Chapter 16
Indicate by writing "yes" or "no" whether amplification of a signal could occur at the particular steps described below. C. Adenylyl cyclase produces cyclic AMP.
Yes
Indicate by writing "yes" or "no" whether amplification of a signal could occur at the particular steps described below. E. Protein kinase A phosphorylates target proteins
Yes
Indicate by writing "yes" or "no" whether amplification of a signal could occur at the particular steps described below. B. The activated GPCRs cause Gα to separate from Gβ and Gγ.
Yes
Acetylcholine binds to a GPCR on heart muscle, making the heart beat more slowly. The activated receptor stimulates a G protein, which opens a K+ channel in the plasma membrane, as shown in Figure Q16-32. Which of the following would enhance this effect of the acetylcholine? Figure Q16-32 (a) addition of a high concentration of a nonhydrolyzable analog of GTP (b) addition of a drug that prevents the α subunit from exchanging GDP for GTP (c) mutations in the acetylcholine receptor that weaken the interaction between the receptor and acetylcholine (d) mutations in the acetylcholine receptor that weaken the interaction between the receptor and the G protein
a
Activated protein kinase C (PKC) can lead to the modification of the membrane lipids in the vicinity of the active PKC. Figure Q16-38 shows how G proteins can indirectly activate PKC. You have discovered the enzyme activated by PKC that mediates the lipid modification. You call the enzyme Rafty and demonstrate that activated PKC directly phosphorylates Rafty, activating it to modify the plasma membrane lipids in the vicinity of the cell where PKC is active; these lipid modifications can be detected by dyes that bind to the modified lipids. Cells lacking Rafty do not have these modifications, even when PKC is active. Which of the following conditions would lead to signal-independent modification of the membrane lipids by Rafty? Figure Q16-38 (a) the expression of a constitutively active phospholipase C (b) a mutation in the GPCR that binds the signal more tightly (c) a Ca2+ channel in the endoplasmic reticulum with an increased affinity for IP3 (d) a mutation in the gene that encodes Rafty such that the enzyme can no longer be phosphorylated by PKC
a
The ethylene response in plants involves a dimeric transmembrane receptor. When the receptor is not bound to ethylene, the receptor binds to and activates a protein kinase, which activates an intracellular signaling pathway that leads to the degradation of a transcriptional regulator important for transcribing the ethyleneresponsive genes (see Figure Q16-60). You discover a phosphatase that is important for ethylene signaling, and you name it PtpE. Plants lacking PtpE never turn on ethylene-responsive genes, even in the presence of ethylene. You find that PtpE dephosphorylates serine 121 on the transcriptional regulator. Furthermore, plants lacking PtpE degrade the transcriptional regulator in the presence of ethylene. Figure Q16-60 Which of the following statements is inconsistent with your data? (a) When the transcriptional regulator is phosphorylated, it activates transcription of the ethylene-responsive genes. (b) When the transcriptional regulator is not phosphorylated, it binds to DNA. (c) Activation of the protein kinase that binds to the ethylene receptor leads to inactivation of PtpE. (d) Binding of ethylene to its receptor leads to the activation of PtpE.
a
Match the class of cell-surface receptor with the best description of its function. Not all descriptors will be used. G-protein-coupled receptors ______ ion-channel-coupled receptors ______ enzyme-coupled receptors ______ A. alter the membrane potential directly by changing the permeability of the plasma membrane B. signal by opening and closing in a ligand-independent manner C. must be coupled with intracellular monomeric GTP-binding proteins D. all receptors of this class are polypeptides with seven transmembrane domains E. discovered for their role in responding to growth factors in animal cells
D, A, E
Indicate whether each of the following conditions would increase or decrease the effect of acetylcholine. A. addition of a drug that stimulates the GTPase activity of the Gα subunit
Decrease
Indicate whether each of the following conditions would increase or decrease the effect of acetylcholine. B. mutations in the K+ channel that keep it closed all the time
Decrease
Indicate whether each of the following conditions would increase or decrease the effect of acetylcholine. D. a mutation that decreases the affinity of the βγ complex of the G protein for the K+ channel
Decrease
Indicate whether each of the following conditions would increase or decrease the effect of acetylcholine. E. a mutation in the acetylcholine receptor that prevents its localization on the cell surface
Decrease
Indicate whether each of the following conditions would increase or decrease the effect of acetylcholine. F. adding acetylcholinesterase to the external environment of the cell
Decrease
Cells signal to one another in various ways. Some use extracellular signal molecules that are dissolved gases, such as __________________, which can diffuse easily into cells. Others use cytokines, which bind to cytokine receptors. Cytokine receptors have no intrinsic enzyme activity but are associated with cytoplasmic tyrosine kinases called __________________s, which become activated on the binding of cytokine to its receptor and go on to phosphorylate and activate cytoplasmic transcriptional regulators called __________________s. Some intracellular signaling pathways involve chains of protein kinases that phosphorylate each other, as seen in the __________________ signaling module. Lipids can also relay signals in the cell, as we observe when phospholipase C cleaves the sugar-phosphate head off a lipid molecule to generate the two small messenger molecules __________________ (which remains embedded in the plasma membrane) and __________________ (which diffuses into the cytosol). cyclic GMP MAP kinase STAT diacylglycerol NO TGFβ IP3 Ras JAK SMAD
NO, JAKs, STATs, MAP kinase, diacylglycerol, IP3
Indicate by writing "yes" or "no" whether amplification of a signal could occur at the particular steps described below. D. cAMP activates protein kinase A
No
When Ras is activated, cells will divide. A dominant-negative form of Ras clings too tightly to GDP. You introduce a dominant-negative form of Ras into cells that also have a normal version of Ras. Which of the following statements is true? (a) The cells you create will divide less frequently than normal cells in response to the extracellular signals that typically activate Ras. (b) The cells you create will run out of the GTP necessary to activate Ras. (c) The cells you create will divide more frequently compared to normal cells in response to the extracellular signals that typically activate Ras. (d) The normal Ras in the cells you create will not be able to bind GDP because the dominant-negative Ras binds to GDP too tightly
a
Which of the following mechanisms is not directly involved in inactivating an activated RTK? (a) dephosphorylation by serine/threonine phosphatases (b) dephosphorylation by protein tyrosine phosphatases (c) removal of the RTK from the plasma membrane by endocytosis (d) digestion of the RTK in lysosomes
a
Which of the following statements about molecular switches is false? (a) Phosphatases remove the phosphate from GTP on GTP-binding proteins, turning them off. (b) Protein kinases transfer the terminal phosphate from ATP onto a protein. (c) Serine/threonine kinases are the most common types of protein kinase. (d) A GTP-binding protein exchanges its bound GDP for GTP to become activated.
a
Which of the following statements is true? (a) Extracellular signal molecules that are hydrophilic must bind to a cellsurface receptor so as to signal a target cell to change its behavior. (b) To function, all extracellular signal molecules must be transported by their receptor across the plasma membrane into the cytosol. (c) A cell-surface receptor capable of binding only one type of signal molecule can mediate only one kind of cell response. (d) Any foreign substance that binds to a receptor for a normal signal molecule will always induce the same response that is produced by that signal molecule on the same cell type
a
You are interested in cell-size regulation and discover that signaling through a GPCR called ERC1 is important in controlling cell size in embryonic rat cells. The G protein downstream of ERC1 activates adenylyl cyclase, which ultimately leads to the activation of PKA. You discover that cells that lack ERC1 are 15% smaller than normal cells, while cells that express a mutant, constitutively activated version of PKA are 15% larger than normal cells. Given these results, which of the following treatments to embryonic rat cells should lead to smaller cells? (a) addition of a drug that causes cyclic AMP phosphodiesterase to be hyperactive (b) addition of a drug that prevents GTP hydrolysis by Gα (c) addition of a drug that activates adenylyl cyclase (d) addition of a drug that mimics the ligand of ERC1
a
Which of the following statements is true? (a) Because endocrine signals are broadcast throughout the body, all cells will respond to the hormonal signal. (b) The regulation of inflammatory responses at the site of an infection is an example of paracrine signaling. (c) Paracrine signaling involves the secretion of signals into the bloodstream for distribution throughout the organism. (d) The axons of neurons typically signal target cells using membrane-bound signaling molecules that act on receptors in the target cells.
b
Which of the following statements is true? (a) MAP kinase is important for phosphorylating MAP kinase kinase. (b) PI 3-kinase phosphorylates a lipid in the plasma membrane. (c) Ras becomes activated when an RTK phosphorylates its bound GDP to create GTP. (d) STAT proteins phosphorylate JAK proteins, which then enter the nucleus and activate gene transcription.
b
You are interested in further understanding the signal transduction pathway that controls the production of Pig1, a protein important for regulating cell size. Activation of the TRK receptor leads to activation of the GTP-binding protein, Ras, which then activates a protein kinase that phosphorylates the SZE transcription factor. SZE only interacts with the nuclear transport receptor when it is phosphorylated. SZE is a gene activator for the Pig1 gene. This pathway is diagrammed in Figure Q16-50. Figure Q16-50 Normal cells grown under standard conditions (without ligand) are 14 µm in diameter while normal cells exposed to TRK ligand are 10.5 µm in diameter. Given this situation, which of the following conditions do you predict will more likely lead to smaller cells? (a) addition of TRK ligand and a drug that stimulates the GTPase activity of Ras (b) addition of TRK ligand and a drug that inhibits the activity of the phosphatase that acts on SZE (c) addition of TRK ligand and a drug that stimulates the degradation of Pig1 (d) addition of TRK ligand and a drug that inhibits Pig1 binding to DNA
b
Name the three main classes of cell-surface receptor
ion-channel-coupled receptors; G-protein-coupled receptors; enzyme-coupled receptors
Figure Q16-61 shows that intracellular signaling pathways can be highly interconnected. Figure Q16-61 From the information in Figure Q16-61, which of the following statements is incorrect? (a) The GPCR and the RTK both activate phospholipase C. (b) Activation of either the GPCR or the RTK will lead to activation of transcriptional regulators. (c) CaM-kinase is only activated when the GPCR is active and not when the RTK is active. (d) Ras is activated only when the RTK is active and not when the GPCR is active.
c
Figure Q16-63 shows how normal signaling works with a Ras protein acting downstream of an RTK. You examine a cell line with a constitutively active Ras protein that is always signaling. Which of the following conditions will turn off signaling in this cell line? Figure Q16-63 (a) addition of a drug that prevents protein X from activating Ras (b) addition of a drug that increases the affinity of protein Y and Ras (c) addition of a drug that blocks protein Y from interacting with its target (d) addition of a drug that increases the activity of protein Y
c
Foreign substances like nicotine, morphine, and menthol exert their initial effects by _____. (a) killing cells immediately, exerting their physiological effects by causing cell death. (b) diffusing through cell plasma membranes and binding to transcription factors to change gene expression. (c) interacting with cell-surface receptors, causing the receptors to transduce signal inappropriately in the absence of the normal stimulus. (d) removing cell-surface receptors from the plasma membrane.
c
The following happens when a G-protein-coupled receptor activates a G protein. (a) The β subunit exchanges its bound GDP for GTP. (b) The GDP bound to the α subunit is phosphorylated to form bound GTP. (c) The α subunit exchanges its bound GDP for GTP. (d) It activates the α subunit and inactivates the βγ complex.
c
The growth factor Superchick stimulates the proliferation of cultured chicken cells. The receptor that binds Superchick is a receptor tyrosine kinase (RTK), and many chicken tumor cell lines have mutations in the gene that encodes this receptor. Which of the following types of mutation would be expected to promote uncontrolled cell proliferation? (a) a mutation that prevents dimerization of the receptor (b) a mutation that destroys the kinase activity of the receptor (c) a mutation that inactivates the protein tyrosine phosphatase that normally removes the phosphates from tyrosines on the activated receptor (d) a mutation that prevents the binding of the normal extracellular signal to the receptor
c
The lab you work in has discovered a previously unidentified extracellular signal molecule called QGF, a 75,000-dalton protein. You add purified QGF to different types of cells to determine its effect on these cells. When you add QGF to heart muscle cells, you observe an increase in cell contraction. When you add it to fibroblasts, they undergo cell division. When you add it to nerve cells, they die. When you add it to glial cells, you do not see any effect on cell division or survival. Given these observations, which of the following statements is most likely to be true? (a) Because it acts on so many diverse cell types, QGF probably diffuses across the plasma membrane into the cytoplasm of these cells. (b) Glial cells do not have a receptor for QGF. (c) QGF activates different intracellular signaling pathways in heart muscle cells, fibroblasts, and nerve cells to produce the different responses observed. (d) Heart muscle cells, fibroblasts, and nerve cells must all have the same receptor for QGF.
c
The local mediator nitric oxide stimulates the intracellular enzyme guanylyl cyclase by ________________. (a) activating a G protein. (b) activating a receptor tyrosine kinase. (c) diffusing into cells and stimulating the cyclase directly. (d) activating an intracellular protein kinase.
c
Ca2+ can trigger biological effects in cells because an unstimulated cell has an extremely __________________ concentration of free Ca2+ in the cytosol, compared with its concentration in the __________________ space and in the __________________, creating a steep electrochemical gradient. When Ca2+ enters the cytosol, it interacts with Ca2+-responsive proteins such as __________________, which also binds diacylglycerol, and __________________, which activates CaM-kinases. adenylyl cyclase endoplasmic reticulum nuclear average extracellular peroxisome Ca2+ high phospholipase C calmodulin intracellular protein kinase A colorful low protein kinase C
low, extracellular, endoplasmic reticulum, protein kinase C, calmodulin
You are interested in how cyclic-AMP-dependent protein kinase A (PKA) functions to affect learning and memory, and you decide to study its function in the brain. It is known that, in the cells you are studying, PKA works via a signal transduction pathway like the one depicted in Figure Q16-35. Furthermore, it is also known that activated PKA phosphorylates the transcriptional regulator called Nerd that then activates transcription of the gene Brainy. Which situation described below will lead to an increase in Brainy transcription? Figure Q16-35 (a) a mutation in the Nerd gene that produces a protein that cannot be phosphorylated by PKA (b) a mutation in the nuclear import sequence of PKA from PPKKKRKV to PPAAAAAV (c) a mutation in the gene that encodes cAMP phosphodiesterase that makes the enzyme inactive (d) a mutation in the gene that encodes adenylyl cyclase that renders the enzyme unable to interact with the α subunit of the G protein
c
An extracellular signal molecule can act to change a cell's behavior by acting through cell-surface __________________ that control intracellular signaling proteins. These intracellular signaling proteins ultimately change the activity of __________________ proteins that bring about cell responses. Intracellular signaling proteins can __________________ the signal received to evoke a strong response from just a few extracellular signal molecules. A cell that receives more than one extracellular signal at the same time can __________________ this information using intracellular signaling proteins. __________________ proteins can act as molecular switches, letting a cell know that a signal has been received. Enzymes that phosphorylate proteins, termed ___________, can also serve as molecular switches; the actions of these enzymes are countered by the activity of __________________. acetylase decouple GTP-binding AMP-binding decrease neurotransmitter amplify effector protein kinases autocrine esterases protein phosphatases cleavage integrate receptors convolute GMP-binding sterols
receptors, effector, amplify, integrate, GTP-binding, protein kinases, protein phosphatases
G-protein-coupled receptors (GPCRs) all have a similar structure with __________________ transmembrane domains. When a GPCR binds an extracellular signal, an intracellular G protein, composed of __________________ subunits, becomes activated. __________________ of the G-protein subunits are tethered to the plasma membrane by short lipid tails. When unstimulated, the α subunit is bound to __________________, which is exchanged for __________________ on stimulation. The intrinsic __________________ activity of the α subunit is important for inactivating the G protein. __________________ inhibits this activity of the α subunit, thereby keeping the subunit in an active state. adenylyl cyclase cholera toxin GTPase AMP diacylglycerol phosphodiesterase ATP five seven ATPase four three Ca2+ GDP twelve cAMP GTP two
seven, three, two, GDP, GTP, GTPase, Cholera toxin
Akt promotes the survival of many cells by affecting the activity of Bad and Bcl2, as diagrammed in Figure Q16-58. Figure Q16-58 Which of the following statements is false? (a) In the presence of a survival signal, Akt is phosphorylated. (b) In the absence of a survival signal, Bad inhibits the cell-death inhibitor protein Bcl2. (c) In the presence of a survival signal, the cell-death inhibitory protein Bcl2 is active. (d) In the absence of a survival signal, Bad is phosphorylated
d
All members of the steroid hormone receptor family __________________. (a) are cell-surface receptors. (b) do not undergo conformational changes. (c) are found only in the cytoplasm. (d) interact with signal molecules that diffuse through the plasma membrane.
d
During nervous-system development in Drosophila, the membrane-bound protein Delta acts as an inhibitory signal to prevent neighboring cells from developing into neuronal cells. Delta is involved in ______________ signaling. (a) endocrine (b) paracrine (c) neuronal (d) contact-dependent
d
The length of time a G protein will signal is determined by _______. (a) the activity of phosphatases that turn off G proteins by dephosphorylating Gα. (b) the activity of phosphatases that turn GTP into GDP. (c) the degradation of the G protein after Gα separates from Gβγ. (d) the GTPase activity of Gα.
d
Which of the following statements about G-protein-coupled receptors (GPCRs) is false? (a) GPCRs are the largest family of cell-surface receptors in humans. (b) GPCRs are used in endocrine, paracrine, and neuronal signaling. (c) GPCRs are found in yeast, mice, and humans. (d) The different classes of GPCR ligands (proteins, amino acid derivatives, or fatty acids) bind to receptors with different numbers of transmembrane domains.
d
Which of the following statements is false? (a) Nucleotides and amino acids can act as extracellular signal molecules. (b) Some signal molecules can bind directly to intracellular proteins that bind DNA and regulate gene transcription. (c) Some signal molecules are transmembrane proteins. (d) Dissolved gases such as nitric oxide (NO) can act as signal molecules, but because they cannot interact with proteins they must act by affecting membrane lipids
d
Cells can signal to each other in various ways. A signal that must be relayed to the entire body is most efficiently sent by __________________ cells, which produce hormones that are carried throughout the body through the bloodstream. On the other hand, __________________ methods of cell signaling do not require the release of a secreted molecule and are used for very localized signaling events. During __________________ signaling, the signal remains in the neighborhood of the secreting cell and thus acts as a local mediator on nearby cells. Finally, __________________ signaling involves the conversion of electrical impulses into a chemical signal. Cells receive signals through a __________________, which can be an integral membrane protein or can reside inside the cell. amplification G protein phosphorylation contact-dependent K+ channel receptor endocrine neuronal target epithelial paracrine
endocrine, contact-dependent, paracrine, neuronal, receptor
Given the generic signaling pathway in Figure Q16-12, write the number corresponding to the item on the line next to the descriptor below. Figure Q16-12 _________ receptor protein _________ effector proteins _________ intracellular signaling proteins _________ ligand
2, 4, 3, 1
Rank the following types of cell signaling from 1 to 4, with 1 representing the type of signaling in which the signal molecule travels the least distance and 4 the type of signaling in which the signal molecule travels the largest distance. ______ paracrine signaling ______ contact-dependent signaling ______ neuronal signaling ______ endocrine signaling
3, 1, 2, 4
Indicate by writing "yes" or "no" whether amplification of a signal could occur at the particular steps described below. Explain your answers. A. An extracellular signaling molecule binds and activates a GPCR.
A. No
Circle the phrase in each pair that is likely to occur more rapidly in response to an extracellular signal. A. changes in cell secretion / increased cell division B. changes in protein phosphorylation / changes in proteins being synthesized C. changes in mRNA levels / changes in membrane potential
A. cell secretion B. protein phosphorylation C. membrane potential
When adrenaline binds to adrenergic receptors on the surface of a muscle cell, it activates a G protein, initiating an intracellular signaling pathway in which the activated α subunit activates adenylyl cyclase, thereby increasing cAMP levels in the cell. The cAMP molecules then activate a cAMP-dependent kinase (PKA) that, in turn, activates enzymes that result in the breakdown of muscle glycogen, thus lowering glycogen levels. You obtain muscle cells that are defective in various components of the signaling pathway. Referring to Figure Q16-36, indicate how glycogen levels would be affected in the presence of adrenaline in the following cells. Would they be higher or lower than in normal cells treated with adrenaline? A. cells that lack adenylyl cyclase B. cells that lack the GPCR C. cells that lack cAMP phosphodiesterase D. cells that have an α subunit that cannot hydrolyze GTP but can interact properly with the β and γ subunits
A. higher B. higher C. lower D. lower
Match the target of the G protein with the appropriate signaling outcome. adenylyl cyclase __________ ion channels __________ phospholipase C ___________ A. cleavage of inositol phospholipids B. increase in cAMP levels C. changes in membrane potential
B, C, A
You are interested in cell-size regulation and discover that signaling through an enzyme-coupled receptor is important for the growth (enlargement) of mouse liver cells. Activation of the receptor activates adenylyl cyclase, which ultimately leads to the activation of PKA, which then phosphorylates a transcription factor called TFS on threonine 42. This phosphorylation is necessary for the binding of TFS to its specific sites on DNA, where it then activates the transcription of Sze2, a gene that encodes a protein important for liver cell growth. You find that liver cells lacking the receptor are 15% smaller than normal cells, whereas cells that express a constitutively activated version of PKA are 15% larger than normal liver cells. Given these results, predict whether you would expect the cell's size to be bigger or smaller than normal cells if cells were treated in the following fashion B. You create a version of the receptor that is constitutively active.
Bigger
You are interested in cell-size regulation and discover that signaling through an enzyme-coupled receptor is important for the growth (enlargement) of mouse liver cells. Activation of the receptor activates adenylyl cyclase, which ultimately leads to the activation of PKA, which then phosphorylates a transcription factor called TFS on threonine 42. This phosphorylation is necessary for the binding of TFS to its specific sites on DNA, where it then activates the transcription of Sze2, a gene that encodes a protein important for liver cell growth. You find that liver cells lacking the receptor are 15% smaller than normal cells, whereas cells that express a constitutively activated version of PKA are 15% larger than normal liver cells. Given these results, predict whether you would expect the cell's size to be bigger or smaller than normal cells if cells were treated in the following fashion. E. You mutate the cAMP-binding sites in the regulatory subunits of PKA, so that the complex binds cAMP more tightly
Bigger
Indicate whether each of the following conditions would increase or decrease the effect of acetylcholine. C. modification of the Gα subunit by cholera toxin
Increase
You are interested in cell-size regulation and discover that signaling through an enzyme-coupled receptor is important for the growth (enlargement) of mouse liver cells. Activation of the receptor activates adenylyl cyclase, which ultimately leads to the activation of PKA, which then phosphorylates a transcription factor called TFS on threonine 42. This phosphorylation is necessary for the binding of TFS to its specific sites on DNA, where it then activates the transcription of Sze2, a gene that encodes a protein important for liver cell growth. You find that liver cells lacking the receptor are 15% smaller than normal cells, whereas cells that express a constitutively activated version of PKA are 15% larger than normal liver cells. Given these results, predict whether you would expect the cell's size to be bigger or smaller than normal cells if cells were treated in the following fashion C. You add a drug that inhibits adenylyl cyclase
Smaller
You are interested in cell-size regulation and discover that signaling through an enzyme-coupled receptor is important for the growth (enlargement) of mouse liver cells. Activation of the receptor activates adenylyl cyclase, which ultimately leads to the activation of PKA, which then phosphorylates a transcription factor called TFS on threonine 42. This phosphorylation is necessary for the binding of TFS to its specific sites on DNA, where it then activates the transcription of Sze2, a gene that encodes a protein important for liver cell growth. You find that liver cells lacking the receptor are 15% smaller than normal cells, whereas cells that express a constitutively activated version of PKA are 15% larger than normal liver cells. Given these results, predict whether you would expect the cell's size to be bigger or smaller than normal cells if cells were treated in the following fashion D. You add a drug that increases the activity of cyclic AMP phosphodiesterase.
Smaller
You are interested in cell-size regulation and discover that signaling through an enzyme-coupled receptor is important for the growth (enlargement) of mouse liver cells. Activation of the receptor activates adenylyl cyclase, which ultimately leads to the activation of PKA, which then phosphorylates a transcription factor called TFS on threonine 42. This phosphorylation is necessary for the binding of TFS to its specific sites on DNA, where it then activates the transcription of Sze2, a gene that encodes a protein important for liver cell growth. You find that liver cells lacking the receptor are 15% smaller than normal cells, whereas cells that express a constitutively activated version of PKA are 15% larger than normal liver cells. Given these results, predict whether you would expect the cell's size to be bigger or smaller than normal cells if cells were treated in the following fashion. A. You change threonine 42 on TFS to an alanine residue.
Smaller
A protein kinase can act as an integrating device in signaling if it ___________________. (a) phosphorylates more than one substrate. (b) catalyzes its own phosphorylation. (c) is activated by two or more proteins in different signaling pathways. (d) initiates a phosphorylation cascade involving two or more protein kinases.
c
Acetylcholine is a signaling molecule that elicits responses from heart muscle cells, salivary gland cells, and skeletal muscle cells. Which of the following statements is false? (a) Heart muscle cells decrease their rate and force of contraction when they receive acetylcholine, whereas skeletal muscle cells contract. (b) Heart muscle cells, salivary gland cells, and skeletal muscle cells all express an acetylcholine receptor that belongs to the transmitter-gated ion channel family. (c) Active acetylcholine receptors on salivary gland cells and heart muscle cells activate different intracellular signaling pathways. (d) Heart muscle cells, salivary gland cells, and skeletal muscle cells all respond to acetylcholine within minutes of receiving the signal.
b
Cell lines A and B both survive in tissue culture containing serum but do not proliferate. Factor F is known to stimulate proliferation in cell line A. Cell line A produces a receptor protein (R) that cell line B does not produce. To test the role of receptor R, you introduce this receptor protein into cell line B, using recombinant DNA techniques. You then test all of your various cell lines in the presence of serum for their response to factor F, with the results summarized in Table Q16-1. Table Q16-1 Which of the following cannot be concluded from your results above? (a) Binding of factor F to its receptor is required for proliferation of cell line A. (b) Receptor R binds to factor F to induce cell proliferation in cell line A. (c) Cell line A expresses a receptor for factor F. (d) Factor F is not required for proliferation in cell line B.
b
During the mating process, yeast cells respond to pheromones secreted by other yeast cells. These pheromones bind GPCRs on the surface of the responding cell and lead to the activation of G proteins inside the cell. When a wild-type yeast cell senses the pheromone, its physiology changes in preparation for mating: the cell stops growing until it finds a mating partner. If yeast cells do not undergo the appropriate response after sensing a pheromone, they are considered sterile. Yeast cells that are defective in one or more components of the G protein have characteristic phenotypes in the absence and presence of the pheromone, which are listed in Table 16-34. Table Q16-34 Which of the following models is consistent with the data from the analysis of these mutants? Explain your answer. (a) α activates the mating response but is inhibited when bound to βγ (b) βγ activates the mating response but is inhibited when bound to α (c) the G protein is inactive; either free α or free βγ complex is capable of activating the mating response (d) the G protein is active; both free α and free βγ complex are required to inhibit the mating response
b
Figure Q16-18 shows the pathway through which nitric oxide (NO) triggers smooth muscle relaxation in a blood-vessel wall. Which of the following situations would lead to relaxation of the smooth muscle cells in the absence of acetylcholine? Figure Q16-18 (a) a smooth muscle cell that has a defect in guanylyl cyclase such that it cannot bind NO (b) a muscle cell that has a defect in guanylyl cyclase such that it constitutively converts GTP to cyclic GMP (c) a muscle cell that has cyclic GMP phosphodiesterase constitutively active (d) a drug that blocks an enzyme involved in the metabolic pathway from arginine to NO
b
Male cockroaches with mutations that strongly decrease the function of an RTK called RTKX are oblivious to the charms of their female comrades. This particular RTK binds to a small molecule secreted by sexually mature females. Most males carrying loss-of-function mutations in the gene for Ras protein are also unable to respond to females. You have just read a paper in which the authors describe how they have screened cockroaches that are mutant in RTKX for additional mutations that partly restore the ability of males to respond to females. These mutations decrease the function of a protein that the authors call Z. Which of the following types of protein could Z be? Explain your answer. (a) a protein that activates the Ras protein by causing Ras to exchange GDP for GTP (b) a protein that stimulates hydrolysis of GTP by the Ras protein (c) an adaptor protein that mediates the binding of the RTKX to the Ras protein (d) a transcriptional regulator required for the expression of the Ras gene
b
The activation of the serine/threonine protein kinase Akt requires phosphoinositide 3-kinase (PI 3-kinase) to _________. (a) activate the RTK. (b) create phosphorylated lipids that serve as docking sites that localize Akt to the plasma membrane. (c) directly phosphorylate Akt. (d) to create DAG
b
The growth factor RGF stimulates proliferation of cultured rat cells. The receptor that binds RGF is a receptor tyrosine kinase called RGFR. Which of the following types of alteration would be most likely to prevent receptor dimerization? (a) a mutation that increases the affinity of RGFR for RGF (b) a mutation that prevents RGFR from binding to RGF (c) changing the tyrosines that are normally phosphorylated on RGFR dimerization to alanines (d) changing the tyrosines that are normally phosphorylated on RGFR dimerization to glutamic acid
b
The last common ancestor to plants and animals was a unicellular eukaryote. Thus, it is thought that multicellularity and the attendant demands for cell communication arose independently in these two lineages. This evolutionary viewpoint accounts nicely for the vastly different mechanisms that plants and animals use for cell communication. Fungi use signaling mechanisms and components that are very similar to those used in animals. Which of the phylogenetic trees shown in Figure Q16-59 does this observation support?
b
When a signal needs to be sent to most cells throughout a multicellular organism, the signal most suited for this is a ___________. (a) neurotransmitter. (b) hormone. (c) dissolved gas. (d) scaffold.
b
