ASCP Histology Exam Section 7

How many grams of K2(SO4) are required to prepare 500 mL of a 0.5 solution? (M.W.s are K=39, S=32, )=16)

22.75

Which of the following reagents is added to formaldehyde stock solution to prevent polymerization?

Methyl alcohol

Where in the immunohistochemistry (IHC) process are blocking serums utilized?

As an initial block prior to application of primary antibody

Bloody tissues are most prone to the following microtomy artifacts EXCEPT for which one?

Bubbles

NAACLS requires that an approved Histotechnology Program include objectives and methods of evaluation from the following domains:

Cognitive, psychomotor and affective

A pathologist observing a liver section under the microscope sees squamous debris. What could be a reason for this?

Contamination from another tissue

Cerebral spinal fluid (CSF) specimens should be processed using:

Cytospin, ThinPrep or SurePath TM

Rotary microtome knives/blades used paraffin sectioning are usually made f what material?

Stainless steel

For a histochemical procedure, 500mL of TRIS buffer is needed. The most accurate choice of calibrated glassware for preparing the solution is a/an:

Volumetric flask

Upon examination of a section of small intestine under the microscope, cell shrinkage and a lack of surface epithelium is observed. Incomplete fixation is suspected, so what steps should be taken next by the histotechnician?

1. Increase the time the tissue is in fixature solution. 2. Change to another suitable reagent that fixes tissue more rapidly. Both A and D are proper actions