Microbiology lab enzyme function
In an enzyme controlled reaction, a substrate is the same as
reactant
As the concehtration of an enzyme increases for a given substrate,
reaction rate increases similarly.
The primary function of an enzyme or any biological catalyst
reduced the energy of activation and increase the rate of a reaction.
The optimum condition of an enzyme
Is where it works the best
The shape of an enzyme determines its function
It is true they do
How does an enzyme work to catalyze a reaction?
It supplies the energy to speed up a reaction and lowers the energy barrier needed for reactants to acheive the transition state.
Enzymes are
Proteins
What binds to the active site of an enzyme?
Substrate
What can change the active site of an enzyme and alter its function
Temperature and pH
Everytime you add more enzyme, the reaction rate increases proportionally until the reaction rate suddenly levels off. No other chemicals were added and no modifications were made in the experimental setting. Why did the reaction rate stopped increasing?
The molecules of substrate were all bound to enzyme.
Enzymes have which of the following characteristics?
They are proteins. They can bind with substrate. They act as catalysts.
Enzymes
are specific for their substrates. act as catalysts. have a special shape that controls their function. control the speed of reactions.
Which of the following best describe what enzymes are and how they are used?
catalytic proteins that are efficient in initiating chemical reactions by lowering the activation energy.
Human catalase has an optimum temperature of 37°C. Increasing the temperature by 30 degrees above the optimum range will
denature the enzyme. cause an abrupt decrease in the rate of activity.
When you boil egg white (albumin is protein) you have
denatured the protein
Without undergoing any changes itself, the _____ makes changes to the ______.
enzyme and substrate
When the enzyme is at its most active state, an increase in the concentration of substrate will
not make any difference in the enzyme activity.
Enzymes in human cells tend to perform best
37 degrees Celius
How often can a catalase molecule be used to hydrolyze hydrogen perioxide
Many times
Enzyme activity can be determined by
Measuring how much of the substrate is consumed and measuring how much the product is produce
Is The active site is located on the substrate?
No its is located on the enzyme
Which of the following statements about enzymes is FALSE?
O The substrate binds to the enzyme with low specificity.
