Genetics Test 3

If you have a 200 ul sample of double-stranded DNA. The A260 of 1 ul=0.6. How many micrograms of DNA do you have?

(0.6 x 50)=3 = 30ng x 200 = 6000 / 1000 = 6.0

DNA and RNA differ in the OH number on what carbon

2'

DNA and RNA differ in the -OH number on their

2' C

You have a 100 ul sample o double-stranded RNA. The₂₆₀ of 1 ul=.5. How many micrograms of RNA do you have?

2.0

What is the complemenatary DNA sequence to 5' ATGCTTGACTG 3'

3' TACGAACTGAC 5'

Which of the following DNA fragments would be located third down from the beginning (negative terminal) of the gel? 3, 10, 1, 5, 0.5

3kb

If 10% of the nitrogenous bases in a sample of DNA from a particular organism is guanosine, what percentage should be adenosine?

40%

RNA

40ng/μl/A260

Single-stranded DNA

=33ng/μl/A260

Double-stranded DNA

=50ng/μl/A260

The secondary structure of a protein includes ________. A) gamma and delta B) alpha and gamma C) α-helix and β-pleated sheet D) hydrophobic clusters E) disulfide bridges

C

In the Meselson-Stahl experiment, which mode of replication was eliminated based on data derived after one generation of replication using double-stranded DNA?

Conservative

In the Meselson-Stahl experiment, which replication was eliminated?

Conservative

Best describe the structure of a DNA molecule

DNA is composed of a sugar-phosphate backbone with bases projecting toward the inside of the backbone

What statement best describes the structure of a DNA molecule?

DNA is composed of a sugar-phosphate backbone with bases projecting toward the inside of the backbone.

After removal of RNA primers, Okazaki fragments are joined through a reaction that involves which one of the following enzymes

DNA ligase

After removal of the RNA primers, Okazaki fragments are joined through a reaction that involves which of the following enzymes

DNA ligase

The telomeric repeat is TTGGGG (strand A) AACCCC (strand b).Which strand with the DNA polymerase use as a template for the leading strand?

DNA polymerase will use the B strand

What results did Avery, McLeod and McCarty obtain in their experiments with virulent bacteria

DNase destroyed the transforming activity

In the Meselson-Stahl experiment, which mode of replication was eliminated based on data derived after melting the two strands of DNA apart and running single stranded DNA through the CsCl gradient?

Dispersive

In mammalian cells, which DNA polymerase corresponds to DNA polymerase III on the lagging strand of prokaryotes?

Epsilon

Avery, McLeod and McCarty and Hershey-Chase determined that DNA is the genetic material in eukaryotes.

False

Bean plants (vicia faba) were used to show semi-conservative replication in prokaryotes.

False

Bean plants were used to show semi-conservative replication

False

Griffith discovered DNA as the transforming principle

False

Griffith discovered DNA as the transforming principle.

False

Hershey and Chase used labeled DNA and protein to determine that DNA is the genetic material in bacteria. T/F

False

In RNA, there is one -OH group less on the sugar than DNA

False

In the Hershey-Chase experiment, DNA was labeled with 35S and protein with 32P.

False

The final step of the proof of DNA as the genetic material in the Avery, McLeod, and McCarty experiment was the RNase treatment of the filtrate and transformation of type IIR cells into type IIIS cells

False

When considering the structure of RNA, we would say that the two strands are anti-parallel.

False

When considering the structure of RNA, we would say that the two strands are antiparallel

False

When considering the structure of DNA, we would say that the complementary strands are parallel. T/F

False (antiparallel)

In RNA, there is one -OH group less on the sugar than in DNA

False, DNA has one less -OH group

Avery, McLeod and McCarty and Hershey-Chase determined that DNA is the genetic material in eukaryotes

False-they discovered it in prokaryotes

What enzyme fucntions to deal with supercoils?

Gyrase

What enzyme unwinds the DNA

Helicase

Nucleic acids were shown to be the genetic material by

Hershey and Chase and Avery, Mcleod, and McCarthy

Nucleic acids were shown to e the genetic material by:

Hershey and Chase, and Avery, McLeod, and McCarthy

What enzyme couples two newly synthesized fragments of DNA

Ligase

Which nucleotide sequence combo accurately depicts the situation upon completion of the second step of the telomerase synthesis of chromosomal ends?

Line up at the end, top strand is much longer

What is used to study global gene expression?

Microchips

What conclusion could be drawn if Hershey and Chase had found only 35S in their bacterial cells?

Protein is the genetic material

What nucleic acid is represented by the dots?

RNA

Experiements with the Tobacco Mosaic Virus showed

RNA is the transforming molecule in some cases

Experiments with the Tobacco Mosaic Virus showed...

RNA is the transforming molecule in some cases

Northerns

RNA size and gene expression

Why is RNA primer considered essential for DNA synthesis by DNA polymerase III

The enzyme requires a free 3'-OH group

Why is an RNA primer considered essential for DNA synthesis by DNA polymerase III?

The enzyme requires a free 3'-OH group.

What observation did Griffith make in his experiments with Steptococcus pneumoniae?

The mouse did not survive when injected with a mixture of live, avirulent (rough) Streptococcus pneumoniae and heat-killed, virulent Strep. pneumoniae.

What observation did Griffith make in his experiments with strep pneumonia

The mouse did not survive with a mix of live, avirulent strep and heat-killed virulent strep

Which nucleotide sequence combo accurately depicts the situation upon completion of the first step of the telomerase synthesis of chromosomal ends?

The one were they line up at the end

Which depiction of the end of a double stranded DNA after synthesis of the chromosome is completed and before the telomerase mechanism best represnts the need for this special mechanism

The one with a bunch hanging off the top strand

DNA replication occurs in the 5' to 3' direction, that is, new nucleotides are added to the 3' end. T/F

True

Endonucleases are highly specific in the DNA sequence they recognize.

True

G and C are present in both DNA and RNA. T/F

True

The alleles that we used in the Molecular Genotyping lab exercise were from repeated regions of DNA called microsatellites. On a CoT curve, these are called HR regions. T/F

True

The base content of a DNA sample is found to be: A=31%, G=31%, T=19%, C=19%. The DNA in this sample is single stranded. T/F

True

The four characteristics of a hereditary molecule are: it must replicate accurately, it must be capable of change, it must store information, and it must be capable of expression

True

The unique piece of information a Northern provides compared to other RNA analysis techniques is that it tells one the seize of RNA

True

The unique piece of information a Northern provides compared to other RNA analysis techniques is that it tells one the size of the RNA.

True

Thymine is a pyrimidine base. T/F

True

What is used to store all the genetic information from an organism for study?

a library

In mammalian cells, which DNA polymerase corresponds to the primase in prokaryotes?

alpha

Bacteria containing plasmids with DNA inserts are forced to maintain this DNA because of

an antibiotic resistance gene on the plasmids conferring resistance to antibiotics

In the tobacco mosaic virus study, the purpose of the reconstituted hybrid virus allowed one to

ask whether DNA or protein directed synthesis of the new virus

In the tobacco mosaic virus (TMV)/holmes ribgrass (HR) virus study, the purpose of the reconstituted hybrid virus allows one to

ask whether RNA or protein directed synthesis of the new virus

Which terms accurately reflect the nature of DNA replication in prokaryotes?

bidirectional, semiconservative

Which set of terms accurately reflects the nature of DNA replication in prokaryotes?

bidirectional; semi conservative

In Taylor, Woods, Hughs experiment, conservative replication would have been shown if:

both strands of the DNA in a single chromatid of a chromosome were labeled.

Microchip DNA analysis gives teh advantage over other RNA analysis techniques of

global gene expression

Microchip DNA analysis gives the advantage over other RNA analysis techniques of:

global gene expression

The four characteristics of a hereditary molecule are..

it must replicate accurately it must change it must store info must be capable o expression

Regarding the structure of DNA, the covalently arranged combination of a deoxyribose, a nitrogenous base, and the phosphate group would be called a

nucleotide

Regarding the structure of DNA, the covalently arranged combination of a deoxyribose, a nitrogenous base, and the phosphate group would be called a:

nucleotide

Southerns

one purpose, look at gene copy #

What conclusion could be drown if Hershey and Chase had found only ³⁵S in their bacterial cells

protein is the genetic material

The telomeric repeat is TTGGGG (strand A) AACCCC (strand b) .Which strand will the telomerase use as a template for the leading strand?

telomerase will use A strand

When DNA polymerase I backfills the former RNA primer with DNA it uses

the 3' C of the DNA of the previous Okazaki fragment to prime the reaction

When DNA polymerase I backfills the former RNA primer with DNA it uses:

the 3' C of the DNA of the previous Okazaki fragment to prime the reaction

DNA polymerase III adds nucleotides to..

the 3' end of the RNA primer

The discontinous aspect of replication of DNA in a cell is caused by

the 5' - 3' directional basis of replication

The discontinuous aspect (the need of a leading and lagging strand) of replication of DNA in the cell is caused by:

the 5' to 3' directional basis of replication

The key to sequencing with ddNTP's is the removal of

the removal of the 3' -OH

DNA polymerase III adds nucleotides...

to the 3' end of the RNA primer