Process of DNA Replication

Because hydrogen bonds are weak bonds, they create a perfect site for _____. a. separating nucleic acid strands b. attaching a helicase enzyme c. attaching a phosphodiester bond d. separating amino acid strands

a

What happens to the RNA primer once DNA replication is begun by DNA polymerase? a. The primer is removed. b. The primer becomes an enzyme. c. The primer becomes part of the daughter strand. d. The primer's length is doubled. e. The primer's length is tripled.

a

What is the purpose of the RNA primer? a. To initiate the process of DNA replication. b. To terminate the process of DNA replication. c. To build the daughter strand of DNA. d. To unwind the DNA double helix. e. To build the enzyme RNA primase.

a

Which word part is used to designate that a molecule is an enzyme? a. The suffix '-ase' b. The suffix '-amine' c. The prefix 'DNA' d. The suffix '-nucleic' e. The root 'polymer'

a

Why do we call DNA an antiparallel molecule? a. Because the 3' and 5' ends of each strand run in opposite directions. b. Because the 3' and 5' ends of each strand run in the same direction. c. Because DNA polymerase only works in the 3' to 5' direction. d. Because the 3' and 5' ends of each strand do not bond with each other. e. Because DNA is a perpendicular molecule.

a

The location where the process of DNA replication begins is called the _____. a. commencement of replication b. origin of replication c. genesis of replication d. initiation of replication

b

The replication of DNA is a complex process; all of the following statements are correct, EXCEPT a. On the lagging strand, one RNA primer is required for the beginning of every Okazaki fragment. b. There is one replication fork in one replication bubble. c. In order to complete replication, the replication bubbles grow and merge together. d. DNA replication is considered to be a semiconservative process.

b

What is the cellular process in which a new copy of DNA is created? a. Cell Division b. DNA Replication c. RNA Synthesis d. Dehydration Synthesis e. Protein Synthesis

b

What kind of organism was used to investigate the method of DNA Replication? a. Chimpanzee b. Bacteria c. Plant d. Fungus e. Mouse

b

Which enzyme is responsible for binding Okazaki fragments together? a. DNA amylase b. DNA ligase c. DNA helicase d. RNA primase e. DNA polymerase

b

Which is the strand that replicates just as quickly as the replication fork opens up? a. The daughter strand b. The leading strand c. The parent strand d. The lagging strand

b

Why is it necessary that the DNA helicase unwinds the DNA? a. It prevents DNA from breaking apart. b. It exposes the nitrogenous bases of the nucleic acid chain so that new nucleotides can be added to create the daughter strand. c. It exposes the phosphate groups of the nucleic acid chain so that new phosphates can be added to create the daughter strand. d. It prevents the replication of DNA.

b

The Dispersive method of DNA Replication was disproved when the scientists observed... a. one band of DNA of lowest possible density. b. one band of DNA of intermediate density. c. two separate bands of DNA of different densities. d. three separate bands of DNA of different densities. e. one band of DNA of highest possible density.

c

When DNA helicase separates the two parental strands, it creates a Y-shape called a _____. a. replication zipper b. replication helix c. replication fork d. replication split

c

Which of the following BEST explains the role of DNA polymerase? a. To build the RNA primer. b. To unwind the DNA double helix. c. To assemble daughter nucleotides on the parent strand. d. To join the adjacent Okazaki fragments.

c

Which of the following enzymes is in charge of unwinding the DNA double helix? a. DNA polymerase b. DNA topoisomerase c. DNA helicase d. DNA ligase

c

Why can't DNA polymerase work continuously on both parent strands? a. DNA polymerase only works in conjunction with DNA ligase. b. DNA polymerase builds a strand of DNA in the 3' to 5' direction, so it only works on the strand of DNA that runs in the 5' to 3' direction. c. DNA polymerase builds a strand of DNA in the 5' to 3' direction, so it only works on the strand of DNA that runs in the 3' to 5' direction. d. DNA polymerase only works in conjunction with RNA primase. e. DNA polymerase only works in conjunction with DNA helicase.

c

How many rounds of DNA Replication were needed to disprove the Conservative method? a. 5 b. 4 c. 2 d. 1

d

In the Meselson-Stahl experiment, why does N-15 DNA sink to a lower level than N-14 in a centrifuge tube? a. N-15 DNA has a lower density than N-14 DNA. b. N-15 has a higher melting point than N-14 DNA. c. N-15 DNA is more electronegative than N-14 DNA. d. N-15 DNA has a higher density than N-14 DNA. e. N-15 DNA has a higher volume than N-14 DNA.

d

In which method of DNA Replication would all parent DNA be conserved in the original molecule? a. Semi-Conductive b. Semi-Conservative c. Extracellular d. Conservative e. Dispersive

d

Using the given information, determine the correct order of the following events during the replication of the lagging strand. a. The DNA double helix unwinds. b. The Okazaki fragments are joined. c. The RNA primase builds an RNA primer on the parent strand. d. Nucleotides are added and matched to the parent strand. a. a, c, b, d b. c, a, d, b c. a, b, c, d d. a, c, d, b

d

Where are hydrogen bonds located within the DNA? a. On the backbone of the molecule linking the nitrogen bases to the deoxyribose b. In the center of the molecule linking the phosphorus and the deoxyribose c. On the backbone of the molecule linking the phosphate group and the deoxyribose sugar d. In the center of the molecule linking the nitrogen bases of both strands

d

Which enzyme is responsible for laying down the daughter nucleotides during DNA replication? a. RNA primase b. DNA helicase c. DNA ligase d. DNA polymerase

d

Which is the enzyme that attaches daughter nucleotides to the parent strand during DNA replication? a. DNA Primase b. DNA Helicase c. RNA Primase d. DNA Polymerase e. RNA Polymerase

d

Which of the parent DNA strands is replicated discontinuously? a. the daughter strand b. the leading strand c. the polymerase strand d. the lagging strand e. the helicase strand

d

Who performed the experiment that proved semi-conservative DNA replication was correct? a. James Watson and Francis Crick b. Erwin Chargaff c. Charles Darwin d. Matthew Meselson and Franklin Stahl e. Rosalind Franklin

d

How is the N-15 isotope different from the N-14 atom? a. N-15 has one extra proton. b. N-15 has one less neutron than N-14. c. N-15 has one less proton than N-14. d. N-15 has one extra electron. e. N-15 has one extra neutron.

e

In which method of DNA Replication would new DNA consist of alternating parent and daughter DNA? a. Conservative b. Extracellular c. Semi-Conservative d. Semi-Conductive e. Dispersive

e

What are the small components (monomers) that make up the large DNA polymer? a. Nucleosomes b. Nucleic Acids c. Guanine d. Cytosine e. Nucleotides

e

Which is the enzyme that builds the RNA primer? a. DNA Primase b. RNA Polymerase c. DNA Polymerase d. DNA Helicase e. RNA Primase

e