Exam 1 (ABO & Rh)

What antigens are expressed on the surface of the rbc and what substances are expressed in the body fluids if the individual has inherited the following genes: OO, HH, Sese? a. rbc surface = H; body fluids = H b. rbc surface = H; body fluids = None c. rbc surface = None; body fluids = H d. rbc surface = None; body fluids = None

rbc surface = H; body fluids = H

In the Rh system, which antigen is the most antigenic? a. D b. C c. E d. c e. e

D

What is the pts. blood type? A:4 B:0 a:0 b:4 D:4 C:0 a. AB pos b. AB neg c. O pos d. O neg e. A pos f. A neg g. B pos h. B neg i. discrepancy

A pos

If a patient antigen typed with the following reactions, which one of the possibilities listed would be consistent with the Rosenfield nomenclature: D:0 C:+ c:+ E:0 e:+ a. 1, 2, -3, 4, -5 b. -1, 2, -3, 4, 5 c. -1, 2, 3, -4, 5 d. 1, 2, 3, -4, -5 e. -1, -2, 3, 4, 5

-1, 2, -3, 4, 5

According to the International Society of Blood Transfusion (ISBT), the Rh system is assigned which of the following 3 digit codes? a. 001 b. 002 c. 003 d. 004 e. 005

004

On which chromosome is the RH Locus located? a. 1 b. 3 c. 6 d. 9

1

If the Father's type (phenotype) was AB and the Mother's type was A, what would be the possible genotypes of their offspring? (Include A2 subgroups). a. A1B, A1A1, A2B, A2O, BB, BO b. A1B, A2B, A1A2, A1O, A2O, BO, OO c. A1B, A2B, A1A2, A2A2, BO, A1O, A2O, OO d. A1B, A2B, A1A1, A1A2, A1O, A2O, A2A2, BO

A1B, A2B, A1A1, A1A2, A1O, A2O, A2A2, BO

Which one of the following is considered recessive? a. A1 b. A2 c. c d. Se

A2

Which of the following would be considered Codominant? a. AO b. AB c. A1A2 d. Dd

AB

What is the pts. blood type? A: 3 B: 3 a: 0 b: 0 D: 2 C:0 a. AB pos b. AB neg c. O pos d. O neg e. A pos f. A neg g. B pos h. B neg i. discrepancy

AB pos

If the Father's type (phenotype) was AB and the Mother's type was B, what would be the possible genotypes of their offspring? (Not including any subgroups). a. AA, AO, BB, BO b. AB, AA, BB, AO c. AB, AO, BB, BO d. AO, AA, BO, OO

AB, AO, BB, BO

All of the following (a-d) would be classified as natural occurring antibodies (produced from "look alike antigens" in the environment and not from foreign red blood cell exposure) except: a. Anti-A b. Anti-B c. Anti-A,B d. Anti-D e. All of the above (a-d) would be classified as natural occurring antibodies

Anti-D

Which type A subgroup could be mistyped as Type O because it only has ~4000 A antigen sites per RBC a. A1 b. A2 c. A3 d. Ax

Ax

What is the pts. blood type? A: 0 B:4 a:2 b:0 D:0 C:0 a. AB pos b. AB neg c. O pos d. O neg e. A pos f. A neg g. B pos h. B neg i. discrepancy

B neg

Weak D Testing is required to be performed on patients having this positional effect which causes decreased reactivity with Anti-D: a. Genetic Weak D b. C in Trans to RHD c. Partial D (D Mosiac) d. Del Phenotype

C in Trans to RHD

Which would be considered a heterozygous expression? a. OO b. ee c. cE d. Cc

Cc

Which of the following Rh antigen scenarios (reaction strengths) correctly demonstrates dosage? a. DD (4+); Dd (2+) b. CC (1+); Cc (3+) c. Cc (1+); cc (2+) d. EE (2+); Ee (2+)

Cc (1+); cc (2+)

Which of the following alleles (a-d) would not be considered Codominant? a. Dd b. Cc c. Ee d. AB e. All of the above alleles (a-d) would be considered Codominant.

Dd

What is the name of the lectin for anti-A1? Dolichos biflrous Glycine soja Salvia sclarea Ulex europaeus

Dolichos biflrous

Which antigenic determinant appears on both D and C antigen? a. Cw b. f c. G d. V

G

A, B, and H antigens on the red blood cell surface are: a. Aminoproteins b. Glycoproteins c. Phospholipids d. Glycolipids

Glycolipids

What antibody class are ABO antibodies primarily? a. IgA b. IgE c. IgG d. IgM

IgM

The first step in resolving an ABO discrepancy is: a. Change reagents b. Repeat the testing c. Wash the patient's rbcs d. Have a new specimen drawn from the patient

Repeat the testing

Which of the following techniques would be used to resolve or verify the ABO discrepancy below: A:4 B:0 a:0 b:0 D:4 C:0 AC:0 a. Wash patient RBCs several times and then repeat front and reverse typing b. Pre-warm patient RBCs and reagent red cells (A1/B) at 37°C for 15-30 minutes and then repeat front and reverse typing c. Incubate Reverse Type at Room Temperature for 15-30 minutes and then repeat reverse typing d. Test patient RBCs with Anti-A1 Lectin e. Pretreat patient RBCs with appropriate enzymes or acetic anhydride and then repeat front and reverse typing

Incubate Reverse Type at Room Temperature for 15-30 minutes and then repeat reverse typing

What is the discrepancy in the blood typing? A:4 B: a:0 b:0 D:4 C:0 AC:0 a. Missing antibody b. Extra antigen c. Extra non-ABO group antibody d. Subgroup

Missing antibody

The immunodominant sugar responsible for blood group A specificity is: a. L-fucose b. N-acetyl D galactosamine c. D-galactose d. A-Lactose

N-acetyl D galactosamine

If you gave a donor unit that was dce/dce to a transfusion recipient that was DCE/Dce, would there be a possibility of an unexpected Rh antibody being produced? a. Yes --- Anti-D b. Yes --- Anti-D & Anti-C c. Yes --- Anti-D, Anti-C & Anti-E d. Yes --- Anti-C & Anti-E e. No

No

What is the pts. blood type? A:0 B:0 a:3 b:3 D:0 C:0 a. AB pos b. AB neg c. O pos d. O neg e. A pos f. A neg g. B pos h. B neg i. discrepancy

O neg

According to AABB Standards, Weak D Testing is required to be performed in all of the following situations (a-d) except: a. Pre-transfusion testing on a patient with a past history of Anti-D b. Obstetrical cases involving Rh Negative mothers c. Donor unit testing d. Pre-transfusion testing for both males and females of reproductive age e. All of the above situations (a-d) require Weak D Testing per AABB Standards

Pre-transfusion testing for both males and females of reproductive age

What would the secretor status results look like for a patient that was BB, sese? a. Saliva + anti-A (+) a cells = Agglutination Saliva + anti-B (+) b cells = Agglutination Saliva + anti-H (+) O cells = Agglutination b. Saliva + anti-A (+) a cells = No Agglutination Saliva + anti-B (+) b cells = Agglutination Saliva + anti-H (+) O cells = No Agglutination c. Saliva + anti-A (+) a cells = Agglutination Saliva + anti-B (+) b cells = No Agglutination Saliva + anti-H (+) O cells = No Agglutination d. Saliva + anti-A (+) a cells = Agglutination Saliva + anti-B (+) b cells = Agglutination Saliva + anti-H (+) O cells = No Agglutination

Saliva + anti-A (+) a cells = Agglutination Saliva + anti-B (+) b cells = Agglutination Saliva + anti-H (+) O cells = Agglutination

3+ agglutination appears as: a. One large solid agglutinate with a clear background b. Very fine agglutinates with a red background c. Several large clumps with a clear background d. Several small-medium sized agglutinates with a clear background e. Complete hemolysis is seen (with no agglutinates)

Several large clumps with a clear background

All of the following characteristics are true of the Bombay Phenotype except: a. hh (sese) b. No H antigen on rbcs c. Types as Group O with routine ABO Front & Reverse Typing d. Should be given O negative donor units if a transfusion is needed e. Serum contains anti-A, anti-B, anti-A,B and a potent-wide thermal range anti-H

Should be given O negative donor units if a transfusion is needed

What is the discrepancy in the blood typing? a. Missing antibody b. Extra antigen c. Extra non-ABO group antibody d. Subgroup

Subgroup

What is the discrepancy in the blood typing? A:4 B:0 a:2 b:3 D:4 C:0 AC:0 a. Wash patient RBCs several times and then repeat front and reverse typing b. Pre-warm patient RBCs and reagent red cells (A1/B) at 37°C for 15-30 minutes and then repeat front and reverse typing c. Incubate Reverse Type at Room Temperature for 15-30 minutes and then repeat reverse typing d. Test patient RBCs with Anti-A1 Lectin e. Pretreat patient RBCs with appropriate enzymes or acetic anhydride and then repeat front and reverse typing

Test patient RBCs with Anti-A1 Lectin

All of the following are true of ABO antibodies except: a. Type A1 adults produce naturally occurring, broad thermal range IgM Anti-B b. Type A2 adults produce naturally occurring, broad thermal range IgM Anti-B c. Type AB adults produce naturally occurring, broad thermal range IgM Anti-A and Anti-B d. Type O adults produce a naturally occurring, IgG Anti-A, Anti-B, and Anti-A,B; as well as, broad thermal range IgM Anti-A and Anti-B e. Newborns should not be typed for naturally occurring ABO antibodies

Type AB adults produce naturally occurring, broad thermal range IgM Anti-A and Anti-B

Which blood group has the highest concentration of H antigen? a. Type A b. Type B c. Type A1B d. Type A2B e. Type O

Type O

1+ agglutination appears as: a. One large solid agglutinate with a clear background b. Very fine agglutinates with a red background c. Several large clumps with a clear background d. Several small-medium sized agglutinates with a clear background e. Complete hemolysis is seen (with no agglutinates)

Very fine agglutinates with a red background

What is the f antigen? a. ce in cis position b. Ce in trans position c. CE in cis position d. DC in trans position

ce in cis position

If a patient antigen typed with the following reactions, which one of the possibilities listed would be consistent with the Fisher-Race nomenclature: D0 C0 c+ E0 e+ a. dce/dce b. Dce/dce c. dCE/Dce d. DCe/dcE e. DCE/dce

dce/dce

Which of the following would be considered Rh negative? a. 1, 2, 3, 4, -5 b. dd c. Dd d. DD

dd

What is the pts. blood type? A: 4 B:0 a:0 b:2 D:0 C:2 a. AB pos b. AB neg c. O pos d. O neg e. A pos f. A neg g. B pos h. B neg i. discrepancy

discrepancy

What is the pts. blood type? A: 4 B:0 a:4 b:0 D:0 C:0 . AB pos b. AB neg c. O pos d. O neg e. A pos f. A neg g. B pos h. B neg i. discrepancy

discrepancy

Which of the following shows ABO frequencies from most common to least common? a. A > O > AB > B b. AB > A > B > O c. B > AB > A > O d. A > B > AB > O e. O > A > B > AB

e. O > A > B > AB

If a patient is Type B, what two transferases must they have inherited to build the B antigen out of the precursor molecule? a. α-2-L-fucosyltransferase & α-3-N-acetylgalactosaminyl-transferase b. α-2-L-fucosyltransferase & α-3-D-galactosyltransferase c. α-3-N-acetylgalactosaminyl-transferase & α-3-D-galactosyltransferase d. hh-transferase & α-2-L-fucosyltransferase e. hh-transferase & α-3-D-galactosyltransferase

α-2-L-fucosyltransferase & α-3-D-galactosyltransferase

What specific glycosyltransferase is required to make an H antigen --- and what immunodominant sugar does the transferase add to the precursor molecule? a. α-3-N-acetylgalactosaminyl-transferase --- N-acetyl-D-galactosamine b. α-2-L-fucosyltransferase --- L-fucose c. α-3-D-galactosyltransferase --- D-galactose d. hh-transferase --- Bombay sugar

α-2-L-fucosyltransferase --- L-fucose