Chapter 22 - Genomics 1
What are the steps involved in fluorescent in situ hybridization (FISH)?
1. Treat cells with agents that cause them to swell and fix them onto a slide 2. Denature chromosomal DNA 3. Hybridize chromosomal DNA to single-stranded DNA probes containing biotin 4. Add fluorescently labeled avidin 5. View with a fluorescene microscope
What is a Microsatellites?
A short DNA sequence repeated many times in a row
In a FISH experiment, what molecule is incorporated into the single-stranded DNA probes that later allows for detection of the probes?
Biotin
How can eukaryotic chromosomes be distinguished from one another in the microscope?
Chromosomes can be treated with dyes that produce unique banding patterns for each chromosome
What process is used for physical mapping?
Cloning fragments of chromosomal DNA
What is the goal of cytogenetic mapping?
To localize a gene to a site within a chromosomal banding pattern
Cytogenetics can distinguish one chromosome from another based on the ... patterns that are obtained when chromosomes are treated with certain dyes
banding
A collection of clones containing overlapping pieces of chromosomal DNA that can be organized into a physical map is called a ...
contig
The total genetic composition of an organism or species is its ...
genome
A technique called ... hybridization can be used to localize a gene of interest due to the ability of a cloned DNA sequence to hybridize to a chromosome through complementary base pairing
in situ
If a geneticist states that a polymorphic marker is linked to a disease-causing allele, he or she means that the marker ...
is close to the disease-causing allele
During in situ hybridization, a DNA probe made from a cloned gene binds to ...
its complementary sequence on a particular chromosome
A collection of recombinant vectors that each contain a particular fragment of chromosomal DNA is called a DNA ...
library
The mapping technique that relies on the frequency of recombinant offspring for determination of the distance between genetic loci is ... mapping
linkage
In genetics, the experimental process of determine the relative locations of genes or other DNA segments along individual chromosomes is referred to as ...
mapping
Molecular markers can be used to identify the location of a disease-causing gene because they ...
may be located close to a disease-causing gene
The area of study known as ... is focused on determine the roles of all cellular proteins
proteomics
In linkage mapping, the distance between sites on the same chromosome is determined by calculating the frequency of ...
recombinant offspring
An organisms genome is its...
total genetic composition
A segment of DNA that is localized at a specific site along a chromosome and that has properties that allow it to be uniquely identified using molecular tools is called a ... marker
molecular
