Biotech SRP Final Exam
Which kind of molecule acts as DNA glue?
DNA Ligase
What is recombinant DNA?
DNA from multiple organisms
What is the correct order of the central dogma of molecular biology?
DNA → RNA → Protein
List the steps of PCR in the correct order
Denaturing, Annealing, Extension
The step of PCR that involves the nucleotides being added to the primers to create a copy of the DNA template is:
Extension
To extract liquid from a solution using a micropipet, go to the
First stop
If you were measuring 2.5 grams of a powder, you would use
a table top balance
What does M stand for (in lab math)?
molar
All of the following are hallmarks of cancer EXCEPT:
neoplasm
Where does transcription occur in eukaryotic cells?
nucleus
What is NOT acceptable in a lab notebook?
opinions
What tool would be suitable for micropipetting 15 microliters?
p100
At what temperature (degrees Celsius) do bacterial plates usually incubate at?
37
How many bases are there in DNA?
4
What is an antigen?
A substance that stimulates production of antibodies
What are the four areas of biotechnology?
Agriculture, Pharmaceutical, Diagnostic/Research, Industrial
What is a potential cause of cancer?
All of the above
Which is a use of rDNA?
All of the above
What binds to an antigen?
Antibody
Where do plasmids naturally occur?
Bacteria
What do you do with a bacteria plate after you're done with it?
Bleach it and throw it away
What is "apoptosis?"
Cell suicide
What are stem cells?
Cells can give rise to cells of any type
What is the word that means a cell is ready to take up foreign DNA
Competent
Which pH is most acidic?
2
How many amino acids are there?
20
What is the concentration of agarose in a typical electrophoresis gel?
1%
What is the basic structure of a virus?
Have genetic material covered by a protein capsule also known as a capsid
Who are HeLa cells named after?
Henrietta Lacks
What is DNA Barcoding?
Identifying a species
Which of the following is not a bacteria?
Influenza
What is a good indicator that science-related news is true?
It comes from a peer-reviewed study and from a credible source
What is a con of cell culture?
Nearly impossible to create the cell's actual environment
Which lab technique multiplies DNA exponentially?
PCR
What does PCR stand for?
Polymerase Check Reaction
What does PA in REE-PE-PA stand for?
Practical Applications
What protein structure level is a sequence of amino acids?
Primary
What should be in a conclusion?
REE, PE, PA
Which kind of molecule cuts DNA?
Restriction Enzymes
What is another name for direct ELISA?
Sandwich ELISA
To expel liquid from a solution using a micropipet, go to the
Second stop
What do you mix agarose powder with when making a gel?
TAE buffer
Which of the following is NOT aseptic technique?
Using the same pipette tips between solutions
What is the correct way to smell the contents of a test tube?
Waft carefully
What is DNA electrophoresis?
a method used to separate DNA strands by their molecular weight
What is positive control?
a sample you know the outcome of before starting
What is bioinformatics?
applying math and computer science to store and analyze biological data
What should be worn when conducting a lab?
eye protection
What does GMO stand for?
genetically modified organism
What does bioethics mean?
the ethics of medical and biological research
