Forensic Bio Final
Confirmatory assays for saliva:
Immunochromatographic ELISA RNA-based
Current method for semen
Immunochromatographic assay
Species Identification Assays
Immunochromatographic, Precipitation-based, Ring, Ouchterlony, Crossed-Over Electrophoresis
Two groups of repetitive DNA
Interspersed elements and Tandem arrays
Phases of the Uterine cycle
Menstrual, Proliferative, Secretory
Semen: Confirmatory tests
Microscopy for spermatozoa, Antigen-antibody interactions
Menstruation
monthly shedding of the uterine lining
Aspermia
no sperm
Alleles
difference in number of repeats (any variation of a locus)
Amylase
digests starch in the oral cavity and small intestine
Two sweat glands
eccrine and apocrine
Urinary excretory system
eliminates soluble toxic wastes that are cellular metabolic by-products
Beta amylase
plant and bacterial sources
Urea
results from the elimination of ammonia that is produced from the metabolic process of amino acids
Seminal vesicle specific antigens (SVSA)
higher concentration and greater specificity than PSA
A Amylase HPA
human pancreas
A Amylase HSA
human saliva
Urinary system
kidney, ureter, urinary bladder, urethra
Oligospermia
low sperm count
Most commonly used blood group system
ABO
The analysis of VNTR alleles in forensic DNA profile analysis is based on what common method of molecular biology?
Southern hybridization
How many different alleles can be detected per heterozygous individual with a specific single-locus probe in DNA fingerprint analysis?
2. Two different alleles can be detected, one of each copy of the chromosome of interest. One allele is inherited from mother and one from father. If the maternal and paternal alleles are the same, only one allele will be detected.
Typical ejaculation
2.5 to 5 mL , 160 million sperm
Prostate Specific Antigen (PSA)
3 years half-life; major protein in seminal fluid
Average urea concentration
9 g/L
Two types of amylase
Beta (B) and Alpha (A)
Presumptive assays for saliva:
Colorimetric assays Phadebas Test Start-iodine assays Amylase overlay assay Amylase radial diffusion assay
Semen: Presumptive tests
Colorimetric, Fluorimetric
The vaginal acid phosphatase has has higher electrophoretic mobility toward an anode (a positively charged electrode)
False
Spermatozoa
Head (acrosome and nucleus with haploid DNA), Middle (mitochondria), Tail (flagella; mobility)
Species Identification Assays: Immunochromotographic assays
Hexagon OBTI, ABAcard HemaTrace (hemoglobin), RSID-Blood (glycophorin A)
Hemostasis
Occurs at the site of injury of a blood vessel
Which of the following is NOT a part of the methods used in single locus probe analysis of VNTR regions of human DNA?
Recombinant DNA
Titer
The reciprocal of the highest dilution giving a positive reaction
Minisatellite
an array of tandem repeats
Microsatellite
an array of very short repeats
The prostate acid phosphatase has higher electrophoretic mobility toward an anode (a positively charged electrode)
True
predominant bacteria in vagina
Vaginal Lactobacillus bacteria
Urine
an aqueous solution consisting of water and urea
Vasectomy
block spermatozoa
Sperm cells
spermatozoa
Nephron
the basic structural and functional unit of the kidney (where formation of urine takes place)
How do the many VNTR alleles at a single locus differ from each other?
the number of times a simple DNA sequence is repeated