Module 8 Lab Review

What is the time, temperature and psi required for proper sterilization when using an autoclave? a) 10 minutes, 121° F, 10 psi b) 10 minutes, 100° F, 15 psi c) 15 minutes, 100° F, 15 psi d) 15 minutes, 121° F, 15 psi

15 minutes, 121° F, 15 psi

Who discovered the powerful antibiotics called penicillin? a) Alexander Fleming b) Gerhard Domagk c) Louis Pasteur d) Ignaz Sammelweiz e) Joseph Lister

Alexander Fleming

In 1935, he was credited for the discovered the synthetic sulfur chemical, such as sulfonamide, with broad-spectrum antimicrobial properties: a) Alexander Fleming b) Gerhard Domagk c) Louis Pasteur d) Ignaz Semmelweiz e) Joseph Lister

Gerhard Domagk

Who founded the concept of modern antiseptic surgery? a) Alexander Fleming b) Gerhard Domagk c) Louis Pasteur d) Ignaz Sammelweiz e) Joseph Lister

Joseph Lister

Who demonstrated that invisible microbes caused disease? a) Alexander Fleming b) Gerhard Domagk c) Louis Pasteur d) Ignaz Sammelweiz e) Joseph Lister

Louis Pasteur

The comparative standard used to ensure culture standardization in the Kirby Bauer method for determining microbial antibiotic sensitivity and resistance: a) McFarland Standard b) zones of inhibition c) CLSI tables d) Mueller - Hinton

McFarland Standard

Where may infectious organisms be located? a) toys b) door knobs c) telephone d) floors e) all of the above and on everything humans touch

all of the above and on everything humans touch

What is considered to be the source for many of our antibiotics? a) molds such as penicillin b) bacteria such as Actinomycetes c) endospores forming organisms such as Bacillus d) all of the above are sources of antibiotics

all of the above are sources of antibiotics

Which of the following antibiotic modes of action would be less toxic to humans? a) antibiotics that inhibit protein synthesis b) antibiotics that inhibit the production of the cell wall c) antibiotics that disrupt the cell membrane d) all antibiotics are considered to be extremely toxic to humans

antibiotics that inhibit the production of the cell wall

Indicate if the following is considered to be an antiseptic or disinfectant- 70% ethanol a) antiseptic b) disinfectant

antiseptic

Indicate if the following is considered to be an antiseptic or disinfectant- silver nitrate a) antiseptic b) disinfectant

antiseptic

Indicate if the following is considered to be an antiseptic or disinfectant- tincture of iodine a) antiseptic b) disinfectant

antiseptic

Which group of chemicals may be used on the skin? a) antiseptics b) disinfectants

antiseptics

Which method of control is least effective against endospores? a) boiling b) autoclaving c) dry heat d) incineration

boiling

Antibiotics effective against procaryotes which kill or inhibit a wide range of gram-positive and gram-negative bacteria: a) broad spectrum b) narrow spectrum c) limited spectrum

broad spectrum

Which of the following would have the least impact upon the Kirby Bauer method? a) color change b) incubation temperature c) agar thickness d) molecular weight of chemical

color change

Indicate if the following is considered to be an antiseptic or disinfectant- 8% formaldehyde a) antiseptic b) disinfectant

disinfectant

Indicate if the following is considered to be an antiseptic or disinfectant- ethylene oxide gas a) antiseptic b) disinfectant

disinfectant

Of the following, which would most likely compromise the hand-washing technique? a) use of a hand sanitizer following washing with soap b) using a paper towel to turn off the tap after rinsing your hands c) using warm water to rinse your hands d) failure to rub between the fingers and not rubbing the backs of your hands

failure to rub between the fingers and not rubbing the backs of your hands

Which of the following is not considered to be a method or mode of action used to control microbes? a) disruption of the cell membrane b) inhibition of endospore production c) suppression of protein production d) disruption of nucleic acid synthesis e) modification of enzymes

inhibition of endospore production

The primary mode of action for the antibiotic penicillin is: a) inhibits peptidoglycan production b) damages the cytoplasmic membrane c) inhibits translation d) inhibits transcription e) inhibits murine production

inhibits peptidoglycan production

Sterilization: a) only inhibits the growth of bacterial cells b) kills microbial cells but not endospores c) is most easily accomplished by boiling for 10 minutes d) is the complete destruction or elimination of all viable organisms in or on a substance

is the complete destruction or elimination of all viable organisms in or on a substance

Antibiotics only effective against a single organism or disease? a) broad spectrum b) narrow spectrum c) limited spectrum

limited spectrum

Antibiotics effective mainly against of gram-positive or gram-negative bacteria: a) broad spectrum b) narrow spectrum c) limited spectrum

narrow spectrum

If your zone of inhibition is not totally clear, this may indicate: a) sensitivity b) partial sensitivity c) resistance

partial sensitivity

If colonies are seen within the zone of inhibition, what might this indicate? a) control of the microbe b) partial control of the microbes c) presence of mutants

presence of mutants

When observing your plates, how did you know if the chemical effectively controlled the microbe or not? a) the presence and size of a zone of inhibition b) the absence of a zone of inhibition c) fuzzy zone of inhibition

the presence and size of a zone of inhibition

Which of the following seems to be the most effective in the removal of the cold virus from one's hands? a) rinsing with water alone b) washing with soap and water for 20 seconds c) the use of an 85% ethanol hand sanitizer properly d) all of these methods equally remove the virus from one's hands

the use of an 85% ethanol hand sanitizer properly

What does -cidal indicate? a) to halt growth b) to kill c) for use on the skin d) for use on inanimate objects only

to kill

A zone of inhibition must be a certain diameter before considering if the organism is sensitive or resistant to an antibiotic. a) true b) false

true

Antibiotics are substances produced by microorganisms to kill other microbes. a) true b) false

true

Which of the following would remove microbes most effectively from a person's hands? a) rinsing with water only b) washing with soap and water for 10-20 seconds, rubbing between finger and being sure to include the thumbs and back of the hand c) washing with soap and water for 5 seconds and them rinsing with water d) all of the above would equally remove microbes from a person's hands

washing with soap and water for 10-20 seconds, rubbing between finger and being sure to include the thumbs and back of the hand

Of the following, which is the least effective disinfectant? a) bleach b) Lysol c) ammonia d) wood tar distillates such as pine-sol

wood tar distillates such as pine-sol