Yeast complexes
RAD26-DEF1 stalled RNAPII response complex
Appears to form in response to DNA damage-stalled RNA polymerase II, when the stall is persistent such as at DNA lesions
HICS complex
Appears to have a role in cytokinesis. May connect the actin-myosin ring to the plasma membrane
BUG1-GRH1 complex
Appears to play a role in the tethering of COPII, though an interaction with Sec23/24, and thus formation of the cis-Golgi and ER to golgi vesicle-mediated transport. The complex is anchored to membranes thorugh an acetylated amino-terminal region in Grh1
TRM9-TRM112 methyltransferase complex
S-adenosylmethionine-dependent methyltransferase involved in the formation of mcm5(s2)U(5-methoxycarbonylmethyl(2-thio)uridine) modifications at position 34 from the anticodon loop of some tRNAs
Gamma tubulin small complex
Seeds microtubule nucleation at microtubule-organizing centers, controlling the location and timing of nucleation. Component of the spindle pole body
DCS1-DCS2 regulator of decapping scavenger complex
m7G(5-prime)pppN diphosphatase with a kcat/KM value that is significantly lower than that of the Decapping scavenger complex and has almost lost the preference for m7GpppG as a substrate shown by the homodimer
Global genome repair CUL3/RAD7/RAD16/ELC1 ubiquitin ligase complex
A ubiquitin ligase complex required for optimal nucleotide excision repair following UV-induced DNA damage
Nuclear MIS12/MIND complex
A kinetochore complex that assembles independently onto centromeric DNA, required for the spindle checkpoint and kinetochore integrity. MIND plays a role in establishing a bipolar spindle-kinetochore interaction by joining kinetochore subunits contacting DNA to those contacting microtubules
DNF2-LEM3 P4-ATPase complex
A membrane pump which actively translocates, or flips, phospholipids across cell membranes from the exoplasmic to the cytoplasmic leaflet of the lipid bilayer. This generates and maintains membrane lipid asymmetry, a property essential for a wide variety of cellular processes such as vesicle budding and intracellular vesicle trafficking.
DNF3-CRF1 P4-ATPase complex
A membrane pump which actively translocates, or flips, phospholipids across cell membranes from the exoplasmic to the cytoplasmic leaflet of the lipid bilayer. This generates and maintains membrane lipid asymmetry, a property essential for a wide variety of cellular processes such as vesicle budding and intracellular vesicle trafficking.
DRS2-CDC50 P4 ATPase complex
A membrane pump which actively translocates, or flips, phospholipids across cell membranes from the exoplasmic to the cytoplasmic leaflet of the lipid bilayer. This generates and maintains membrane lipid asymmetry, a property essential for a wide variety of cellular processes such as vesicle budding and intracellular vesicle trafficking.
cytoplasmic dynein complex
A microtubule minus end-directed motor complex involved in positioning the mitotic spindle during cell division.
Central kinetochore CTF19 complex
Present in the central kinetochore, where it appears to play a role in the enhancement of cohesin in the pericentromere. May help to mediate the attachment of the centromere to the mitotic spindle by forming interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins
Importin complex, KAP60-KAP95
A nuclear import complex that functions as a nuclear import receptor for proteins containing a nuclear localisation signal (NLS). Subunit SRP1 specifically and directly binds to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is me
PIP2-OAF1 transcription factor complex
Acts as a transcriptional activator to induce the transcription of genes encoding proteins involved in fatty acid beta-oxidation, a response called oleic acid induction, when cells grow on fatty acids as the sole carbon source. Recognizes and binds to the oleate response element
GAL4-GAL80 transcription repressor complex
Acts to repress the GAL4 positive regulator of gene expression. The GAL network is a small set of genes that regulates galactose import and metabolism. The transcriptional activator GAL4 activates a set of enzymatic and regulatory genes by binding to their promoter regions.
SWI/SNF chromatin remodeling complex
An ATP-dependent chromatin remodelling complex which disrupts the nucleosome structure, increases the binding of transcription factors to nucleosomes, mobilizes histone octamers along DNA in cis, transfers histone octamers to different DNA fragments, displaces histone H2A/H2B dimers and generates superhelical torsion in DNA
DPB11-SLD3-SLD2 DNA replication complex
Associate with replication origins and promotes loading of DNA polymerases onto the origins to initiate chromosomal DNA replication when cyclin-dependent kinase activity increases at the G1/S cell cycle boundary
Fatty-acyl-CoA synthase
Catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. Consists of a multifunctional enzyme composed for two non-identical alpha and beta subunits which are organized in an alpha6beta6 complex with 6 sites of fatty acid synthesis
Ribonucleoside-diphosphate reductase variant 1
Catalyzes the reduction of ribonucleotides to the corresponding deoxyribonucleotides, an essential step in the de novo synthesis of monomeric precursors for DNA replication and repair.
SKI complex
Central component of the 3'-5' cytoplasmic mRNA degradation pathway which interacts with Ski7 to mediate degradation by the exosome. The Ski complex appears to thread RNAs directly to the exoxome, coupling the helicase and the exoribonuclease through a continuous RNA channel
TUL1 E3 ubiquitin ligase complex
E3 ubiquitin ligase complex required in cells lacking the multivesicular body pathway and under condition of ubiquitin depletion. Functions in protein homeostasis under non-stress conditions and support a role in protein quality control
cleavage and polyadenylation specificity factor complex
Endonuclease complex required for mRNA 3' end processing to form a defined 3' end of the transcribed RNA. The complex cleaves pre-mRNAs, adds a polyadenylate tail, and triggers transcription termination.
BLOC-1 complex
Endosomal Rab-GAP adapter complex which controls the lifetime of active Rab5/Vps21 and thus endosomal maturation along the endocytic pathway
NEO1-MON2-ARL1-DOP1 membrane remodeling complex
Functions in vesicle trafficking within the golgi/ensosomal system with a putative role in membrane remodeling at the tubular endosomal network and the trans-golgi network interface
Transcription factor TFIIF complex
General transcription factor that interacts with RNAPII and can recruit RNAPII to a preinitation complex containing TFIID and TFIIB. Required for the opening of DNA, to allow transcription initiation. DNA opening occurs around the tip of the Pol II clamp and the TFIIE
DASH complex
Heterodecameric component of the kinetochore necessary for accurate chromosome segregation, supporting the dynamic attachment of mitotic chromosomes to the ends of shortening spindle microtubules
COMPASS complex
Histone methyltransferase that catalyzes methylation of Lys-4 of histone H3 and thus controls the silencing of telomeric regions. SWD1, SWD3, and SET1 constitute a core of the complex, and the absence of any of these subunits abolishes methylation of lysine 4 on histone H3.
DNA polymerase zeta complex
Low-fidelity non-essential DNA polymerase that is involved in translesion DNA synthesis. Required for the majority of spontaneous mutagenesis in wild-type yeast cells, as well as for mutagenesis associated with transcription, with DSBR and with defective DNA repair
mRNA nuclear export factor complex, MEX67, MTR2
Mediates the export of bulk mRNA through direct interactions with both mRNA cargo and nuclear pore proteins that contain characteristic phenylalanine-glycine repeating sequence motifs. Also contributes to ribosomal subunit export
RecQ helicase-Topo III complex
Required for both the early and late steps of DNA double-strand break (DSB) via dissolution of double Holliday junctions. Early in the repair of a two-ended DSB, the complex contributes to DSB end resection by facilitating the formation of a single-strand 3-prime overhang on which the homologous recombination factor Rad51 filament assembles
N-acetylglutamate synthase NAGS/NAGK complex
Required for the controlling initial step of L-arginine biosynthesis. Forms a metabolon, ie a complex formed by the supramolecular association of two sequentially acting enzymes of the pathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate
CoQ biosynthetic complex
Required for the synthesis of Coenzyme Q, and isoprenylated benzoquinone which functions as an electron carrier from complex I or II to complex III in the inner mitochondrial membrane and which also acts as an antioxidant preventing the oxidation of lipoproteins and the plasma membrane
sulfur metabolism transcription factor complex
Transcription factor complex regulating sulfur metabolism. MET4 lacks DNA-binding ability and relies on interactions with MET31 and MET32, paralogous proteins that bind the same cis-regulatory element, to activate its targets.
RAP1-GCR1 transcription activation complex
Transcription factor required for the transcription of ribosomal protein and glycolytic genes. Appears to simultaneously bind to two adjacent DNA elements
ARGR-MCM1 transcription regulation complex
Transcriptional regulation complex which binds, in the presence of arginine, to the promoter regions of genes co-regulated by arginine, repressing the synthesis of five anabolic enzymes, e.g. ornithine carbmoyltransferase and inducing the synthesis of two catabolic enzymes
MOT1-TBP transcription regulation complex
Transcriptional regulation complex, formation of which causes the dissociation of the TATA-binding protein from promoters and thereby regulates TBP distribution in the cell
Vacuolar proton translocating ATPase complex, Golgi variant
Translocates protons across a lipid bilayer via an ATP-driven rotary mechanism, thus acidifying the lumen of its resident organelle. As newly synthesized proteins transverse the golgi apparatus they undergo post-translational modifications, including glycosylation, sulfation, and phosphorylation, and are targeted to their appropriate destinatino in a pH-dependent manner.
ERV41-ERV46 retrograde receptor complex
Transporter complex that recognises binds, and returns endoplasmic reticulum resident proteins that have trafficked to golgi compartments. Targets proteins lacking the HDEL motif recognized by COPI-coated vesicles. Transported back to Golgi by COPII for the next round of retrograde transport
CUL8-MMS1-MMS22-CTF4 E3 ubiquitin ligase complex
Ubiquitin ligase complex required for the ubiquitination of acetylated histone H3, thus facilitating nucleosome assembly during replication and promoting replication progression during S-phase
DNA mismatch repair MutSalpha complex
mismatch repair complex, involved in the recognition and repair of base-base and small insertion/deletion mismatches that appear as a conseqeunce of DNA polymerase errors during DNA synthesis. The complex is most effective at repairing mismatches on the lagging strand of replication
FAR complex
required for pheromone-induced cell cycle arrest and for caspase-10-induced death
