bio 101 chap 14

which of the following are applications of transgenic bacteria?

-production of insulin -clean up of oil spills production of growth hormones

genomics seek to determine ___.

-sequences of introns and exons in certain genes of certain organism -sequences of DNA and the number if genes present in organism

select the choices that may refer to a plasmid

-vector prokarryotic DNA

PCR is a technque used to create copies of a segment of ___ quickly in a test tube.

DNA

recombinant DNA (rDNA) is

DNA from two or more differnet sources

DNA gel electorphoresis is able to ___ DNA fragments.

Sort

5th step in the production of recombinant DNA

allow vector to reprodue to clone the gene.

the process that is used to separate DNA fragments according to saize is ___.

called gel electrophoresls

3 step in the production of recombinant DNA

cleave the cDNA and vector DNA with same restriction enzyme

the production of genetically identical copies of DNA, cells, or organisms through some a sexual means is called ___

cloning

regions of a gene that will eventually become translated into protenins

exons

a technology that can identify and distinguish among indivilduads based on variation of DNA is called DNA___

fingerprinting

the type of cloning throught which identical copies of a functional unit if DNA are made is called ___ cloning.

gene

a scientist seeking to study the complete genetic makeup of an organism is ctudying in the field of

genomics

the ___ project sought to determain the complete sequence of human DNA

human genome

first step in the production of recombinant DNA

identify and mRNA from a target gene

regions of the genome located between genes

intergenic sequernces

4th step in the production of recombinant DNA

introduce DNA ligaes to connect sticky ends of DNA

regions of a gene that will be ezcised after transcription

intron

DNA ____ is an enzyme that links DAN fragments together

ligase

DNA is cut with___ enzymes at specitic points during recombinant DNA production.

restriction

genomics is ___.

the study of genomes

which of the folloing happens first in the process of recombinant DAN production?

the target DNA and vector are treated with the same restriction enzyme

which of the following happens first in the process of recombinant DNA production>

the target dna and vector are treated with the same restriction

gene ____ is a correction of detrimental DNA mutation by inserting new DNA into the genome

therapy

why dose the DNA polymerase used for PCR need to come from the organism therus aquatics??

this organism produces a DNA polymerase that can withstand heat.

genetically modified organisms that have had foreign DNA inserted into their genome are said to be ___ organisms.

transgenic

true or false; modern science has been able to successfully clone a mammal

true

ture of false: in humnas aaround 95% if a protein coding gene is actually made up of introns

true.

2 step in the production of recombinant DNA

use reverse transcription to produce a complementary DNA