Bio Lab quiz

DNA isolation process- how is DNA stabilized

The test tube was placed in an ice bath

Isolate DNA from strawberries

1) Transfer strawberry filtrate to text tube 2) Dissolve meat tenderize and ass it to test tube. 3) /add ice cole ethanol to test tube. 4) Wait for DNA precipitate to form 5) Spool DNA precipitate onto glass rod

Why does DNA flow toward the positive side of the gel chamber

DNA has a negative charge and is attracted by the pos. side.

Meat tenderizer

DNA isolation process, what is used to break down the protein complexes and allow the DNA molecules to easily precipitate.

Process of breaking down large fat globules into smaller fat globules.

Emulsification

Detergent for DNA isolation

Emulsify cellular and nuclear membranes

DNA Isolation

Extraction of DNA from viruses or cells

Homogenization

First step in DNA isolation. It refers to the process of releasing DNA from the cells.

gel electrophoresis/ True

Important tool in biotechnology for forensics and paternity test.

In order to prevent the DNA from breaking, the glass rob should be spun

In one direction

Why is bromophenol blue added to the individual DNA samples.

It allows the observer to view how far the DNA samples travel.

Ethanol is used in the DNA isolation process

It allows the precipitate to form

In gel electrophoresis, how are the DNA samples added to the walls?

Micropipet is used.

Buffer is poured over the gel because it improves conduction of the electric current

Purpose of electrophoresis

Collecting the DNA. Can be collected on a glass rod in a technique

Spooling

DNA isolation process, cells are mixed with sodium chloride. NaCl neutralizes the negative charge of DNA.

True

Ethanol cannot be added to the test tube too quickly because it will break up the DNA precipitate.

True

Biotechnology

Use of living organisms to perform a task that benefits humans and other living organisms.

Nucleus

Where DNA is located within the eukaryotic cell