Aseptic Technique - Microbiology

the high power objective magnifies

40 times so that is 400 with the eyepiece

Aseptic Technique is ?

A fundamental and important laboratory skill in the field of microbiology. Microbiologists use aseptic technique for a variety of procedures such as transferring cultures, inoculating media, isolation of pure cultures, and for performing microbiological tests.

Agar

A gelationous substance obtained from various kinds, used in media as a thicker in foods.

Safety on Culture

All culture media or trash that has come in contact with love bacteria or body fluids into the bio-hazard waste containers.

Bacterial Motility is

Chemotaxity and true motility is achieved by rotation of flagella.

Some inoculation techniques separate bacterial cells to form individual ?

Colonies

Nutrient Agar

Contains Nutrients that support growth of bacteria. Has a P.H. of 6.8 that is acidic

6 I's of Inoculation

Inoculation, Incubation, Isolation, Inspection, Information gathering, identifictation

Ubiquitous

It means that it is present , it appears or can be found everywhere.

During inoculation what instruments are used?

Loops and needles

Safety on Spills and Chemicals

Make sure to report all spills, even water. Keep containers away from edge of workbench. All Chemicals will stain clothing and tarnish jewelry.

Safety Question

Never taste a chemical. Only wafting of vapors towards your nose with your hands is allowed when told so by the instructor

Use ONLY fine adjustment to focus when using high power or oil.

Never use the coarse adjustment.

Why is aseptic technique necessary/important?

To keep infectious microorganism from sterile surfaces or tissues. Helps prevent contamination of test and or experiments in a lab.

Safety Question Bunsen and Burns

Turn off bunsen burner or hot plate when not using. If you get burned you run cold water on burn area for 15 minutes. The cold water reduces pain and blisters.

How do you return the light microscope to the cabinet?

Turn off the light and allow time to cool off. But the scanning objective back into position, coil up the cord properly and cover with the dust cover.

When you enter the lab?

You wash your hands with antibacterial soap. Clean your area with antiseptic spray, and before you leave make sure to wash your hands again with antibacterial soap.

colonies

a group of cells that are growing together

bacterial colonies

are elevated, colorful, sticky and shinny

Gram + bacteria

are stained purple with crystal violet

Gram - bacteria

are stained red with safranin

Electron Microscopes

are used in a high-tech lab where thin samples are scanned using electrons generating a computer image

Fungal colonies

are usually bigger, grow fast, puffy and dry

identify ways to practice aseptic techniques ?

boiling water, using Bunsen burner, autoclave machine, wash hands, sterilize area, don't place items down on a non-sterile surface.

Under the lower power, what adjustment knob do you use?

both, coarse and fine adjustment knobs to focus

how do you see the specimen?

by turning on the light source and center the specimen in the bean of the light.

When carrying the microscope you

carry it with one hand grasping the arm and the other hand underneath the base.

these bacteria are typically treated with penicillin

gram + bacteria

these bacteria are hard to treat even with penicillin

gram - bacteria

Condenser on the light microscope

has no visual on the microscope it's self. it concentrates light source into a cone of light that passes through diaphragm.

Inoculation

introduction of a pathogen or antigen into a living organism. Usually it's a vaccination, immunization, and/or by injection

Media plate

is a petri dish that has agar applied on it.

Growth Medium

is a solid, liquid or semi- solid designed to support the growth of microbes or cells. It contains nutrients that support microbial growth. It uses agar as a solidifying agent. It can be selective due to nutrients, P.H. and antibiotics.

Light microscope

is an instrument that is used to see organisms that are not seen by the naked eye.

Petri-Dish know hows and what to do.

label base using tape. With a sharpie, put your name, the date and what is inside. Always store your dish upside down. (that way the humidity will not enter-fear with your experiment) Seal your dish with parafilm. It is a stretchy tape like material used to seal your dish with.

The red scanning objective

magnifies 4 times plus the eyepiece so 40 times

Be sure to always center the specimen in your field aka the slide, of our view before rotating the

nose piece to change objectives.

The part that you look trough on the top of the microscope is called the

ocular eyepiece and it magnifies 10 times

Sub culturing (such as streak plating and loop dilution) will eventually achieve a

pure culture

When you see a slide for the 1st time you always put the objective on what setting?

scanning objective

the last objective is the oil immersion it magnifies 100 times for 1000 times with the eyepiece

this objective is the hardest to use. Oil immersion will be use to prevent the light from scattering and able to see the objective better.

Broth in Media inoculation technique is used

to grow large numbers quickly

Use a loop when ..

transferring to a broth

Compound Microscopes

use one magnifying lens and light and magnifies up to 1000 times

Antagonism

when one organism prevents other organisms from growing in its vacenaty

What is streak plating?

when you drag from one culture to another making a "z" looking motion.

How do you clean the lens?

with lens paper/cleaning fluid

the low power objective magnifies

10 times so 100 times with eyepiece

What technique is used to achieve isolation?

Streak plate