Microbiology 440 (chapter 2)

A polysaccharide that acts as a solidifying agent in microbiological media is called ______________.

Agar

The polysaccharide found in a sea alga that is commonly used to prepare solid culture media is called ______. Peptone Starch Gelatin Agar

Agar

Which type(s) of organisms are usually observed with light microscopes? Viruses Prions Bacteria Arthropods

Bacteria

Media ingredients that cannot be chemically defined include which of the following? Multiple select question. Extracts of animals, plants, or yeasts Cell secretions Sodium chloride Disodium hydrogen phosphate Blood and serum

Blood and serum Extracts of animals, plants, or yeasts Cell secretions

Media ingredients that cannot be chemically defined include which of the following? Multiple select question. Blood and serum Sodium chloride Disodium hydrogen phosphate Cell secretions Extracts of animals, plants, or yeasts

Cell secretions Extracts of animals, plants, or yeasts Blood and serum

When adequately separated from other cells, an individual bacterial cell can grow into a discrete mound of cells called a(n) _____________

Colony

All of the following are properties used to classify media, except ______. chemical composition color functional type physical state

Color

Microbial growth in a liquid broth medium is indicated by ______. Multiple select question. volume gain volume loss color change cloudiness sediment

Color Change Cloudiness Sediment

When a culture shows two or more microbes of uncertain identity that have been introduced into it, the culture is considered to be ______. Multiple choice question. Mixed contaminated axenic/pure

Contaminated

Which term describes a once pure culture that has since had unwanted microbes of uncertain identity introduced into it? Contaminated Mixed Axenic Differential

Contaminated

Which of the following media can be described precisely as to its chemical content? Defined General purpose Complex Brain-Heart Infusion Broth

Defined

Which of the following media can be described precisely as to its chemical content? General purpose Defined Complex Brain-Heart Infusion Broth

Defined

True or false: "Inspection" begins the process using the Five "I" procedure for culturing microorganisms.

False

True or false: A defined medium differs from a complex medium in that the chemical composition of the defined medium cannot be precisely determined.

False

True or false: Selective media cannot be differential.

False

A temperature-controlled chamber that provides the proper temperature conditions for microbial multiplication in the culturing process is called a(n) __________________________.

Incubation

In which of the five I's would a microbiologist adjust the temperature and atmospheric gases in a culture environment? Inspection Incubation Identification Isolation Inoculation

Incubation

Which of the following describes the process of placing a culture of microorganisms in a temperature-controlled chamber to encourage growth? Inspection Identification Inoculation Incubation

Incubation

Which of the following identifies the step in the Five I's of culturing microorganisms during which a culture is placed in a controlled environment to encourage growth? Identification Inoculation Incubation Inspection

Incubation

Which one of the Five I's involves placing an inoculated container of medium in a termperature-controlled chamber to encourage multiplication of the microbes? isolation inoculation incubation identification inspection

Incubation

Which one of the following is NOT part of the 5 basic techniques used to grow, examine and manipulate microorganisms in the laboratory (recall the Five I's)? Inspection Infection Isolation Inoculation

Infection

Which one of the following is NOT part of the 5 basic techniques used to grow, examine and manipulate microorganisms in the laboratory (recall the Five I's)? Inspection Inoculation Infection Isolation

Infection

The introduction of microorganisms into an environment in which they will multiply is called

Inoculation

Which one of the five basic techniques refers to the introduction of a tiny sample into a container of nutrient medium so that microorganisms can multiply? Isolation Inspection Inoculation Incubation Identification

Inoculation

_________________ is part of the Five I's of culturing microorganisms and is based on visual observations of microscopic and macroscopic growth characteristics.

Inspection

DNA detection technologies have shown that there are ______ microbes that we don't know how to cultivate in the lab than those we do. about as many many more many fewer

Many more

A container that holds two or more identified, easily differentiated species of microbes is called a _____________ culture.

Mixed

If a culture contains two or more identified, easily differentiated microbe species, it is called a ______ culture. mixed contaminated pure differential

Mixed

Liquid is one of the ______ states which is used to classify microbial media. nutrient chemical biological physical

Physical

A property used to classify media that refers to the medium's normal consistency is the __________ __________.

Physical state

When a container of medium grows only a single known species or type of microorganism, that culture is considered ______. single clean aseptic pure

Pure

Sabouraud's agar restricts the growth of bacteria and is an example of a ______________ medium, whereas Mannitol salt agar can determine which species of Staphylococcus is present, making it an example of a ______________ medium.

Selective Differential

Microbial media can be classified as which of the following physical states? Multiple select question. complex solid acidic semisolid liquid

Solid, Liquid, Semisolid

Which of the following statements regarding culturing microbes is true? All microbial diseases are caused by microbes that can be cultured in hospital laboratories. It is rare to find a microbe that cannot be grown on an enriched artificial medium. Some microbes found in nature can be grown on artificial medium but most cannot.

Some microbes found in nature can be grown on artificial medium but most cannot.

Which of the following factors that affect the growth of microbes can be controlled by incubators? Multiple select question. temperature sterility atmospheric gas medium composition

Temperature Atmospheric gas

The techniques used to prepare specimens for optical microscopy depend on ______. The condition of the specimen The aims of the examiner The number of microbes present The type of microscopy available

The condition of the specimen The aims of the examiner The type of microscopy available

True or false: The aims of the examiner, such as whether to observe overall structure or to observe motility, influence the manner in which specimen is prepared for optical microscopy.

True

Staining involves ______. observing cells under a light microscope fixing cells to a glass slide for observation applying colored dyes to specimens applying a liquid suspension of cells to a glass slide

applying colored dyes to specimens

Especially with bacteria and fungi, the most common manifestation of growth on solid media is the appearance of __________________.

colonies

Mound of cells

colony

A culture that results when a microbe of uncertain identity has been introduced into it is called a(n) ______________ culture.

contaminated

When a culture shows two or more microbes of uncertain identity that have been introduced into it, the culture is considered to be ______. contaminated axenic/pure mixed

contaminated

The visualization of bacteria requires the use of a compound _________________ microscope.

light

A specimen for optical microscopy is generally prepared first by ______. mounting the sample on a glass slide killing the sample staining the sample in solution using a fixative on the sample

mounting the sample on a glass slide

For a light microscope, the lens that is normally located directly above the stage is called the _________________ lens.

objective

An image's total magnification is determined by multiplying the individual power of both the ______. objective lens and the condenser objective lens and the aperture objective lens and the ocular lens ocular lens and the iris diaphragm

objective lens and the ocular lens

The total power of magnification is calculated by the product of the separate powers of the ______________ lens and the _________________ lens.

ocular objective

A specimen for ________________ microscopy is generally prepared first by placing a sample on a glass slide.

optical

A culture that contains only ONE species of microorganism is called a(n) ___________ culture.

pure

Any procedure that applies colored chemicals to specimens is called ______. shadowing staining marking fixing

staining