MCB 151 Final
List the following steps in order to correctly use a micropipette. -Depress knob through to the second stop --Insert filled tip into Tube B -Insert tipped miropittemen below the surface of liquid (Tube A) -Depress knob to first stop -Slowly release knob to fill tip
5: Depress knob through to the second stop 4: Insert filled tip into Tube B 2: Insert tipped miropittemen below the surface of liquid (Tube A) 1: Depress knob to first stop 3: Slowly release knob to fill tip
What are the three main components of the cytoskeleton?
Actin, intermediate filaments, and mictrotubules.
The functional unit of a cilium or flagellum is the:
Axoneme
What two components are required for photo reactivation repair?
Blue light and photolyase.
In lab 4, how were the restriction enzyme reactions stopped?
Blue loading solution was added to the reaction tubes.
Match the following compound with its function in DNA purification and isolation. Proteinase K, Ethanol, EDTA, SDS (sodium dodecyl sulfate) A. Precipitate the DNA into long fibers that can be collected with a glass rod B. Degrade the proteins that are released after the cell is lysed C. Chelate the divalent cations and protect the exposed DNA from degradation D. Lyse, (break open the cells)
Ehtanol: A Proteinase K: B EDTA: C SDS (sodium dodecyl sulfate): D
Bacteriocidal and bacteriostatic are interchangeable terms.
False
Plasmids are essential for survival of bacterial species?
False
What organells sorts and delivers proteins to different areas of the cell?
Golgi apparatus.
What was the substrate in the beta-galctosidase reaction performed in lab 3?
ONPG (o-nitophenyl-B-D-galactopyranoside)
Review components of muscle tissues.
Okay.
Review types of protozoa.
Okay.
Which one of the following pipets would you use to accurately measure 250 uL? P1000 P10 P1 P20 P200
P1000
After transformation the cells were allowed to incubate in nutrient broth for 30 minutes at 37C. This incubation period was for:
Recovery and Expression of the plasmid DNA
Why is it important to store the plates from this experiment (glow experiment) at room temperature?
Room temperature is the optimal temperature for the lux operon to be expressed.
The functional unit of a muscle cell is the:
Sarcomere
What are the shapes of coccus, bacillus, and spiral bacteria.
Spherical, rod-shaped, twisted.
Terms generally used to describe plasmids.
Supercoiled, Circular, Double stranded, Self replicating
What is the activation energy of a chemical reaction?
The amount of energy required to produce the transition state intermediate.
Why are thymine dimers harmful for the cell?
The dimer forms a distortion of the DNA preventing replication.
What is phr an abbreviation for?
The enzyme photolyase
Cilia are foutnd in both eukaryotic and prokaryotic cells
True
Increasing the B-galactosidase concentration increases the rate of the ONPG hydrolysis reaction.
True
The constituents of the cytoskeleton from largest diameter to smallest diameter are: microtubules > intermediate filaments > actin.
True
What organism is the lux operon from?
Vibrio fischeri
Gel electrophoresis allows you to analyze DNA fragments by separating them based on: i. Shape/Configuration ii. Charge iii. Size iv. AT vs GC content
i, ii, iii
During the Producing a Strain of E. coli that Glows in the dark lab you used which of the following plasmid(s) to confer antibiotic resistance?
pUC18 and Lux
