A&P 2 Lab: Ex 4 White blood cells

Be prepared to identify projected WBCs (in color: use digital images provided on our course website).

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Describe the general features of: Monocytes.

Are largest circulating leukocytes, 2-3X the size of surrounding RBC's. they are some clear, light blue cytoplasm surrounding a nucleus that's bi-lobed. they transform into macrophages which then enter other body tissues.

Wright's Stain uses a combination of red acidic stain called __________ and an alkaline (basic) stain called ___________.

Eosin Methylene Blue

How is a Differential White Cell Count performed?

Examine your slide using the high dry objective.. then apply oil and carefully rotate the oil objective lens into place, adjust with fine adjustment knob and iris diaphragm. Perform tally of WBC'S by marking off each one that you see by type. Each tally will represent one percentage point.

Describe the general features of: Eosinophils

Have an affinity for the Eosin (alkaline) in Wright stain. Granules are in pink-red range.

Describe the general features of: Basophils

Have an affinity for the Methylene blue (which is "basic"). Their large, chunky granules are blue-purple in color. Granules are dark and large that sometimes it is difficult to see the obscured nucleus of a basophil.

Describe the Wrights Stain procedure.

Lay down paper towels, then add 1-3 drops of Wright Stain to sample of the slide until the blood is completely covered. Let stain sit for 2-3 Minutes, Then add same number of drops of the buffer. Blow on the side to mix the stain and buffer. Let the slide sit until you see metallic greenish film or until 8 mind (do not go past 8 mins). Hold slide down with clothespin over sink and rinse with water. Place slide between bibulous paper and remove clothespin.

Which leukocytes are roughly the same size as the surrounding RBCs?

Lymphocytes

What are agranulocytes?

Lymphocytes, Monocytes

Which leukocyte is the largest?

Monocytes

If a cell has more than 3 lobes to its nucleus it's probably a __________.

Neutrophil

what are granulocytes?

Neutrophils, Eosinophils, Basophils.

Never Let Monkeys Eat Bananas

Neutrophils, Lymphocytes,Monocytes,Eosinophils,Basophils

Describe the general features of:Lymphocytes

Smallest of WBC's. nuclei are speherical and seem to fill. a thin rim of cytoplasm at the periphery of each cell.

True or false: neutrophils are called polymorphonuclear cells because they contain many differently-shaped nuclei.

True

How is a blood smear prepared?

Wash slides and wash both hands, clean finger (pinky finger) with alcohol pad. Obtain a drop of blood and immediately apply it towards the end on one side. Without delay use second slide a spreader, pulling blood across to he end of the slide in one smooth motion. Lay down on paper towel and completely dry. If extra blood prop slide up at an angle by placing one end over the spreader slide.

Cells with pink-staining granules are __________, while cells with dark blue-staining granules are ___________.

eosinophils basophils

Describe the general features of: Neutrophils

have nuclei with several lobes (3-6), does not have several nuclei per cell but that are just lobes of a single nucleus projecting upward. Cells have equal attraction to the two components of Wright Stain. Granules are a pale lavender color.