Microbiology 440 (chapter 2)
A polysaccharide that acts as a solidifying agent in microbiological media is called ______________.
Agar
The polysaccharide found in a sea alga that is commonly used to prepare solid culture media is called ______. Peptone Starch Gelatin Agar
Agar
Which type(s) of organisms are usually observed with light microscopes? Viruses Prions Bacteria Arthropods
Bacteria
Media ingredients that cannot be chemically defined include which of the following? Multiple select question. Extracts of animals, plants, or yeasts Cell secretions Sodium chloride Disodium hydrogen phosphate Blood and serum
Blood and serum Extracts of animals, plants, or yeasts Cell secretions
Media ingredients that cannot be chemically defined include which of the following? Multiple select question. Blood and serum Sodium chloride Disodium hydrogen phosphate Cell secretions Extracts of animals, plants, or yeasts
Cell secretions Extracts of animals, plants, or yeasts Blood and serum
When adequately separated from other cells, an individual bacterial cell can grow into a discrete mound of cells called a(n) _____________
Colony
All of the following are properties used to classify media, except ______. chemical composition color functional type physical state
Color
Microbial growth in a liquid broth medium is indicated by ______. Multiple select question. volume gain volume loss color change cloudiness sediment
Color Change Cloudiness Sediment
When a culture shows two or more microbes of uncertain identity that have been introduced into it, the culture is considered to be ______. Multiple choice question. Mixed contaminated axenic/pure
Contaminated
Which term describes a once pure culture that has since had unwanted microbes of uncertain identity introduced into it? Contaminated Mixed Axenic Differential
Contaminated
Which of the following media can be described precisely as to its chemical content? Defined General purpose Complex Brain-Heart Infusion Broth
Defined
Which of the following media can be described precisely as to its chemical content? General purpose Defined Complex Brain-Heart Infusion Broth
Defined
True or false: "Inspection" begins the process using the Five "I" procedure for culturing microorganisms.
False
True or false: A defined medium differs from a complex medium in that the chemical composition of the defined medium cannot be precisely determined.
False
True or false: Selective media cannot be differential.
False
A temperature-controlled chamber that provides the proper temperature conditions for microbial multiplication in the culturing process is called a(n) __________________________.
Incubation
In which of the five I's would a microbiologist adjust the temperature and atmospheric gases in a culture environment? Inspection Incubation Identification Isolation Inoculation
Incubation
Which of the following describes the process of placing a culture of microorganisms in a temperature-controlled chamber to encourage growth? Inspection Identification Inoculation Incubation
Incubation
Which of the following identifies the step in the Five I's of culturing microorganisms during which a culture is placed in a controlled environment to encourage growth? Identification Inoculation Incubation Inspection
Incubation
Which one of the Five I's involves placing an inoculated container of medium in a termperature-controlled chamber to encourage multiplication of the microbes? isolation inoculation incubation identification inspection
Incubation
Which one of the following is NOT part of the 5 basic techniques used to grow, examine and manipulate microorganisms in the laboratory (recall the Five I's)? Inspection Infection Isolation Inoculation
Infection
Which one of the following is NOT part of the 5 basic techniques used to grow, examine and manipulate microorganisms in the laboratory (recall the Five I's)? Inspection Inoculation Infection Isolation
Infection
The introduction of microorganisms into an environment in which they will multiply is called
Inoculation
Which one of the five basic techniques refers to the introduction of a tiny sample into a container of nutrient medium so that microorganisms can multiply? Isolation Inspection Inoculation Incubation Identification
Inoculation
_________________ is part of the Five I's of culturing microorganisms and is based on visual observations of microscopic and macroscopic growth characteristics.
Inspection
DNA detection technologies have shown that there are ______ microbes that we don't know how to cultivate in the lab than those we do. about as many many more many fewer
Many more
A container that holds two or more identified, easily differentiated species of microbes is called a _____________ culture.
Mixed
If a culture contains two or more identified, easily differentiated microbe species, it is called a ______ culture. mixed contaminated pure differential
Mixed
Liquid is one of the ______ states which is used to classify microbial media. nutrient chemical biological physical
Physical
A property used to classify media that refers to the medium's normal consistency is the __________ __________.
Physical state
When a container of medium grows only a single known species or type of microorganism, that culture is considered ______. single clean aseptic pure
Pure
Sabouraud's agar restricts the growth of bacteria and is an example of a ______________ medium, whereas Mannitol salt agar can determine which species of Staphylococcus is present, making it an example of a ______________ medium.
Selective Differential
Microbial media can be classified as which of the following physical states? Multiple select question. complex solid acidic semisolid liquid
Solid, Liquid, Semisolid
Which of the following statements regarding culturing microbes is true? All microbial diseases are caused by microbes that can be cultured in hospital laboratories. It is rare to find a microbe that cannot be grown on an enriched artificial medium. Some microbes found in nature can be grown on artificial medium but most cannot.
Some microbes found in nature can be grown on artificial medium but most cannot.
Which of the following factors that affect the growth of microbes can be controlled by incubators? Multiple select question. temperature sterility atmospheric gas medium composition
Temperature Atmospheric gas
The techniques used to prepare specimens for optical microscopy depend on ______. The condition of the specimen The aims of the examiner The number of microbes present The type of microscopy available
The condition of the specimen The aims of the examiner The type of microscopy available
True or false: The aims of the examiner, such as whether to observe overall structure or to observe motility, influence the manner in which specimen is prepared for optical microscopy.
True
Staining involves ______. observing cells under a light microscope fixing cells to a glass slide for observation applying colored dyes to specimens applying a liquid suspension of cells to a glass slide
applying colored dyes to specimens
Especially with bacteria and fungi, the most common manifestation of growth on solid media is the appearance of __________________.
colonies
Mound of cells
colony
A culture that results when a microbe of uncertain identity has been introduced into it is called a(n) ______________ culture.
contaminated
When a culture shows two or more microbes of uncertain identity that have been introduced into it, the culture is considered to be ______. contaminated axenic/pure mixed
contaminated
The visualization of bacteria requires the use of a compound _________________ microscope.
light
A specimen for optical microscopy is generally prepared first by ______. mounting the sample on a glass slide killing the sample staining the sample in solution using a fixative on the sample
mounting the sample on a glass slide
For a light microscope, the lens that is normally located directly above the stage is called the _________________ lens.
objective
An image's total magnification is determined by multiplying the individual power of both the ______. objective lens and the condenser objective lens and the aperture objective lens and the ocular lens ocular lens and the iris diaphragm
objective lens and the ocular lens
The total power of magnification is calculated by the product of the separate powers of the ______________ lens and the _________________ lens.
ocular objective
A specimen for ________________ microscopy is generally prepared first by placing a sample on a glass slide.
optical
A culture that contains only ONE species of microorganism is called a(n) ___________ culture.
pure
Any procedure that applies colored chemicals to specimens is called ______. shadowing staining marking fixing
staining