bio 101 chap 14
which of the following are applications of transgenic bacteria?
-production of insulin -clean up of oil spills production of growth hormones
genomics seek to determine ___.
-sequences of introns and exons in certain genes of certain organism -sequences of DNA and the number if genes present in organism
select the choices that may refer to a plasmid
-vector prokarryotic DNA
PCR is a technque used to create copies of a segment of ___ quickly in a test tube.
DNA
recombinant DNA (rDNA) is
DNA from two or more differnet sources
DNA gel electorphoresis is able to ___ DNA fragments.
Sort
5th step in the production of recombinant DNA
allow vector to reprodue to clone the gene.
the process that is used to separate DNA fragments according to saize is ___.
called gel electrophoresls
3 step in the production of recombinant DNA
cleave the cDNA and vector DNA with same restriction enzyme
the production of genetically identical copies of DNA, cells, or organisms through some a sexual means is called ___
cloning
regions of a gene that will eventually become translated into protenins
exons
a technology that can identify and distinguish among indivilduads based on variation of DNA is called DNA___
fingerprinting
the type of cloning throught which identical copies of a functional unit if DNA are made is called ___ cloning.
gene
a scientist seeking to study the complete genetic makeup of an organism is ctudying in the field of
genomics
the ___ project sought to determain the complete sequence of human DNA
human genome
first step in the production of recombinant DNA
identify and mRNA from a target gene
regions of the genome located between genes
intergenic sequernces
4th step in the production of recombinant DNA
introduce DNA ligaes to connect sticky ends of DNA
regions of a gene that will be ezcised after transcription
intron
DNA ____ is an enzyme that links DAN fragments together
ligase
DNA is cut with___ enzymes at specitic points during recombinant DNA production.
restriction
genomics is ___.
the study of genomes
which of the folloing happens first in the process of recombinant DAN production?
the target DNA and vector are treated with the same restriction enzyme
which of the following happens first in the process of recombinant DNA production>
the target dna and vector are treated with the same restriction
gene ____ is a correction of detrimental DNA mutation by inserting new DNA into the genome
therapy
why dose the DNA polymerase used for PCR need to come from the organism therus aquatics??
this organism produces a DNA polymerase that can withstand heat.
genetically modified organisms that have had foreign DNA inserted into their genome are said to be ___ organisms.
transgenic
true or false; modern science has been able to successfully clone a mammal
true
ture of false: in humnas aaround 95% if a protein coding gene is actually made up of introns
true.
2 step in the production of recombinant DNA
use reverse transcription to produce a complementary DNA