psychrobacter immobilis B. Chromobacterium violaceum C. Chryseomonas luteola D. Paracoccus phenylpyruvicus

B. Chromobacterium violaceum

What is the name of the organism that is strongly associated with gastric, peptic, and duodenal ulcers as well as GI carcinoma? A. Campylobacter jejuni B. Helicobacter pylori C. C. curvus D. H. rectus

B. Helicobacter pylori

A microbiologist is reading the plates from a sputum culture. On the sheep blood agar (SBA), she sees flat spreading colonies with a metallic sheen. On cetrimide agar, she sees a fluorescent green color in the media with clear colonies. On MacConkey, she sees medium clear colonies that have a fruity or grape-like odor. What is the most likely organism? A. Pseudomonas fluorescens B. P. aeruginosa C. P. putida D. P. stutzeri

B. P. aeruginosa

What organism causes pulmonary disease among individuals with cystic fibrosis? A. Pseudomonas fluorescens B. P. aeruginosa C. P. putida D. P. mendocina

B. P. aeruginosa

What type of bacteria are those in the genus Clostridium? A. Anaerobic cocci B. Spore-forming anaerobic bacilli C. Spore-forming anaerobic cocci D. Anaerobic bacilli

B. Spore-forming anaerobic bacilli

What is the name of the organism that causes the disease cholera? A. Vibrio furnissii B. V. cholerae C. V. vulnificus D. V. parahaemolyticus

B. V. cholerae

What are two important factors that must be taken into consideration when transporting specimens for anaerobic culture? A. Making sure there is enough specimen in a tightly closed container B. Minimum exposure to oxygen and drying out C. Minimum exposure to oxygen and making sure the specimen is transported in a tightly closed screw-cap container D. Drying out and proper nutrition

C. Minimum exposure to oxygen and making sure the specimen is transported in a tightly closed screw-cap container

In what single setting does Stenotrophomonas maltophilia produce all its disease? A. Community B. Military barracks C. Nosocomial D. College dorms

C. Nosocomial

All of the following characteristics describe the genus Plesiomonas EXCEPT: A. Ferments glucose B. Glucose positive C. Oxidase negative D. Facultative anaerobes

C. Oxidase negative

A patient goes to the dentist with an abscess. The physician cultures the tooth. Which of the following bacteria can possibly cause this infection? A. Bacteroides fragilis B. Porphyromonas asaccharolytica C. P. gingivalis D. Mobiluncus

C. P. gingivalis

All the following organisms are found in the gastrointestinal (GI) tract EXCEPT: A. Bacteroides B. Clostridium C. Propionibacterium D. Bifidobacterium

C. Propionibacterium

What disk is used to presumptively identify Peptostreptococcus anaerobius? A. Bile B. Nitrate C. Sodium polyanethol sulfonate D. Indole

C. Sodium polyanethol sulfonate

What is the name of the organism that is responsible for "summer diarrhea" in Japan? A. Vibrio cholerae B. V. vulnificus C. V. parahaemolyticus D. V. trota

C. V. parahaemolyticus

A patient is brought to the emergency department with a severe case of gastroenteritis. The patient experienced gastrointestinal upset after eating raw oysters. What organism can be the culprit of this patient's condition? A. Vibrio trota B. V. parahaemolyticus C. V. cholerae D. V. vulnificus

D. V. vulnificus

The four major groups of anaerobic gram-negative bacilli include all the following EXCEPT: A. Bacteroides fragilis B. Porphyromonas C. Prevotella D. Mobiluncus

D.Mobiluncus