Module 1 Post-Lab Quiz

Only laboratories BSL-3 and higher require fire extinguishers, emergency showers and eye wash stations. True False

All microbiology laboratories should be equipped with fire extinguishers, as well as emergency showers and eye wash stations.

What biosafety level is designated for organisms that don't usually cause disease in humans? BSL-3 BSL-5 BSL-1 BSL-2 BSL-4

BSL-1 is designated for organisms that do not usually cause disease in humans and require only a few specialized techniqutes.

Which of the following liquids can be safety pipetted by mouth? (Select all that apply)

None of the answer choices are correct. You should never pipette anything by mouth.

This image illustrates what correct safety practices? (Select all that apply) use of gloves and eye protection tying back of long hair uncluttered work bench wearing of the lab coat using a biohazard container autoclaving of infectious materials

In this image, the technician is wearing the correct personal protection equipment, and her lab bench is clean and free of unnecessary clutter

How long should hands be lathered while washing?

20 seconds

If gloves are worn in lab, hand washing is unnecesary.

False Hands should always be thoroughly washed after removing gloves.

When should soap be added to the hands?

after the hands are wet The soap will dissolve and lather better if it is added AFTER the hands are wet.

How many biosafety levels are there for selected infectious agents? One Two Three Four

there are four biosafety levels designed on the basis of microbes' ease of transmission and pathogenicity.

Which of these steps will prevent the immediate recontamination of the hands after washing?

using a paper towel to turn off the faucet handle

When should the chemical fume hood be used?

when using hazardous chemicals Chemical fume hoods do not provide protection from infectious microorganisms. If working with BSL-2 microorganisms, a biological safety cabinet should be used.

What biosafety levels do most introductory microbiology students work with?

BSL-1 Most introductory students work with BSL-1. Occasionally they may work with BSL-2 agents if appropriate safety measures can be applied. Any organism in the higher levels would require specialized equipment not usually found in these lab settings.

Identify which activity should be avoided prior to starting an experiment in the microbiology laboratory. Wearing open toed shoes. Wearing long hair pulled back. Wearing safety glasses. Wearing a lab coat.

Dressing appropriately for the lab is essential prior to starting an experiment in the microbiology laboratory. This includes avoiding clothes with baggy sleeves that could catch fire and wearing closed-toed shoes.

Which of the following are examples of safety equipment found in nearly every laboratory classroom? (Select all that apply) fire extinguisher first aid kit eye wasH chemical shower lead shielding centrifuges

Every laboratory classroom should be equipped with a fire extinguisher, first aid kit, chemical shower, and eye wash station. Many also have a chemical fume hood and some may have a biosafety cabinet.

Thoroughly washing hands will sterilize them.

False Washing hands will reduce the microbial burden and wil remove many transient microorganisms, but it will not sterilize the hands.

Goggles or appropriate safety glasses protect the eyes from (Select all that apply) chemical splashes chemical splashes fumes of preservatives used on anatomy specimens UV exposure when using UV radiation in the laboratory burns from the hot plate or Bunsen burner

Goggles protect they eyes from chemical splashes, aerosols, and UV exposure. They don't protect the eyes from fumes or hot plate/Bunsen burner burns.

Hand washing once just prior to labs is the only time hand washing is recommended to avoid personal contamination. True False

Hand washing before and after lab activities significantly reduces the risk of personal contamination.

Why is it recommended that hands be wet prior to adding soap? (Select all that apply)

Hands should be wet prior to adding soap so that soap will go into solution and produce more lather. Also, applying soap to dry skin may irritate the skin.

Which of the following are potential hazards which you might encounter in the laboratory? (Select all that apply) infectious microorganisms open flames hot surfaces corrosive chemicals sharp tools or broken glass gamma radiation magnetic fields

Infectious microorganisms, open flames, hot surfaces, corrosive chemicals and short tools or broken glass are all potential hazards when working in a lab.

Introductory microbiology laboratories are usually designated as which biosafety level? BSL-4 BSL 2 or BSL-3 BSL-1 or BSL-2 BSL-3 BSL-2

Introductory microbiology laboratories are usually designated as BSL-1 or BSL-2 facilities.

Jackie went to the sink at the end of lab to wash her hands. After removing her watch and rings, she turned on the water, wet her hands, added soap and thoroughly rubbed and lathered her hands for 30 seconds, cleaning the backs of her hands, her fingernails, and between her fingers. She then turned off the water, and reached for the paper towels to dry her hands. Identify the correct statement below.

Jackie should have used paper towels to turn off the faucet. After washing hands, paper towels should be used to turn off the faucet, since handling the faucet with clean hands will recontaminate the hands.

Which of the following represent important safety procedures for microbiology students? Always wear open-toed shoes so organisms do not become trapped in shoes. Wash your hands before and after laboratory procedures. Disinfect your lab bench at the beginning and end of the lab period. Only drink a closed container of water while conducting lab procedures. Take extra care around the flame of a Bunsen burner by tying up hair and keeping it from combustibles.

Never put anything in your mouth and always wear closed-toed shoes when in the lab!

Which of the following items may be on the laboratory bench top while doing lab work? (Select all that apply)

Only items directly related to the performance of the lab activity should be on the bench. Purses, backpacks, cell phones and any other personal items other than a lab manual and pencil or pen should be placed in a safe place, away from the lab bench. There should be no clutter on the bench.

Why is important to prepare a clean area before starting an experiment in the microbiology laboratory? To prevent infection and disease from environmental microbes To prevent contamination of cultures and samples To maintain sterility of the surface.

Prior to start an experiment in the microbiology laboratory, it is important to prepare a clean area to prevent contamination of cultures and samples.

Place the following steps of the hand washing procedure in the correct order.

Wet hands first, apply soap, lather for at least 20 seconds, rinse hands, dry hands, and then use the towel to turn the water off.

Which of the following steps are part of a thorough hand washing protocol? (Select all that apply)

When washing hands any jewelry or watches should be removed first. Soap should be applied to wet hands and turned into a lather while rubbing hands together for at least 20 seconds. If you have a brush or wooden stick near, cleaning under the finger nails should also occur.