2,3 DPG, how is it produced in RBC and how does it interact with Hb; what is its relevance in altitude exposure, anemia and stored blood

what is the interactino of 2,3 DPG with Hb?

1:1 binding of beta subunit of deoxy Hb molecules conformational change in Hb molecule leading to right shift of ODC curve (Bohr effect) this increase off loading at low pO2 shifts p50 to the right reduce affinity of O2

how is 2,3 DPG produced? What does the pathyway called?

2,3 DPG produced in RBC as side chain of glycolysis by Embden-Meyerhof pathyway ( RBC has a mayer, called Emden mayer off) Found in cell not dependent on aerobic metabolism

what is 2,3 DPG

2,3 diphosphoglycerol

what is Bohr effect ?

Physiological phenomenon first described in 1904 by the Danish physiologist Christian Bohr, stating that hemoglobin's oxygen binding affinity is inversely related both to acidity and to the concentration of carbon dioxide

why in fetal OHDC is not affected by the production of 2,3 DPG

because fetal Hb is 2alpha 2 gamma; 2,3 DPG only binds to beta subunit of Hb

Production of 2,3 DPG is increased by what?

increase in temp, pH of plasma reduce in pO2, as in COPD, altitude and anemia

Anemia effect on 2,3 DPG

it's relative hypoxia, increase 2,3 DPG right shift of ODC, reduce O2 affinity, enhance unloading, p50 is 4 mmHg higher than normal

altitude effect on 2,3 DPG

reduce PaO2, increase RR, MV, increase pH (increase 2,3 DPG ) causing right shift of ODC, p50 increase, enhance unloading of oxygen increase oxygen carrying capacity to peripheries in light of low pO2 at the expense of reduce loading of O2 pulmonary capillary

stored blood effect on 2,3 DPG

stored blood, temperature reduced reduce glycolysis in RBC, reduce 2,3 DPG Left shift of ODC the production restored in 24 hrs of transfusion