Ch 3: The Five I's
General Purpose Media
A Functional Type of media that allows for the growth of as broad a spectrum of microbes as possible and is usually made of NONSYNTHETIC media containing a mixture of nutrients to support a variety of microbes:
Enriched Media
A Functional Type of media that contains complex organic substances (ie. blood, serum, growth factors) to support the growth of FASTIDIOUS bacteria (FASTIDIOUS=needs specialized environment to support nutritional requirements).
Carbohydrate Fermentation Media
A Functional Type of media that contains sugars that can be fermented (converted to acids) and a pH indicator to show this reaction.
Transport media
A Functional Type of media that is used to maintain and preserve specimens that need to be held for a period of time.
Differential Media
A Functional type of media that allows multiple types of microorganisms to grow but display visible differences among those microorganisms.
Reducing Medium
A Functional type of media that contains a substance that adsorbs oxygen or slows the penetration of oxygen in a medium, thus diminishing its availability. These media are important for growing anaerobic bacteria or for determining oxygen requirements of isolates.
Selective Media
A Functional type of media that contains one or more agents that inhibit the growth of certain microbes but not others.
Nonsynthetic
Blood, serum, and meat extracts or infusions are examples of what kind of CHEMICAL media?
Loop Dilution Method
Method of inoculating where a sample is inoculated serially into a series of cooled but still liquid agar tubes so as to dilute the number of cells in each successive tube in the series.
Streak Plate method
Method of inoculating where a small droplet of culture or sample is spread over a surface of a medium with an inoculating loop. In this technique, a pattern is used that thins out the sample and separates the cells.
Spread Plate Method
Method of inoculating where a small volume of liquid-diluted sample is pipetted onto the surface of a medium and spread around evenly by a sterile spreading too.
Identification
One of the FIVE I's that involves determining the type of microbe to the level of species.
Inoculation
One of the FIVE I's that involves introducing a tiny sample into a container of nutrient medium.
Inspection
One of the FIVE I's that involves observing growth characteristics that could be useful in analyzing the specimen contents such as appearance and metabolism. (Staining techniques are used in this step).
Incubation
One of the FIVE I's that involves placing an inoculated medium in an environment that supports proper growth temperature and other conditions.
Isolation
One of the FIVE I's that involves separating one species from another allowing the formation of a pure culture that contains only a single species of microbe.
Synthetic, Nonsynthetic
What are the 2 types of Chemical compositions of media?
Liquid, Semisolid, Solid (can be converted to liquid), Solid (cannot be converted to liquid)
What are the 4 types of Physical State media?
Inoculation, incubation, Isolation, Inspection, Identification
What are the Five I's (in order), that describe how a sample is processed and profiled?
Physical State, Chemical Composition, Functional Type
What are the three categories of media classification?
Nonsynthetic
What type of chemical media is considered "Complex" and contains components that are not chemically definable?
Synthetic
What type of chemical media is precisely chemically defined?
Liquid
What type of physical state media are water-based solutions that do NOT solidify at temperatures above freezing, and flow freely when container is tilted?
Semisolid
What type of physical state media have a clot-like consistency at room temperature and are used to determine MOTILITY and to localize reactions at a specific site?