Microbiology Automation
instrument QC includes (1) _____ maintenance program (2) daily _____ checks (3) weekly _____ QC (4) new _____
(1) preventive (2) temperatures (3) organism (4) panels/cards
in automated rapid systems for microbiol panels are (3 things) by the system software
(1) incubated (2) read (3) interpreted
even with recent automated tech, MLS is still needed to (1) perform ____ (2) interpret ____ (3) analyzed _____ of specimen and info
(1) initial readings (2) lab results (3) quality of specimen and info
In ARSMI, color change can be detected (1) ____ by user; reading code book for biotype and ID or (2) detected _____ by system instrument spectroscopy; ID by collating info and matching to database
(1) manually (2) automatically
name the 2 steps in order after a bottle is flagged as pos in the verstrek diagnostic system
(1) 1st step = gram stain (2) notify
2 clinical microbiol tasks and objectives (1) isolation and identification of ____ (2) determine susceptibility studies on ____
(1) agents of infection from patient specimens (2) those isolated and identified agents
plate inoculators on the market (1) [are/are not] extensively used because (2) ____
(1) are not (2) still need development
4 areas that uses current instrumentation and automation in microbiol
(1) blood culture systesm (2) bacterial identification and susceptibility systems (3) immunoassay systems (4) molecular methods
in automated rapid systems for microbial identification generally (1) the computer software _____ (2) interfaces with _____ (3) has a turnaround time of usually ____
(1) collates the info, matches to a database and report isolate identifications in %s (2) susceptibility cards (3) <8hrs
3 advantages of instrumentation and automation in blood culture systems (1) early ____ (2) detection of ____ (3) decreased ____
(1) early detection of bacterial growth (2) detection of slow growing bacteria s/a mycobacteria (3) decreased tech time
2 advantages of current instrumentation and automation in microbiol for molecular systems
(1) helpful when organisms cannot be successfully cultured (2) rapid info for doctor
3 disadvantages of instrumentation and automation in blood culture systems (1) high initial ____ (2) cannot detect some ____ (3) possible _____
(1) high initial cost of instrument (2) cannot detect some fastidious organisms (3) possible contamination
molecular technology in microbial include (1) DNA probe testing to ____ and (2) _____ labels
(1) identify microorganisms (2) chemiluminescent
prior to 1980, the traditional microbiol standard were 3 things (1) agar plate and enrichment broths for _____ (2) test tubes for ____ (3) Kirby-bauer plates and broth dilute tubes for _____
(1) primary isolation (2) identify isolates (3) susceptibility studies of isolates
routine laboratory methods for detecting MRSA do not support a (1) _____ but (2) _____ solves that problem
(1) rapid detection system (2) molecular technology
SCCmec-orfX stands for (1) _____ and is used for (2) _____
(1) staphylococcal cassette chromosome open reading frame X used (2) real time PCR
automated rapid systems for microbiol identification (1) are reported as ____ (2) has rapid ____ (3) interface with ____
(1) statistical prediction of correct identification thats based on a database (2) turnaround time usually <24hrs (3) laboratory or hospital information systems
most automated rapid systems for microbial identification use 3 types of assay principles
(1) turbidity (2) colorimetry (3) fluorescent
one of the most widely used assays in non reference microbiol labs for MRSA and VRE
PCR in microbiol used for methicilin-resistant Staph aureus and vancomycin-resistant enterococci
activated charcoal media
Pedi
name this system (1) noninvasive, continuous monitoring using barcodes (2) pH sensitive membrane in bottom of bottles (3) pH change resulting from CO2 production indicated by color change (4) instrument measures CO2 production colorimetrically (5) test each bottle 144x/day and plots growth curve from plot of reflectance vs time
bact-alert or biomerieux blood culture systems
system that has a bottle that contains internal colorimetric sensor on the bottom that detects CO2 as a measure of bacteria growth.
bact-alert or biomerieux blood culture systems
BacT-Alert or bioMerieux is a ____ system
blood culture
in the bact-alert or biomerieux blood culture systems, if bacteria are present in patients blood, they will produce CO2 and the sensor on the bottom of the bottle will change from ____ to ____
blue green to yellow
name the assay (1) real time PCR assay for the detection of the SCCmec-orfX (2) currently the most rapid of all the commerical methods
cepheid xpert MRSA assay
in nucleic acid hybridization, binding between target and probe occurs based on _____
complementary base sequences
in nucleic acid hybridization, the other strand (usually the probe) is _____ to the target and is labeled
complementary in sequence
MRSA is located in the nares, skin, and on mucosal surfaces of the vagina and rectum. Carriers have a high likelihood of _____
developing and transmitting the infections
automated rapid systems for microbial identification use panels of ____ or ___ reagents provided in microtiter trays or sealed cards
freeze-dried or lyophilized
VRE is usually carried in the _____
intestine
name the system (1) substrates conjugated with fluorophores and substrates with a fluorescent pH indicator (2) wells inoculated with suspension of organism (3) 2hrs incubattion
microscan system (automated rapid systems for microbial identification)
systems used automatic identification, barcode verification and machine vision applications
microscan walkaway (automated rapid systems for microbial identifcation)
name the system (1) robot arms move trays to reader at the correct time (2) oil overlay and reagent are added to the proper wells (3) read, interpret and print panel results (4) does require CLS interpretations (5) rapid fluorescence panels and conventional panels available
microscan walkway
____ 7H9 broth is a liquid growth medium specially used for culture of mycobacterium spp
middlebrook
in nucleic acid hybridization, one strand (usually the target) is a unique _____
nucleic acid sequence from the organism to be identified
Nuclein acid hybridization assays require detection of two ___
nucleic acid strands
name the technique uses (1) dsDNA denatured (2) primers and beacons attach to the target (3) becon's confirmation changes and light is emitted because dyes move apart (4) DNA polymerase starts to build a sequence that is complimentary to target until it knocks the beacon off
real time PCR using molecular beacon to detect target
designed for rapid qualitative isolation and differentiation of pathogens from blood s/a mycobacteria
septicheck blood culture system
general purpose liquid enrichment medium used in qualitative procedures for the sterility test and for the enrichment and cultivation of aerobic microorganisms that are not too fastidious
tryptic soy broth or TSB
yellow IRIS is an automated ____ screen instrument
urine
name this system (1) continuous monitoring (aerobic every 12mins, anaerobic every 24mins) (2) gases detected in headspace pressure (3) internal computer monitors changes and determines when to flag a bottle as pos
verstrek from trek diagnostic systems
(1) a "space-age product" created in 1960 by mcdonnel douglas (2) 64 microwell cards contain substrates for identification of bacteria and AST (3) suspension and card placed into a rack (cassete), card is filled and placed into an incubator-reader module (4) optically scanned and read every 15mins
vitek2 system (automated rapid systems for microbial identifcation)