physio lab 1

what do you expect will happen to the cells exposed to compound X? a. cells die b. no effect c. cells start multiplying d. cells dedifferentiate

a. cells die

the number of dead cells is one of the variables in our experiment. what type of variable is it? a. dependent variable b. independent variable c. random variable d. controlled variable

a. dependent variable

what would be the independent variable? a. number of dead cells b. compound concentration c. number of cells d. experimental control

b. compound concentration

what can you conclude from your initial findings? a. kidney injuries are likely when plasmic concentrations of Ex-P are consumed b. it looks like Ex-P causes kidney injuries c. Ex-P is safe for consumption d. it is proven that kidney injuries are caused by Ex-P

b. it looks like Ex-P causes kidney injuries

what can we conclude about the effect of Ex-P? a. Ex-P does NOT cause kidney injuries b. no conclusion can be made c. Ex-P increases the risk of kidney injuries d. Ex-P is responsible for killing the kidney cells

b. no conclusion can be made

what would be your conclusion if most of the cells in the negative control were red? a. all cells survived as expected b. our experimental set up is flawed c. no conclusion can be made d. the negative control confirms the validity of the experiment

b. our experimental set up is flawed

explore the cells in the microscope and interpret the results. what is your conclusion? (negative control: stained kidney epithelial cells with buffer) a. our experimental set up is flawed b. the negative control confirms the validity of the experiment c. no conclusion can be made d. the experiment needs to be repeated for reproducibility

b. the negative control confirms the validity of the experiment

explore the cells in the microscope and interpret the results. what is your conclusion? (experimental group: stained kidney epithelial cells with plasma solutions A,B,C)what is the approximate percentage of dead cells? a. 90% b. 100% c. 30% d. 1%

c. 30%

what is a hypothesis? a. new theory b. prediction statement c. testable explanation d. uncertain prediction

c. testable explanation

the exposure time was set to 48 hours. what type of variable is the exposure time (and temperature) in this experiment? a. dependent variable b. independent variable c. controlled variable d. random variable

c. controlled variable

explore the cells in the microscope and interpret the results. what is your conclusion? (positive control: stained kidney epithelial cells with compound X) a. no conclusion can be made b. our experimental set up is flawed c. our experimental set up can detect kidney injuries d. this is unexpected

c. our experimental set up can detect kidney injuries

what results are you expecting to see in the negative control? a. all cells turn red b. the cells die c. the cells are not stained d. the majority of cells turn red

c. the cells are not stained

what is the approx. percentage of dead cells? a. 1% b. 10% c. 90% d. 40%

d. 40%

once you have performed the replicates, what are you going to do? a. ask a scientific question b. troubleshoot the experiment c. construct a hypothesis d. analyze data and draw conclusions

d. analyze data and draw conclusions

what needs to be done to prove that Ex-P is responsible for the epidemic? a. perform an experiment with more experimental controls b. present results in a scientific journal c. test Ex-P on large human test groups d. reproduce results with replicates

d. reproduce results with replicates

why do we need to have experimental controls? a. ensure that changes in the dependent variable depend on the independent variable b. ensure the biological variability will not influence the results c. reduce variability in the results d. make the experiment reproducible

a. ensure that changes in the dependent variable depend on the independent variable

What is the most pressing scientific question to address, in order to tackle this public health problem? a. is the kidney toxicity caused by Ex-P?b. how is Ex-P transported to the kidney? c. why are kidney cells the only ones affected? d. what are the molecular mechanisms causing kidney injuries?

a. is the kidney toxicity caused by Ex-p?

there are four cell liens readily available in our lab. which cell line would you use for the experiment? a. kidney epithelial cells b. skin cells c. kidney endothelial cells d. bone marrow cells

a. kidney epithelial cells

have a look at the stained cells. why are the cells stained in pink? a. the pink dye penetrates dead cells b. the pink dye stains cells that were exposed to Ex-P c. the pink dye stains all cells d. the nucleus of pink cells is decomposed

a. the pink dye penetrates dead cells