Bio 151L Questions

Why did the LB/Amp (-) plasmid plate serve as a negative control?

A) To ensure that the untransformed E. coli are ampicillin sensitive. B) To ensure that the ampicillin is effective.

All of the following questions can be answered scientifically, EXCEPT:

Can the environment in which a person is raised cause them to behave immorally?

Which cellular features differentiate plants from animals?

Chloroplast Cell wall Large central vacuole All of the above

True or False? Transcription and translation take place primarily within the nucleus.

False - translation occurs at the ribosomes in the cytoplasm or the ER.

A volcanic eruption that kills a large portion of a population is an example of

bottleneck effect

Which of the following is true in regard to the control group for an experiment:

It is when the independent variable is held at 0 or an established level

Who is credited with discovering the structure of DNA?

James Watson and Francis Crick

What three independent variables will we be manipulating in order to explore their effects on photosynthesis?

carbon dioxide, light intensity, light wavelength

Pleodorina is an aquatic green alga that is common in ponds, lakes, and roadside ditches. This organism is made up of 32 to 128 cells that are embedded in a gel-like matrix. In mature colonies, two types of cells can be distinguished: small somatic cells and larger reproductive cells that divide to form new colonies. Somatic cells carry on photosynthesis, but may become reproductive if isolated from the colony. Please decide how Pleodorina should be classified

colonial

Hormones, whether in plants or animals, always travel to their targets via circulating fluids.

false

True or False: There is no test for lactose tolerance.

false

True or False? In between each drug, the Daphnia will be allowed a 2 minute recovery period in distilled water.

false- they should be placed in ringer's solution

Referring to the handouts provided, which of the following birds are NOT discussed in the video.

galapagos penguin

We will be observing the effects of which plant hormones on Solanum lycopersicum?

gibberellic acid

To begin Exercise 1, Experiment B, which of the following will be placed INSIDE the dialysis tubing?

glucose and starch solution

What is the name of protein that the pGLO plasmid codes for?

green fluorescent protein

The pGLO plasmid has been genetically engineered to include genes for all of the following traits EXCEPT:

heat resistance

When we complete Diffusion and Osmosis Experiment 3, Exericse A (the potato osmolarity experiment), where on the Y-axis would we place zero for our results graph?

in the middle of the y-axis

What advantage does the process of crossing over bring to reproduction?

introduces genetic variation

In the light microscope, the _______________ controls the width of light passing through the specimen.

iris diaphragm

What was the purpose of the 'lanolin' treatment in the experiment using the tomato plants?

Lanolin was a vehicle that was used to deliver auxin and gibberelin to the experimental plants, and had to be used on the control plants.

What is the name of protein that the pGLO plasmid codes for?

lb/amp -plasmid

__________________ is best described as one round of replication and two rounds of cell division.

meiosis

During which phase of the cell cycle do the sister chromatids align at the equator of the cell?

metaphase

While cooking ramen noodles, you burn your finger on the stove. What process is involved to regenerate the skin cells on your fingertip?

mitosis

What did we expect to see on the plate with LB and ampicillin when we plated the non-transformed bacteria on it?

no growth

For next week's lab, what is being added to the -plasmid tube?

nothing

What product of photosynthesis will we be using to measure photosynthesis rates in the spinach leaf disks?

oxygen

Which of the following was not an independent variable we looked at when measuring photosynthesis?

oxygen

Evolution occurs in:

populations

___________ cells lack membrane-bound organelles.

prokaryotic

DNA contains all of the following EXCEPT:

protein

Penicillin is an antibiotic that inhibits the synthesis of peptidoglycan, an important part of the cell wall of bacteria. This causes the cell wall to break down, and the bacteria to burst. Using your knowledge from laboratory, which of the following do you think is TRUE:

Penicillin is an enzyme inhibitor

Which of the following best represents how cells progress through the M phase of the cell cycle?

Prophase; Prometaphase; Metaphase; Anaphase; Telophase; Cytokinesis

Assume that while conducting the Daphnia experiment you mistakenly overdose the organism with a given drug. As a result the Daphnia dies. What is the best practice when you start collecting data with a new Daphnia?

Repeat the baseline heartrate measurement with the new Daphnia

Which of the following is true regarding sampling with replacement?

Sampling with replacement is when the selected sample is placed back into the population before the next sample is chosen

Which of the following is NOT a drug we will be using with the Daphnia in this coming week's lab?

THC

In Exercise 3, Experiment A, (starting on page 92) we observed the influence of enzyme concentration on the rate of starch digestion. What was the dependent variable in this experiment? When constructing a graph, what is the appropriate axis for the dependent variable?

reaction, y-axis

Referring to the handouts provided, what does sickle cell anemia affect?

red blood cell shape

What three wavelengths of light will we be filtering to test for the effects on photosynthesis rates?

red, green, blue

During which phase of the cell cycle are chromosomes replicated?

s phase

Which order illustrates the evolutionary trend of organisms from less to more complex?

single celled -> aggregate -> simple colonial -> complex colonial -> multicellular

In the upcoming laboratory session, you will test Hardy-Weinberg equilibrium by simulating a population using colored beads. The bag of beads will represent the gene pool for the population. Each bead should be regarded as a ________, the two colors representing different _________.

single, gamete, allele

In order for allelic frequencies to remain unchanged within a population, all of the following conditions must be met EXCEPT:

small population

During lab this week, we used a chemical substance called catechol. In our experiment, this substance can best be categorized as a/an:

substrate

The products of photosynthesis are:

sugar and oxygen

The sheep "Dolly" was the first mammal cloned by transplanting a nucleus from a cell in an adult sheep into an egg of a donor sheep. Dolly appeared to grow normally for several years, but at age 6, she developed complications from several physical conditions normally only seen in older sheep. Although, there are many hypotheses about the reason Dolly suffered from traditionally old-age, one hypothesis seems to fit better than the others. Dolly's physical condition was likely due to issues associated with:

telomere length

With respect to microscope lenses, parfocal means _______.

that little focusing is required when moving from one lens to another.

What chemical was left out of solution for the 'No CO2' treatment?

NaHCO3

Why would it be wise to observe the Daphnia under lower light intensity?

Overheating the Daphnia could stress and injure them

All of the following are TRUE in regard to Exercise 2 (starting on page 89) EXCEPT:

PTU was added to the test tubes last to ensure a proper reaction

Using the equation p+q=1, if the frequency of allele p=0.8, the frequency of the allele q=

0.2

Referring to the handouts provided, how many species of finches exist on the Galapagos Islands?

13

Which of the following is NOT required for translation to occur?

DNA

Which of the following is NOT true regarding DNA?

DNA contains equal numbers of adenine and uracil bases

Which of the following is NOT a difference between DNA and RNA?

DNA is negatively charged and RNA is positively charged.

All of the following are TRUE regarding the video about Pocket Mice, EXCEPT:

Genetic mutations are always harmful to an organism

In geographic areas with a high incidence of malaria, which of the following sickle cell genotypes is selected for?

Heterozygous (sickle cell trait)

Given the results of your experiment, under what conditions would spinach in your garden grow the best?

High levels of CO2, high intensity of light

Which of the following is TRUE regarding meiosis:

If the parent cell is 2n=16, when the homologous pairs form a synapsis during prophase I, there will be 8 tetrad complexes

All of the following are true regarding mitosis, EXCEPT:

In plant cells, the cleavage furrow divides the cytoplasm during cytokinesis

What would the amino acid sequence of the following mRNA be translated to? Hint: use the mRNA codon table in your handout. mRNA: 5'-GUUAAUGGCUGGUUUUUAA-3'

Met-Ala-Gly- Phe-Stop

The cells of an Elodea leaf become turgid when placed in a hypotonic solution. Why?

The cells have a higher solute concentration than their surroundings. Water is drawn into the cell and the protoplast presses on the cell wall.

Regarding Exercise 3, Experiment C (starting on page 100), all of the following are true EXCEPT

The enzyme that was placed on ice was denatured

Which of the following is TRUE in regard to tables:

The units of measurement should be included in the heading

In the E. coli transformation exercise, what did we hope to accomplish?

The uptake and propagation of the pGLO plasmid in the E. coli cytosol in order to confer ampicillin resistance

All of the following are TRUE in regard to Stereoscopic microscopes EXCEPT

They cannot be used to view objects too thick to transmit light

Using Figure 5 on page 20 of your lab manual, ALL of the following are true EXCEPT:

This graph proves the hypothesis that men and women aged 20-39 consume a greater than average amount of their calories from fast food.

What is the purpose of sucking the spinach leaf disks using a large plastic syringe?

To remove all O2 from the leaves.

Rosalind Franklin's photo 51 was critical X-ray crystallography data for the discovery of the double helix. What did photo 51 show?

an "x"

One strand of DNA runs in a 5I to 3I direction, while the other strand runs 3I to 5I. The term used to describe this phenomenon is _____?

antiparallel

Which of the following characteristics did Darwin note on finches' beaks, if their primary food source was seeds?

beak was thicker, stronger

Which test indicates the presence of a reducing sugar by changing from blue to green/orange/orange-red when heated.

benedict's test

Assuming the experiment worked as expected, which of the following wavelengths of light showed the greatest oxygen production?

blue

How will we be measuring oxygen production in the spinach leaf disks?

counting the number of discs that are floating

What is the appropriate term given to red blood cells placed into a hypertonic solution?

crenate

The cell in Figure 9a on page 115 is in which phase?

cytokinesis

Which of the following plant hormones causes leaf abscission?

d ethylene

What is the model organism we are using for next week's lab?

e. coli

What was the significance of returning the Daphnia to Ringers solution between treatments?

to allow the daphnia to recover from the treatment

What is the term for the procedure where foreign DNA is inserted into a bacterial cell.

transformation

TRUE/FALSE: Sickle cell disease is more prevalent in the African American population than in Caucasians.

true

True or False? All the organisms we observed using the microscopes in lab this past week were eukaryotic.

true

True or False? In both DNA replication and transcription of mRNA, the newly formed nucleotide strand is always synthesized in the 5I to 3I direction.

true

True or False? When placing the apples and holly twigs underneath the 1L beakers, it is important that the beaker lips are covered with sand so that no gas can enter or escape the beakers.

true

True or false: If the results of the experiment match the prediction, then the hypothesis is supported.

true

True or false: If the somatic cells (referred to in the previous question) are isolated from the colony, they can become reproductive.

true

True or false: In colonial algae, somatic cells are cells that are not reproductive

true

Which of the following steps in the procedure was specifically performed to promote the uptake of the plasmid by the E. coli?

using a solution called calcium chloride

Referring to the handouts provided, the Pocket Mice studied in the video that we will watch are found where?

valley of fire