Biotech SRP Final Exam

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Which kind of molecule acts as DNA glue?

DNA Ligase

What is recombinant DNA?

DNA from multiple organisms

What is the correct order of the central dogma of molecular biology?

DNA → RNA → Protein

List the steps of PCR in the correct order

Denaturing, Annealing, Extension

The step of PCR that involves the nucleotides being added to the primers to create a copy of the DNA template is:

Extension

To extract liquid from a solution using a micropipet, go to the

First stop

If you were measuring 2.5 grams of a powder, you would use

a table top balance

What does M stand for (in lab math)?

molar

All of the following are hallmarks of cancer EXCEPT:

neoplasm

Where does transcription occur in eukaryotic cells?

nucleus

What is NOT acceptable in a lab notebook?

opinions

What tool would be suitable for micropipetting 15 microliters?

p100

At what temperature (degrees Celsius) do bacterial plates usually incubate at?

37

How many bases are there in DNA?

4

What is an antigen?

A substance that stimulates production of antibodies

What are the four areas of biotechnology?

Agriculture, Pharmaceutical, Diagnostic/Research, Industrial

What is a potential cause of cancer?

All of the above

Which is a use of rDNA?

All of the above

What binds to an antigen?

Antibody

Where do plasmids naturally occur?

Bacteria

What do you do with a bacteria plate after you're done with it?

Bleach it and throw it away

What is "apoptosis?"

Cell suicide

What are stem cells?

Cells can give rise to cells of any type

What is the word that means a cell is ready to take up foreign DNA

Competent

Which pH is most acidic?

2

How many amino acids are there?

20

What is the concentration of agarose in a typical electrophoresis gel?

1%

What is the basic structure of a virus?

Have genetic material covered by a protein capsule also known as a capsid

Who are HeLa cells named after?

Henrietta Lacks

What is DNA Barcoding?

Identifying a species

Which of the following is not a bacteria?

Influenza

What is a good indicator that science-related news is true?

It comes from a peer-reviewed study and from a credible source

What is a con of cell culture?

Nearly impossible to create the cell's actual environment

Which lab technique multiplies DNA exponentially?

PCR

What does PCR stand for?

Polymerase Check Reaction

What does PA in REE-PE-PA stand for?

Practical Applications

What protein structure level is a sequence of amino acids?

Primary

What should be in a conclusion?

REE, PE, PA

Which kind of molecule cuts DNA?

Restriction Enzymes

What is another name for direct ELISA?

Sandwich ELISA

To expel liquid from a solution using a micropipet, go to the

Second stop

What do you mix agarose powder with when making a gel?

TAE buffer

Which of the following is NOT aseptic technique?

Using the same pipette tips between solutions

What is the correct way to smell the contents of a test tube?

Waft carefully

What is DNA electrophoresis?

a method used to separate DNA strands by their molecular weight

What is positive control?

a sample you know the outcome of before starting

What is bioinformatics?

applying math and computer science to store and analyze biological data

What should be worn when conducting a lab?

eye protection

What does GMO stand for?

genetically modified organism

What does bioethics mean?

the ethics of medical and biological research


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