Genetics CH 9

mixed coat proteins of one strain of TMV with RNA from another TMV strain and determining which strain's phenotype was seen

An experiment was performed by Frankel-Conrat and workers to demonstrate that Tobacco Mosaic Virus (TMV) uses RNA rather than DNA for its genetic material. What did they do?

treat extracts of type IIIS with different enzymes to look for loss of transforming factor

How is it demonstrated that DNA is the "transforming principle"?

labeled sulfur

By labeling T2 phage with radioactive isotopes, Hershey and Chase were able to tell that DNA was the genetic material when __________ was found in the cells of test organisms.

146

Each nucleosome

27

For a given DNA sample, you calculate it has 23% adenine. What is the percentage of cytosine?

isotopes of phosphorus and sulfur

Hershey and Chase used _________ to determine that DNA is definitively the storage material for genetic information.

X ray chromotography

Many people think that Rosalind Franklin, had she not died prior to 1962, should have shared in the Nobel Prize that Watson and Crick received. What data did Rosalind Franklin collect that was essential for understanding the nature and structure of DNA?

TAG

One of the strands in DNA has a sequence: 5' ATCTGCTACGA-3'. A complementary DNA sequence to this strand should be:

Yes, if you incubate the dead IIIS cells with live IIR cells, you will recover live IIIS cells through the process of transformation.

Suppose a lab mate accidentally sets the incubator to 73° instead of 37°, killing your valuable culture of Type IIIS Streptococcus cells. Can these cells be recovered?

precipitate the IIR cells with serum and add Dnase

Suppose the same lab mate, accidentally spilled a tube containing Type IIIS DNA into a culture of IIR cells. How could you ensure that the culture of your nonpathogenic Streptococcus cells does not become pathogenic?

allow for the replication of the linear DNA molecules without loss of genetic material

Telomeres are repetitive DNA sequences with unique properties that function to:

complementarity

The _________ of the DNA double helix makes DNA uniquely suited to store and transmit genetic information from one generation to the next,

H1

The complete chromatin subunit is stabilized by the binding of one molecule of __________ to the outside of the structure.

hydrogen bonds

The stability of the DNA double helix is the result of:

a left handed structure

There are several alternate forms of double helical DNA. The Z DNA form is:

to condense the bacterial genome so it will fit into the cell

What is the function of negative supercoiling bacteria?

regulating gene expression

What is the function of nonhistone chromosomal proteins in human chromosomes?

50% of the bases are purines

Which of the following is true about double stranded DNA?

phosphodiester bond

Which type of bond is formed between two nucleotides in a polynucleotide chain?

label the phage T2 with 32 P infect the cells, and look for no radioactivity in the cell pellet

You have a strain of E. coli that seems to be resistant to transfection by Phage T2. How can you test your theory?

supercoils

___________ allow DNA to be collapsed into a tight structure.

B-DNA

______________ is the conformation that DNA takes under physiological conditions.