ascp

*Distinguishing Staphylococci from micrococci *Presumptive identification of Group A streptococci

Bacitracin susceptibility is useful for which two of the following:

Platelets

Bacterial contamination is MOST likely in which of the following blood products?

Cell mediated, humoral, innate, and non-specific are all branches of the immune system. Extrinsic is a branch of the coagulation cascade.

Branches of the immune system

1:20

Dilution for routine sperm count

Calcium

EDTA and Sodium citrate combine with _________ to inhibit coagulation in blood samples.

Multiple myeloma *Dehydration Lupus erythematosus *Orthostatic proteinuria

Examples of conditions resulting in benign proteinuria include: (Choose ALL correct answers)

biliary obstruction

Fecal urobilinogen level may be markedly decreased because of:

Prevent Graft-Versus-Host (GVH) disease

Gamma irradiation of cellular blood components is required in which of the following situations:

anti-A1

Lectin from the seeds of Dolichos biflorus is a source of which of the following?

(IgG-IgM) Fixes complement via classical pathway (IgM) Largest antibody, pentamer (IgG) Crosses placental barrier (IgE) Immediate Hypersensitivity

Match antibody group with corresponding descriptor:

PSA, or prostate specific antigen = Prostate CEA, or Carcinoembryonic Antigen = Colon beta hCG, or Human chorionic gonadotropin = Testicles AFP, or Alpha-fetoprotein = Liver

Match the tumor markers below with their corresponding sites of tumor origin.

Arbovirus: Encephalitis Hepadnavirus: Hepatitis Paramyxovirus: Measles-Mumps Parvovirus: Fifth disease

Match the viruses below with their associated diseases.

low PH

Metabolic acidosis is characterized by:

37 Degrees Celsius

Sperm should be kept at, prior to collection

lipids

Substances found in urinary sediment that are more easily distinguished by use of polarized microscope are:

Kell

The McLeod phenotype is associated with which of the following antigen systems?

kell

The McLeod phenotype is associated with which of the following antigen systems?

Streptococci pneumoniae

The Optochin (ethylhydrocupreine hydrochloride) disc is MOST often used for the identification of which organism?

Chloride

The buffering capacity of blood is maintained by a reversible exchange process between bicarbonate and:

1 hour

Semen should be received within

Formation of a fibrin clot

What is the ultimate goal of secondary hemostasis?

*Prothrombin Time (PT) *Activated Partial Thromboplastin Time (APTT) *Russel Viper Venom Test (dRVVT)

What tests will be abnormal in a patient with Stuart-Prower Factor deficiency?

Pernicious anemia

What would the following Schilling test results indicate: Part I: 2% excretion of radioactive vitamin B12 (normal: 5-35%) Part II: 8% excretion of radioactive vitamin B12 after intrinsic factor was given with vitamin B12 (normal: 7-10%).

Just before the next scheduled dose

When is a trough blood sample for therapeutic drug monitoring usually obtained?

If you suspect a cold antibody

When should a prewarm technique be performed?

Chromosome 11

Which chromosome demonstrates a partial or full gene loci deletion in various forms of beta thalassemia?

polarized light

Which is the best method for examination of synovial crystals:

Silent carrier

Which of the following alpha thalassemia syndromes has the genotype -α/αα ?

anti-Jka, Jkb, C, and E

Which of the following antibodies usually show enhanced agglutination with the use of proteolytic enzymes?

Density of a solution compared to density of water

Which of the following best defines specific gravity:

Bind with antigens

Which of the following best describes the primary function of antibodies:

Systolic blood pressure should be no higher than 180, which eliminates candidate #2. Diastolic pressure should be no higher than 100, which eliminates candidate #5. Pulse should be between 50 and 100 beats/minute, which eliminates candidate #1. Temperature above 37.5oC (99.5oF) requires that the donor be temporarily rejected, which eliminates candidate #4. The only candidate that meets all of the requirements for blood donation is candidate #3.

Which of the following candidates would be an acceptable blood donor? Blood pressure / Pulse / Temperature

Cystic Fibrosis

Which of the following disorders is characterized by increased production of chloride in sweat?

An extension cord used in a healthcare facility can be either a two-prong or three-prong cord.

Which of the following is a FALSE statement regarding the use of extension cords in a health care facility?

Leukocyte count greater than 1000 WBC/uL

Which of the following is characteristic of an exudates effusion?

Fibrinogen (consumed during clotting process)

Which of the following is found in plasma but absent in serum:

Osmolality

Which of the following is not a standard method for measuring the specific gravity of urine?

B lymphocytes

Which of the following is responsible for humoral response:

immunoassay

Which of the following is the most common technique that is used by clinical laboratories for therapeutic drug monitoring?

37 degrees Celsius

Which of the following is the proper temperature to use when crossmatching in the presence of a cold antibody:

triglyceride

Which of the following lipid results would be expected to be FALSELY elevated on a serum specimen from a non-fasting patient?

Cholesterol

Which of the following lipid tests is LEAST affected by the fasting status of the patient?

Darkfield microscopy

Which of the following microscopic techniques is best suited for direct examination of the infectious agent of syphilis?

Clostridium perfringens

Which of the following organisms is most likely to be associated with gas gangrene:

*Differential smear Touch prep *Coverslip smear *Pull prep *T-prep (Coverslips, differential smears, pull preps and t-preps are all techniques used when making bone marrow aspirate smears. Touch preps are made from bone marrow biopsy samples. )

Which of the following smear techniques can be utilized when processing bone marrow aspirate samples?

Negative test for fibrin split products

Which of the following test results are NOT characteristic of disseminated intravascular coagulation (DIC)?

Lymphocytes

Which of the following white blood cells would NOT produce a positive leukocyte esterase test on the urine chemical reagent strip? (granulocyte - basophil, eosinophil, neutrophil)

Lymphocytes

Which of the following white blood cells would NOT produce a positive leukocyte esterase test? (Basophils, Eosinophils, Neutrophils)

Small dense LDL molecules are more atherogenic because they can more easily move into the endothelium and vessel wall

Which statement best describes small dense LDL particles that can occur in atherogenic dyslipidemia?

*fish tapeworm *folic acid deficiency

Which two of the following are associated with macrocytic anemia?

Triple Phosphate

"Coffin lid" appearance

Kidd system antibodies *Of the causes that are listed, the most likely cause of a delayed hemolytic reaction is Kidd system antibodies. Both jka and jkb are often responsible for delayed hemolytic transfusion reactions. *Volume overload may cause an immediate, nonimmunological reaction. *Iron overload may cause a delayed, nonimmunological reaction. *ABO incompatibility would cause an immediate immunological reaction.

A delayed hemolytic reaction occurring a week later is MOST likely caused by:

Plasma proteins

A patient experiences a mild allergic reaction to a transfusion, including urticaria, erythema (skin redness), and itching. What is the most likely source of the allergen?

Candida albicans

A potentially pathogenic yeast that is usually found as normal flora in the oropharyngeal cavity and may produce thrush is called:

Hemolytic Disease of the Newborn

A spectrophotometric scan of amniotic fluid may be valuable in the determination of which of the following conditions:

*D *D is the correct answer since the biochemical results are consistent with Shigella sonnei that agglutinates in Group D antisera. Shigella Groups A, B, and C are ornithine negative and usually are indole positive.

A stool from a two-year-old girl grew clear colonies on MacConkey agar, red colonies on XLD, and green colonies on HE without black centers. It was ornithine positive and indole negative. It should agglutinate in antisera of which serogroup?*

lyse the red blood cells

A technologist is having trouble differentiating between red blood cells, oil droplets and yeast cells on a urine microscopy. Acetic acid should be added to the sediment to:

Watson-Schwartz Test

A test that can be used as a confirmatory test for urobilinogen is:

Satelliting "cross match stick" or "sage brush" colonies

A yeast identification system gave a biotype number for an unknown isolate that did not differentiate between Candida tropicalis and Candida parapsilosis. This isolate could be identified as C. parapsilosis in a cornmeal agar preparation if it produced:

Coccidioides immitis

Although care should be taken when working with all fungus cultures in the laboratory, personnel are particularly prone to develop laboratory acquired infections from the inhalation of airborne species of:

*Transfusion of ABO incompatible blood. *Exposure to a red cell antigen when the corresponding antibody is present.

An acute hemolytic reaction may be caused by which of the following?

Bordetella parapertussis

An aerobic gram negative coccobacillus was isolated from a nasopharyngeal swab 48 hours after culture from a 6 month old with the following culture characteristics on Bordet-Gengou agar: *Small zones of beta-hemolysis *Urease: positive *Oxidase: negative *Motility: negative The MOST probable identification of this isolate is:

Potassium, Sodium, Chloride, Carbon dioxide

An electrolyte panel (lytes, chem-4) consists of:

30 minutes

An issued unit of blood may be returned to blood bank inventory if it has not been outside a monitored refrigerator for longer than:

hyperliproteinemia 4

An obese adult with premature arteriosclerosis is seen in the clinic. When her serum is tested no chylomicrons are present, LDL are normal and VLDL are increased. There is an increase in triglycerides and slight increase in cholesterol. Lipoprotein electrophoresis reveals a heavy pre-beta band. She has no skin rash and uric acid is increased. This patient has a hyperlipoproteinemia with the MOST likely type of:

2/3

CSF glucose: Serum glucose

cylindrical, contain Tamm-Horsefall mucoprotein

Casts characteristics

Cystine

Hexagon

*an abrupt rise in LH 24-36 hours prior to ovulation The LH surge is required for ovulation. In fact, 24-36 hours after the Luteinizing Hormone surge, the follicle releases an ovum, which is called ovulation. After ovulation, the corpus luteum produces large amounts of progesterone while estrogen levels are also increased in order to prepare the uterus for a possible pregnancy.

Hormonal variation throughout the menstrual cycle typically shows:

Report the isolate as Beta streptococcus Group B

If a CAMP test had a arrowhead appearance, the bacitracin disk had a zone of 6 mm with a 6 mm disk, the bile esculin was tan throughout, and the SXT disk had a zone of 6 mm with a 6 mm disk, the action you should take would be:

haptens

Incomplete antigen

Bart's

Newborns who will develop Hemoglobin H disease may demonstrate up to 30% of which type of hemoglobin?

Acute glomerulonephritis

Oliguria is usually correlated with:

opsonins

Phagocytosis by reticuloendothelial cells is greatly enhanced by:

negatively; anode

Proteins in a buffer that sets the pH at 8.6 will become ___________ charged and migrate to the ____________.

Howell-Jolly bodies

Remnants of erythrocyte nuclei, nuclear fragments, or aggregates of chromosomes are called:

*tapered *rounded

The ends of rod-shaped bacteria may appear:

*169 mg/dL *The eAG for a HbA1C of 7.5% would be reported as 169 mg/dL eAG. Remember, the formula for conversion of HbA1C to glucose in mg/dL is eAG = (28.7 x A1C) - 46.7. So, in this case, the calculation is: eAG = (28.7 x 7.5) - 46.7 = 168.55 mg/dL.

The formula for conversion of HbA1C to glucose in mg/dL is eAG = (28.7 x A1C) - 46.7. The HbA1C measured on a patient is reported as 7.5%. What would be reported as the estimated average glucose (eAG) for this % A1C (rounded to the nearest whole number)?

Hb S migrates with D in alkaline and alone in acid electrophoresis.

The gel electrophoresis pattern for hemoglobin S shows which of the following migration patterns?

Delta-beta thalassemia minor

The hemoglobin electrophoresis pattern for patient #2 shows an increase in Hb F and a decrease in both Hb A and Hb A2. These results correlate best with which of these thalassemias?

Giant lysosomal granules

The morphologic characteristic(s) associated with the Chediak-Higashi syndrome is (are):

Hemolysis

The most likely cause of an elevated serum potassium level in an apparently normal individual is:

normal erythrocyte

The nucleus of a small lymphocyte is about the same size as a:

Calcium and Phosphate

The parathyroid hormone is important in the regulation of:

Valine

To produce hemoglobin S, glutamic acid that is normally present in the sixth position on the beta globin chain is substituted with which of the following?

Increased serum amylase and normal to low urine amylase values

The results which would be MOST consistent with macro-amylasemia are?

properties of the membrane used

The selectivity of an ion-selective electrode is determined by the:

inhibition

The susceptibility of a bacterium to each drug on a sensitivity plate using antibiotic disks is demonstrated by the diameter of the zone of:

Hemophilus influenzae *HACEK refers to a group of gram-negative bacilli including: Haemophilus species (Haemophilus parainfluenzae, Haemophilus aphrophilus, Haemophilus paraphrophilus), Actinobacillus actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, and Kingella species. Each organism is capable of causing endocardial infections. HACEK organisms are responsible for approximately 3% of cases of infective endocarditis.

The term "HACEK" is often associated with infective endocarditis. Which of the following organisms is NOT included in the "HACEK" group?

Class C

What are fires called that involve electricity?

Stomatocyte

What are the abnormal red blood cells (RBCs) indicated by the arrows that may be suggestive of an individual possessing the Rh null phenotype?

At birth, ABO antibodies are generall absent. ABO antibody production begins at about 3 - 6 months of age.

What is the explanation for a discrepancy in the following front type and back type in a newborn?

1.5

What is the lower reference limit for semen volume according to the World Health Organization, WHO Laboratory Manual for the Examination and Processing of Human Semen, 5th edition?

*8.4 M molarity =moles of solute/liter of solution

What is the molarity of H2SO4 (GMW = 98) that has a specific gravity of 1.21 and an assay weight of 68% w/v?

30 minutes

What is the optimal time to begin initial microscopic examination of a semen specimen, assuming liquefaction is complete?

Decolorize all gram negative organisms

What is the purpose of using alcohol in the gram stain procedure:

*Both disk diffusion and broth microdilution tests should be incubated a full 24 hours to detect vancomycin resistance. *BHI agar with 6 ug/mL vancomycin can be employed as a screening methodology for vancomycin resistance. *Methodologies employed should also address the detection of high level resistance to gentamicin and streptomycin.

With regards to identifying resistance in enterococci, which general statements are true?

Lewis

_____ antigens are not a component of the red cell membrane and, in fact, are not produced by the red cell at all. They are adsorbed from the plasma

Urobilinogen

based on Erlich-aldehyde reaction