Uworld: Molecular Biology

What are the two types of Transcription factors?

1. Activators: Increase Transcription 2. Repressors: Decrease Transcription

What is the function of DNA Polymerase in cRNA cloning?

1. Amplifies the cDNA

What kind of DNA and replication fork does a prokaryotic have?

1. Circular DNA with a single origin of replication of the cytoplasm with two replication forks

What is the function of DNA Synthesis?

1. Create DNA molecules by assembling nucleotides

What of DNA and replication fork does a eukaryotic have?

1. Eukaryotes have linear DNA with multiple DNA origins

What is the role of Restriction Enzyme in cRNA cloning?

1. Foreign cDNA and vector are cut by a restriction enzyme, making complementary sticky ends that have both molecules together

What is made is the function of DNA Ligase in cDNA cloning?

1. Joins cDNA to the vector, making a vector that can be inserted into cells

What is the role of topoisomerase in DNA replication?

1. Preventing DNA from getting tangled!

What is the function of Primase? "Primary"- beginning

1. Primase gives a starting point for each DNA Strand

Which of the following are part of cDNA?

1. Reverse Transcriptase 2. RNA Polymerase 3. DNA Polymerase

What is DNA Ligase? Ligation Nation

1. Unites Ozaki fragments

What is the function of DNA Helicase?

1. Unwinds the DNA double helix to separate the 2 strands

What is the function of Reverse Transcriptase in cRNA cloning?

1. Use dNTP's (Nucleotide structure) to generate single stranded cDNA molecules from target mRNAS

What is cDNA cloning?

1. cDNA: a. Circular DNA molecules found in bacteria and archaea

What does the Transcription Factor: Activator do?

Activators facilitate: 1. RNA polymerase binding to the promoter, and repressors inhibit binding.

After the 4-hour Aph treatment, recovery of stalled replication forks in BLM+ and BLM− cells was analyzed as a function of time. Which of the following conclusions best explains the data shown in the graph below? There is a graph that is given: A. The time at which replication fork recovery was assessed serves to predict BLM expression levels in both cell types. B.The rates of DNA synthesis at recovering replication forks differs in the two cell types. C.BLM− cells are less sensitive to the effects of Aph than BLM+ cells. D.BLM activity in the two groups is primarily dependent on the percentage of replication forks that can be rescued.

B. The rates of DNA synthesis at recovering replication forks differ in the two cell types. (55%) 1. This question asks what happens between the MlB- and the MLB+ cells. 2. BLM+ is present a. Higher Fork Recovery b. Higher DNA synthesis The bar graph increases with BLM+ 3. BLM- is absent a. Lower Fork Recovery b. Lower DNA synthesis The bar graph decreases with BLM-

What is the function of Single Stranded DNA?

Binds to each strand to prevent spontaneous reformation of unwound single stranded DNA

Somatic Cells most commonly cause what kind of disease?

Cancer