Lab 3 Study Guide

Condenser

Small substage lens that concentrates the light on the specimen

Stage

The microscope slide rests on the ___________ while being viewed.

Stage

The platform the slide rests on while being viewed

Your lab microscope is parfocal. This means that:

The slide should be almost in focus when changing to higher magnifications

Arm

Vertical portion of the microscope connecting the base and head

Base

Supports the microscope

If the ocular lens magnifies a specimen 10X, and the objective lends used magnifies the specimen 35X, what is the total magnification being used to observe the specimen?

350X

Objective lens

Adjustable lens system that permits the use of a scanning lens, a low-power lens, a high power lens, or an oil immersion lens.

You should always begin observation of specimens with the oil immersion lens? True or False

False

Iris diaphragm lever

arm attached to the base of the condensor that regulates the amount of light passing through the condensor. The iris diaphragm permits the best possible contrast when viewing the specimen

Sub-stage light or mirror

located in the base. In microscopes with a sub-stage light source, the light passes directly upward through the microscope: light controls are located on the microscope base. If a mirror is used, light must be reflected from a separate free-standing lamp

Ocular

observations are made through this piece, allowing you to view the specimen

Nosepiece

rotating mechanism at the base of the head. Generally carries three of four objective lens and permits sequential positing of these lens over the light beam passing through them

Head or body tube

supports the objective lens system, which is mounted on a movable nose-piece, and the ocular lens or lenses.

Fine adjustment knob

used for precise focusing once course focusing has been completed

Coarse adjustment knob

used to focus on the specimen

How do you clean the lenses of your microscope?

with isopropyl alcohol and lens paper