BIO 111 Lab examine 1

Which of the following are not allowed in lab? More than one answer may be correct. - Eating food - Applying cosmetics (including lip balm) - Drinking water (or anything else) - Chewing gum

- Eating food - Applying cosmetics (including lip balm) - Drinking water (or anything else) - Chewing gum

Which of the following statements are TRUE regarding BIOL 111 labs? - Reapplying cosmetics like Chapstick inside the lab is acceptable because you are not actually eating anything. - Gloves are required for some experiments. - Drinking water is permitted as long as the water bottle remains sealed when not in use. - Never eat in lab (includes candy and chewing gum).

- Gloves are required for some experiments. - Never eat in lab (includes candy and chewing gum).

Which of the following are good routine practices to maintain safety in the lab? - Using a closable sippy cup for your water while in lab. - Keeping food and drink out of the lab or stored securely inside a backpack. - Placing backpack and bags in the cubby or on the hook in the knee hole. - Pushing your lab stool in under the desk when not in use.

- Keeping food and drink out of the lab or stored securely inside a backpack. - Placing backpack and bags in the cubby or on the hook in the knee hole. - Pushing your lab stool in under the desk when not in use.

Which of the following items are required for this week's lab? - Long Hair Tied Back - Lab Coat - Gloves - Safety Goggles - Closed Toe Shoes - Face Shield

- Long Hair Tied Back - Gloves - Safety Goggles - Closed Toe Shoes

Which of the following are good routine practices to maintain safety in the lab? - Placing backpack and bags in the cubby or on the hook in the knee hole. - Pushing your lab stool in under the desk when not in use. - Keeping food and drink out of the lab or stored securely inside a backpack. - Using a closable sippy cup for your water while in lab.

- Placing backpack and bags in the cubby or on the hook in the knee hole. - Pushing your lab stool in under the desk when not in use. - Keeping food and drink out of the lab or stored securely inside a backpack.

Which of these statements are true about micropipettors? More than one answer may be correct. - The second stop is used to dispense fluid from the tip. - The first stop is used to draw the desired volume into the tip. - Have a specific range of volumes which each size micropipette can handle. - Dispense very small and precise volumes of fluid.

- The second stop is used to dispense fluid from the tip. - The first stop is used to draw the desired volume into the tip. - Have a specific range of volumes which each size micropipette can handle. - Dispense very small and precise volumes of fluid.

Which of the following are true about spectrophotometers? - Works by shining light of a specific wavelength through the sample. - Measures the mass of solutes in a solution. - Measures how much light is absorbed by a solution. - Measures how much light passes through the sample (transmittance).

- Works by shining light of a specific wavelength through the sample. - Measures how much light is absorbed by a solution. - Measures how much light passes through the sample (transmittance).

If you have a sugar with a molecular weight of 51 g/mol, how many grams would you need to add to 47 mL of water to create a 43 mM solution?

0.103

Match the following components to their role in the experiment. 1. Dry beans 2. Soaked beans 3. Water bath 4. Respirometer

1. Control 2. Experimental sample 3. Keeps samples at a constant temperature 4. A tool to measure the consumption of gas

Place the following steps of aerobic respiration in order.

1. Glycolysis 2. Oxidation of pyruvate 3. Krebs/citric acid cycle 4. Electron transport chain

1. A measure of mass. 2. A graph where properties of known samples are plotted. 3. How close a result is to the correct/standard value. 4. How variable a measurement is (for example, if you are measuring the same value on three occasions).

1. Molar Weight 2. Standard Curve 3. Accuracy 4. Precision

Place the steps of measuring a sample's absorbance in order from 1-5.

1. Set wavelength on spectrophotometer. 2. Wipe the cuvette containing the blank and insert it into the spectrophotometer. 3. Adjust absorbance/transmittance dials till the readings are 0. (Then remove cuvette.) 4. Wipe the cuvette containing the sample and insert it into the spectrophotometer. 5. Record absorbance reading.

In spectrophotometry, [1] is the fraction of light that passes through the sample while [2] is the fraction of light that does not pass through the sample.

1. Transmittance 2. Absorbance

Select the appropriate pipette to use for each of the following volumes. 15 57 340 874

15 use a P20 57 use a P200 340 use a P1000 874 use a P1000

A portion of something.

Aliquot

Which of the following statements about cellular respiration are TRUE? More than one answer may be correct. - Aerobic respiration is advantageous because it does not require the presence of O2. - Anaerobic respiration utilizes NAD+ as the final electron acceptor. - Fermentation is most commonly utilized when cells have plenty of O2 available. - Anaerobic respiration produces fewer ATP than aerobic respiration.

Anaerobic respiration produces fewer ATP than aerobic respiration.

Which of the following would not be expected to have an effect on the activity of ß-galactosidase? More than one answer may be correct. BSA Concentration Temperature ß-galactosidase pH ONPG (ortho-nitrophenol-ß-galactoside) concentration Absorbance

BSA Concentration Absorbance

Which of these reagents binds to protein to create a color change that you can measure? in lab 2 NaCl (Sodium Chloride) Bovine Serum Albumin Spectrophotometer Bradford Reagent

Bradford Reagent

What is the purpose of lysis buffer in the experimental reactions?

Breaks open cells by disrupting the cell membrane.

Which microorganism will be used in this week's experiments? Lab 5: Chlamydomonas Escherichia coli Galactosidase Sodiumazidas

Chlamydomonas

Ortho-nitrophenol production can be measured with a spectrophotometer due to which of the following characteristics? - Mass - Temperature - pH - Color

Color

Amira, Jon, Brit and K'Shawn are all in the same lecture class together. The professor occasionally posts extra credit questions in class only for those in physical attendance. One day, Jon and K'Shawn decide to skip class to work on a chemisty assignment at home. During that day's class, the professor posts a bonus point question. Brit texts the access code to Jon and K'Shawn via their group chat. Which of the following types of academic dishonesty did Brit commit?

Complicity

The tubes which hold the sample inside the spectrophotometer.

Cuvette

Select the wavelengths that will be tested for their effect on photosynthesis. More than one answer may be correct. X-rays Green light Ultraviolet light White light Red light Blue light

Green light White light Red light Blue light

An instrument to measure and deliver very small volumes with high accuracy and precision.

Micropipette

James struggled in his first semester and ended up Q-dropping his BIOL111 class. He seems to have things turned around and is taking BIOL111 again in the spring semester. James did pretty well in lab his first time through, so when lab starts up, he just submits his old lab reports that he scored well on instead of redoing the assignment. Which of the following types of academic dishonesty did James commit?

Multiple submissions

During the process of glycolysis, the potential chemical energy released by the breakdown of glucose is stored as - ADP and NAD+ - NADH and ATP - CO2 and H2O - Pyruvate

NADH and ATP

Photosynthesis uses, releases, and produces different molecules. Use/release/production of which of the following causes the floating spinach disks measured in the photosynthesis and wavelength experiment? DCMU Glucose CO2 O2

O2

Bethany is working on her two-page summary for her lab report but is really crunched for time due to a biology lecture exam and another exam in chemistry. She notices that there are a number of old lab reports available online. She decides to synthesize a lab report by cutting bits out of other's old reports and wordsmithing them together. She then submits her report on time. Which of the following types of academic dishonesty did Bethany commit?

Plagiarism

Which of the following materials from this week's lab require special safety consideration (be more careful with them than normal!)? More than one answer may be correct. Iodine Chlamydomonas Sodium azide DCMU

Sodium azide DCMU

An instrument for measuring the amount of light that passes/does not pass through a sample.

Spectrophotometer

Which are required for photosynthesis to occur? More than one answer may be correct. Oxygen Sunlight Water Carbon dioxide

Sunlight Water Carbon dioxide

Which microorganism will be used in the fermentation experiments? Lab 4: Bacteria Yeast Protozoa Mold

Yeast

When creating a serial dilution from 1/10 to 1/1000, the first dilution will be [a] part sample and [b] parts diluent (what you dilute the sample in). The second dilution will be one part [c] and nine parts [d]. The final dilution will be one part [e] and nine parts diluent.

a. 1 (one) b. 9 (nine) c. 1/10 dilution d. Diluent e. 1/100 dilution

In general, a slight increase in temperature will [a] the rate of cellular respiration. This is most directly due to [b] increasing in [c] conditions.

a. Accelerate b. Enzyme activity c. Warmer

[a] is an overarching term that includes all processes living organisms use to produce [b].

a. Cellular Respiration b. ATP

Experiment 1 this week will examine the effect which substrate [a] has on enzyme [b]. Experiment 2 will instead vary [c] concentration. Both experiments will utilize the [d] to measure changes in [e].

a. Concentration b. Activity c. Enyzme d. Spectrophotometer e. Absorbance

Photosynthesis results in the production of [a], which is the primary energy source in cells. The byproduct of photosynthesis is [b].

a. Glucose b. Oxygen

Which of the following statements about the Chlamydomonas experiment are TRUE? More than one answer may be correct. a. Movement of Chlamydomonas will be used to test the effect of metabolic poisons. b. Effect of the metabolic poisons on photosynthesis will be measured using bicarbonate indicator to detect changes in pH. c. Chlamydomonas treated with DCMU will be able to photosynthesize and respire like normal. d. Sodium azide is a metabolic poison inhibits the mitochondrial electron transport chain.

a. Movement of Chlamydomonas will be used to test the effect of metabolic poisons. d. Sodium azide is a metabolic poison inhibits the mitochondrial electron transport chain.

In [a], energy from sunlight is used to extract electrons from [b]. The electrons travel through the [c] to [d], which reduces [e] to [f].

a. Photosystem II b. H2O (Water) c. Electron Transport Chain d. Photosystem I e. NADP+ f. NADPH

Enzymes bind [a], which fit into a spot called the [b]. The enzyme then [c] a biochemical reaction, [d] the rate at which the reaction takes place.

a. Substrates b. Active Site c. Catalyzes d. Speeding Up

In the wavelength and photosynthesis experiment, what is the purpose of the beaker left in the dark? a. The beaker in the dark is a control because it has no spinach discs. b. The beaker in the dark is a control because the spinach will be able to photosynthesize without the influence of selected wavelengths. c. The spinach in the dark is a control because light can interfere with the sodium bicarbonate solution. d. The beaker in the dark is a control because it has all the experiment components but photosynthesis should not occur without light

d. The beaker in the dark is a control because it has all the experiment components but photosynthesis should not occur without light

Match the following terms based on this week's experiments: Lab 3: The colorless product is The product which you will measure via spectrophotometry is The substrate is The enzyme is

galactose ortho-nitrophenol ONPG (ortho-nitrophenyl-beta-galactoside) Beta-galactosidase

The [x] is used when pulling the desired volume into the tip. The [y] is used to dispense the full volume out of the tip

x. First Stop y. Second Stop

When you are doing an experiment, your hands should be covered by [x] and your [y] should be covering your eyes. Goggles which are propped on your [z] and gloves which are still in their [a] will not protect you from chemicals.

x. Gloves y. Goggles a. Box z. Forehead

This week's experiment will examine cellular respiration by measuring [x] consumption. Meanwhile, any [y] produced by the cells will react with KOH (potassium hydroxide) to form non-gaseous molecules.

x. O2 (oxygen) y. CO2 (carbon dioxide)