Genetics Test 3
If you have a 200 ul sample of double-stranded DNA. The A260 of 1 ul=0.6. How many micrograms of DNA do you have?
(0.6 x 50)=3 = 30ng x 200 = 6000 / 1000 = 6.0
DNA and RNA differ in the OH number on what carbon
2'
DNA and RNA differ in the -OH number on their
2' C
You have a 100 ul sample o double-stranded RNA. The₂₆₀ of 1 ul=.5. How many micrograms of RNA do you have?
2.0
What is the complemenatary DNA sequence to 5' ATGCTTGACTG 3'
3' TACGAACTGAC 5'
Which of the following DNA fragments would be located third down from the beginning (negative terminal) of the gel? 3, 10, 1, 5, 0.5
3kb
If 10% of the nitrogenous bases in a sample of DNA from a particular organism is guanosine, what percentage should be adenosine?
40%
RNA
40ng/μl/A260
Single-stranded DNA
=33ng/μl/A260
Double-stranded DNA
=50ng/μl/A260
The secondary structure of a protein includes ________. A) gamma and delta B) alpha and gamma C) α-helix and β-pleated sheet D) hydrophobic clusters E) disulfide bridges
C
In the Meselson-Stahl experiment, which mode of replication was eliminated based on data derived after one generation of replication using double-stranded DNA?
Conservative
In the Meselson-Stahl experiment, which replication was eliminated?
Conservative
Best describe the structure of a DNA molecule
DNA is composed of a sugar-phosphate backbone with bases projecting toward the inside of the backbone
What statement best describes the structure of a DNA molecule?
DNA is composed of a sugar-phosphate backbone with bases projecting toward the inside of the backbone.
After removal of RNA primers, Okazaki fragments are joined through a reaction that involves which one of the following enzymes
DNA ligase
After removal of the RNA primers, Okazaki fragments are joined through a reaction that involves which of the following enzymes
DNA ligase
The telomeric repeat is TTGGGG (strand A) AACCCC (strand b).Which strand with the DNA polymerase use as a template for the leading strand?
DNA polymerase will use the B strand
What results did Avery, McLeod and McCarty obtain in their experiments with virulent bacteria
DNase destroyed the transforming activity
In the Meselson-Stahl experiment, which mode of replication was eliminated based on data derived after melting the two strands of DNA apart and running single stranded DNA through the CsCl gradient?
Dispersive
In mammalian cells, which DNA polymerase corresponds to DNA polymerase III on the lagging strand of prokaryotes?
Epsilon
Avery, McLeod and McCarty and Hershey-Chase determined that DNA is the genetic material in eukaryotes.
False
Bean plants (vicia faba) were used to show semi-conservative replication in prokaryotes.
False
Bean plants were used to show semi-conservative replication
False
Griffith discovered DNA as the transforming principle
False
Griffith discovered DNA as the transforming principle.
False
Hershey and Chase used labeled DNA and protein to determine that DNA is the genetic material in bacteria. T/F
False
In RNA, there is one -OH group less on the sugar than DNA
False
In the Hershey-Chase experiment, DNA was labeled with 35S and protein with 32P.
False
The final step of the proof of DNA as the genetic material in the Avery, McLeod, and McCarty experiment was the RNase treatment of the filtrate and transformation of type IIR cells into type IIIS cells
False
When considering the structure of RNA, we would say that the two strands are anti-parallel.
False
When considering the structure of RNA, we would say that the two strands are antiparallel
False
When considering the structure of DNA, we would say that the complementary strands are parallel. T/F
False (antiparallel)
In RNA, there is one -OH group less on the sugar than in DNA
False, DNA has one less -OH group
Avery, McLeod and McCarty and Hershey-Chase determined that DNA is the genetic material in eukaryotes
False-they discovered it in prokaryotes
What enzyme fucntions to deal with supercoils?
Gyrase
What enzyme unwinds the DNA
Helicase
Nucleic acids were shown to be the genetic material by
Hershey and Chase and Avery, Mcleod, and McCarthy
Nucleic acids were shown to e the genetic material by:
Hershey and Chase, and Avery, McLeod, and McCarthy
What enzyme couples two newly synthesized fragments of DNA
Ligase
Which nucleotide sequence combo accurately depicts the situation upon completion of the second step of the telomerase synthesis of chromosomal ends?
Line up at the end, top strand is much longer
What is used to study global gene expression?
Microchips
What conclusion could be drawn if Hershey and Chase had found only 35S in their bacterial cells?
Protein is the genetic material
What nucleic acid is represented by the dots?
RNA
Experiements with the Tobacco Mosaic Virus showed
RNA is the transforming molecule in some cases
Experiments with the Tobacco Mosaic Virus showed...
RNA is the transforming molecule in some cases
Northerns
RNA size and gene expression
Why is RNA primer considered essential for DNA synthesis by DNA polymerase III
The enzyme requires a free 3'-OH group
Why is an RNA primer considered essential for DNA synthesis by DNA polymerase III?
The enzyme requires a free 3'-OH group.
What observation did Griffith make in his experiments with Steptococcus pneumoniae?
The mouse did not survive when injected with a mixture of live, avirulent (rough) Streptococcus pneumoniae and heat-killed, virulent Strep. pneumoniae.
What observation did Griffith make in his experiments with strep pneumonia
The mouse did not survive with a mix of live, avirulent strep and heat-killed virulent strep
Which nucleotide sequence combo accurately depicts the situation upon completion of the first step of the telomerase synthesis of chromosomal ends?
The one were they line up at the end
Which depiction of the end of a double stranded DNA after synthesis of the chromosome is completed and before the telomerase mechanism best represnts the need for this special mechanism
The one with a bunch hanging off the top strand
DNA replication occurs in the 5' to 3' direction, that is, new nucleotides are added to the 3' end. T/F
True
Endonucleases are highly specific in the DNA sequence they recognize.
True
G and C are present in both DNA and RNA. T/F
True
The alleles that we used in the Molecular Genotyping lab exercise were from repeated regions of DNA called microsatellites. On a CoT curve, these are called HR regions. T/F
True
The base content of a DNA sample is found to be: A=31%, G=31%, T=19%, C=19%. The DNA in this sample is single stranded. T/F
True
The four characteristics of a hereditary molecule are: it must replicate accurately, it must be capable of change, it must store information, and it must be capable of expression
True
The unique piece of information a Northern provides compared to other RNA analysis techniques is that it tells one the seize of RNA
True
The unique piece of information a Northern provides compared to other RNA analysis techniques is that it tells one the size of the RNA.
True
Thymine is a pyrimidine base. T/F
True
What is used to store all the genetic information from an organism for study?
a library
In mammalian cells, which DNA polymerase corresponds to the primase in prokaryotes?
alpha
Bacteria containing plasmids with DNA inserts are forced to maintain this DNA because of
an antibiotic resistance gene on the plasmids conferring resistance to antibiotics
In the tobacco mosaic virus study, the purpose of the reconstituted hybrid virus allowed one to
ask whether DNA or protein directed synthesis of the new virus
In the tobacco mosaic virus (TMV)/holmes ribgrass (HR) virus study, the purpose of the reconstituted hybrid virus allows one to
ask whether RNA or protein directed synthesis of the new virus
Which terms accurately reflect the nature of DNA replication in prokaryotes?
bidirectional, semiconservative
Which set of terms accurately reflects the nature of DNA replication in prokaryotes?
bidirectional; semi conservative
In Taylor, Woods, Hughs experiment, conservative replication would have been shown if:
both strands of the DNA in a single chromatid of a chromosome were labeled.
Microchip DNA analysis gives teh advantage over other RNA analysis techniques of
global gene expression
Microchip DNA analysis gives the advantage over other RNA analysis techniques of:
global gene expression
The four characteristics of a hereditary molecule are..
it must replicate accurately it must change it must store info must be capable o expression
Regarding the structure of DNA, the covalently arranged combination of a deoxyribose, a nitrogenous base, and the phosphate group would be called a
nucleotide
Regarding the structure of DNA, the covalently arranged combination of a deoxyribose, a nitrogenous base, and the phosphate group would be called a:
nucleotide
Southerns
one purpose, look at gene copy #
What conclusion could be drown if Hershey and Chase had found only ³⁵S in their bacterial cells
protein is the genetic material
The telomeric repeat is TTGGGG (strand A) AACCCC (strand b) .Which strand will the telomerase use as a template for the leading strand?
telomerase will use A strand
When DNA polymerase I backfills the former RNA primer with DNA it uses
the 3' C of the DNA of the previous Okazaki fragment to prime the reaction
When DNA polymerase I backfills the former RNA primer with DNA it uses:
the 3' C of the DNA of the previous Okazaki fragment to prime the reaction
DNA polymerase III adds nucleotides to..
the 3' end of the RNA primer
The discontinous aspect of replication of DNA in a cell is caused by
the 5' - 3' directional basis of replication
The discontinuous aspect (the need of a leading and lagging strand) of replication of DNA in the cell is caused by:
the 5' to 3' directional basis of replication
The key to sequencing with ddNTP's is the removal of
the removal of the 3' -OH
DNA polymerase III adds nucleotides...
to the 3' end of the RNA primer
