microbiology 8 & 10 lab quiz 2

ignatz semmelweis

discovery of the importance of hand and skin surface antisepsis is credited to _________ _________

isolated a pure culture/single organism

do not run a sensitivity test until you have ______________

exposure time, concentration required to kill microbes, temperature and ph for its optimal activity, concentration of the microbe present and the toxicity of the agent for tissue or its effect on materials being disinfected

factors to consider when choosing a disinfectant

intermediated sensitivity

grey area

when they are dividing

in which growth phase are bacteria most sensitive to antibiotics

cidal

kills organism

not going to be source of contamination, will not be weakened by organic material

list 2 advantages chlorhex has over povidone iodine (betadine) for disinfection of hands

normal flora

microorganisms that are permanent are called _______________ and are very important to the future health of the offspring

static

stops growth of organism

zone of inhibition

to examine the MH antibiotic sensitivity plate we measure the size of each _____________________

the longer the scrubbing the less bacterial growth

what effect did the duration of chlorhexidine scrubbing have on bacterial growth?

less bacterial growth

what effect did the paper towel have on the water only procedure?

less bacterial growth

what effect did the waiting have on the waterless cleaner procedure?

ethyl alcohol

what is the active ingredient in the waterless cleaner?

enfrofloxacin, azithromycin, trimethoprim-sulfa, gentamicin, ciprofloxacin, amoxicillin/clavulanate

which antibiotics were effective against the specific organism tested and could be a good treatment choice?

rescue cleaner; hydrogen peroxide; bacteriocidal

which disinfectant was the most effective against the organism tested? what is the active ingredient? bacertiocidal/bacteriostatic?

Mueller-Hilton (clear for accurate results)

which media is used for agar diffusion disk method?

roccal, betadine

which of the disinfectants tested is/are a poor choice in the presence of organic debris?

chlorhexidine

which washing technique was most effective at removing bacteria?

debris can get in our solutions

why do we care about the connection between organic debris and disinfectants?

no, it increases osmotic pressure thus bursting the bacterial cells

would you predict "cillin" antibiotics to be effective against gram negative organisms? how do the cilia antibiotics kill bacteria?

agar diffusion disc method (kirby bauer test); tube dilution technique (mic determination)

2 common testing methods for C/S testing

culture and sensitivity

C/S

joseph lister

_________ _________ in the 1860's used semmelweis's information to establish his methods for surgical asepsis

resistant

bacteria that are ___________ to Drug A will grow in the presence of drug A

susceptible/sensitive

bacteria that are __________________ to Drug A will not grow in the presence of drug A