Exam 2
Endogenization is a term used to define which of the following statement? A. integration of the proviral dna B. Excision of the proviral C. movement and reintegration of D. integration of the proviral dan into the host chromosomes
A
How is DNA replication of parvovirus and adenovirus similar? A. replication occurs by strand displacement B. they both require protein-linked primers C. a replication fork forms at the origin of replication D. none of the above
A
The mRNAs are synthesized by the Reovirus in which of the following structural state of the virus? A. after the genomic RNAs are released into the cytoplasm from the core B. Double capsid virion stage of the virus after in the acidic endoscope C. when the core particle is released into the cytoplasm D. at the infectious sub viral particle (ISVP) stage after acid-dependent proteolytic cleavage
C
What strategy is used by the poliovirus to make many proteins from a single mRNA? A. alternative translational stop B. alternative splicing C. proteolytic cleavage of the polyprotein D. Alternative translational start
C
Which of the following protein complex is required for the synthesis of RNA primer during SV40 DNA replication? A.DNA pol I and the primase complex B. DNA polymerase b and the primate complex C. DNA polymerase a and the primate complex D. DNA polymerase e and the primate complex
C
which of the following reaction takes place at the splicing site of a pre-mRNA? A. trans-esterification B. methylation C. acetylation D. cis-esterification
A
How many mRNA start sites may be present in an "initiator sequence" among DNA viruses? A. Always 3 B. 1 or multiple C. 1 or 3 D. Always one
B
Spliceosomes which of the following is found in a spliceosome? A. ribosomal RNAs B. small nuclear RNAs (snRNA) C. Micro RNAs (mi-RNA) D. Silencing RNAs (siRNA)
B
Which of the following is incorrect about parvovirus DNA synthesis A. dna replication requires dan pol... B. rep 78/68 proteins are required for RNA primer... C. Replication is continuous D. DNA synthesis is by strand displacement
B
Which of the following statement is NOT correct about Reverse transcription (RT)? A. Prokaryotes, Archea, and Eukaryotes all have RT B. Every virus that contain RT must have integrase to integrate the reverse transcribed DNA into host DNA C. RT was present in our common ancestor before the evolution and separation of the prokaryotes, arches and eukaryotes D. RT might be the bridge between the DNA and the RNA world
B
Which statement about viral DNA synthesis is NOT correct A. small DNA viruses encode at least one protein involved in DNA synthesis B. Some viruses encode all proteins needed for DNA replication C. Large DNA viruses encode many proteins involved in DNA synthesis D. Viral DNA replication is always delayed after infection because it requires the synthesis of at least one viral protein
B
which of the following is incorrect about the poxvirus DNA genome and its replication A. poxvirus DNA is a double stranded DNA with covaneltenly joined B. all the viral replication proteins are synthesized by the virus C. viral replication occurs in the cytoplasm of the cell D. the ends of the poxvirus DNA contain inverted terminal repeats (ITRs)
B
Coronavirus and Retrovirus are two unique viruses among the plus-strand RNA viruses. Which of the following feature make them unique? A. Both a and b are correct B. Both of them carry reverse transcriptase C. They are the only enveloped viruses among plus-stranded RNA viruses D. They are the only plus-stranded RNA viruses with encapsidated genomic RNA in the form of the nucleocapsid
D
Reverse transcriptase is an enzyme with low fidelity and low processivity. Which of the following statement best suites the above definition? A. The enzyme often incorporates wrong nucleotides and its synthesis rate is slow as well B. The enzyme incorporates always correct nucleotides but in doing so the synthesis rate is also slow C. The enzyme often incorporates wrong nucleotides as its synthesis rate is very fast D. The enzyme is always capable of incorporating correct nucleotides at a very high rate of synthesis for a productive viral infection
A
The HIV-1 reverse transcriptase contains p66 protein and p51 proteins and each contain the thumb, finger, and pam domain. which is the most logical way to describe the enzyme? A.heterodimeric protein B. heterotrimeric protein C. homodimeric protein D. homotrimeric protein
A
Which is a part of the poliovirus replication strategy? A. circularization of template for initiation of RNA synthesis B. The production of subgeneric mRNAs C. all of the above D. De novo (without primer) initiation of RNA synthesis
A
Which of the following feature is NOT observed in SV40 large t antigen protein? A. contains pola-primase domain which synthesize RNA primers B. Contain and ATPase domain C. Contain a helices domain which unwinds DNA in association with other proteins D. Contain replication origin binding domain
A
Which of the following process describes the polyadenylation of mRNAs in Vesicular Stomatitis Virus (VSV)? A. Slippage of the RNA polymerase and recopying over a seven residue uridine (U7) at each intergeenic region B. all of the above C. Addition of poly (A) with the help of Poly (A) binding protein, PABP D. Snatching of poly (A) tails from cellular mRNAs
A
Which of the following process facilitates the second template exchange of hepadnavirus reverse transcription? A. a hairpin formation B. template switching is initiated C. RNaseH digestion of the 3' D. Conformational change of TP
A
Which of the following protein is nOT involved in viral DNA replication? A. all of the above are involved B. Helicases C. Exonucleases D. Topoisomerase E. DNA dependent DNA polymerases
A
Which of the following region is the actual start site for mRNA transcription? A. Initiator sequence B. Repressor sequence C. TATA sequence D. Enhancer sequence
A
Which of the following statement is true about adneoviral transcriptional regulation? A. all of the above are true B. E2 produced C. DNA replicaion D. E1A produced in the immediate-early phase
A
Which of the following feature is NOT observed in DNA dependent DNA polymerases? A. Di-valent metal ions coordinate the incoming nucleotide to catalyze the reaction B. They contain the thumb, palm and the finger domain C. The thumb domain act as the catalytic site D. Have conserved GDD or close amino acid motifs at the catalytic site
C
Which of the following is NOT true about retrotransposons? A. They are likely progenitor of retroviruses B. All of the above C. They can be present as LINEs or SINEs D. They are mobile elements of the genome E. They contain retroviral gag pol sequences and code reverse transcriptase
C
Which of the following is NOT true about viral mRNAs? A. All of the above are true B. Viral mRNAs could be genome length or shorter sub-genomic length C. Uncapped viral mRNAs can't be translated into protein by the ribosomes D. Viral mRNAs may be attached with a protein at its 5' end; instead of a cap
C
Which of the following is NOT universal rule of viral dNA replication? A. The dNTPs are incorporated at the 3; OH of the DNA chain B. The DNA synthesis is always template directed C. Replication can initiate at random sites on templates called origin of replication D. DNA synthesis may be primer dependent or independent
C
Which of the following is unique in Reovirus RNA dependent RNA synthesis? A. the mRNAs are produced inside the viral core particle and never synthesized in the cytoplasm B. The RNA depdnet RNA polymerase is located inside the viral core below the turret proteins which synthesize the mRNAs C. All of the above D. One of each of the 10 segments of ds genomic RNA is associated....
C
Which of the following sequence is the part of the "core promoter" sequence for RNA polymerase II? A. Trans-acting local regulatory sequences B. All of the above C. TATA sequenze D. Cis-acting local regulatory sequences
C
Which statement about polyadenylation of DNA virus mRNAs is correct? A. Poly(A) is added at the 5'-end of pre-mRNA B. It always occurs in the cytoplasm C. It occurs after cleavage of pre-mRNA D. Is specified by a stretch of U residues in the template
C
which of the following is NOT true about the second strand (the plus strand) DNA synthesis of a typical retroviral reverse transcripition? A. all of the above B. A polypurine tract (ppp) from RNaseH terminal digestion product serves as the primer for the second strand synthesis C. The first template exchange synthesize the second DNA strand D. The second strand synthesis stops temporarily at the modified base of the tRNA attached to the first strand before template exchange
C
which of the following non-retro-viral DNA replication does not require an RNA primer? A. replication fork based DNA synthesis form a single origin B. replication fork based DNA synthesis from a multiple origins C. strand displacement DNA synthesis D. rolling circular DNA synthesis
C
which of the following steps occur during reverse transcription of retroviral genomic RNA A. priming of (-) DNA synthesis by tRNA B. degradation of the viral RNA by RNaseH C. All of the above D. Two template exchanges E. generation of two lTRs
C
How the "immediate early" transcriptional regulation of Herpesvirus is different from the Adenovirus? A. herpesvirus uses a cascade regulation unlike adenovirus auto regulation B. The herpesvirus immediate early transcription factor VP16 have cellular origin whereas adenovirus packs the immediate early E1A transcription factor in the virion C. Herpesvirus packs its RNA polymerase but Adenovirus do not D. The "immediate early" transcription factor VP16 is packaged into the herpesvirus virion but adenovirus use cellular transcription factor for its immediate early transcription
D
The poliovirus and alpha virus both have (+) strand RNA genome. Which of the following statement describe the difference in their genome and RNA synthesis? A. The poliovirus genome acts B. There is a protein attached at the 5' end C. The poliovirus genome RNA D. All of the above
D
Which of the following enzyme/s has/have conserved GDD amino acid motifs at their catalytic site? A. DNA dependent DNA polymerase B. RNA dependent RNA polymerase C. RNA dependent DNA polymerase D. all of the above E. DNA dependent RNA polymerase
D
Which of the following is NOT true about a typical retrovirus genome and its function? A. The viral genome is coated with nucleocpaid protein B. The retroviral genome is (+) strand RNA with wo identical copies in each virion C. Host tRNAs are packaged in the virion bound to the pos (primer binding site) of the genome D. The genome RNA is 5' capped and 3' polyadenylated and translated into Gag-Pol protein precursor immediately after the release of the mRNA into the cytoplasm
D
Which of the following is the signature structure of a DNA binding domain of a transcriptional activator protein? A. leucine zipper B. dimerization domain C. cloverleaf structure D. zinc finger
D
Which of the following statement is NOT true about the regulation of retroviral dNA integration? A. the BAF B. Preferential C. Integrase function D. All of the above E. Integrase bound to chrom
D
Which statement about viral DNA synthesis is correct? A. some viruses encode all proteins needed for DNA replication B. small DNA viruses do not encode any protein involved in DNA synthesis C. Viral DNA replication begins as soon as the genomic DNA is delivered into the nucleus D. Large DNA viruses encode many proteins involved in DNA synthesis
D