Module 5: Creating, Staining and Evaluating Peripheral Blood Films

Identify the three areas of a blood film

body, monolayer, feathered edge

When evaluating a blood film you use the 100x oil immersion objective. If your microscope has 10x ocular lenses what would the total magnification of the cells on the blood film be?

1,000 times

The monolayer of a peripheral blood film contains red blood cells that are:

side by side, sometimes touching

The section of the blood film labeled A is known as the:

Feathered Edge

When making a blood film on an animal with a low hematocrit you may need to:

Increase the angle of the spreader slide

You made a blood film and stained it using a rapid type stain (Ex: Dif Quik). Upon microscopic evaluation you note that the red blood cells are purple and the white blood cells are bluer in appearance than normal. What changes in staining technique would be needed to correct this problem?

Use shorter dips in solution 2; Use longer dips in solution 1