Quiz Pre-Lab 5: EMB, Mannitol Salt, Streaking for Isolation

What are the characteristics of Enterobacteriaceae? Hint: - G_____-n______ r_____ - Cat_______ p_______ - Produces a_____ from g______ - Ox______ n______

- Gram-negative rods - Catalase positive - Produce acid from glucose - Oxidase negative

Media that contain indicators to expose differences between organisms are referred to as ______ media.

Differential

____ agar contains l_____ and can differentiate between _______ ________ from ___-_________:

EMB Lactose Lactose fermenters and non-fermenters

You inoculate an _____ agar plate with an unknown organism. After incubation, you note while the organism has grown, the growth is colorless. What is your interpretation? Hint: The organism does not ________ ________

EMB The organism does not ferment lactose

E_____ bacteria are h______ p______

Enteric (think ENTERING the body) Human pathogens

This plate was streaked from a pure culture of microorganisms:

False (ask her why)

Mannitol salt agar _______ the growth of organisms that are not in the genus ____________

Inhibits Staphylococcus

Mannitol salt agar contains m_______.

Mannitol

__________ salt agar contains a high concentration of _______ _______

Mannitol Sodium chloride

Media that inhibit the growth of some organisms while encouraging the growth of others are referred to as _______ media.

Selective media

Mannitol salt agar can be used to specifically identify S______ ______.

Staphylococcus

_____ plating is a commonly used technique for isolating i________ species in the microbiology lab.

Streak

You inoculate a ________ salt agar plate with an unknown organism. After incubation, you note that the organism has grown well with c_______ that are y_______ in color and contain a h_____ around them. Which option below best represents the type of bacteria growing on the plate? Hint: Organism inoculated is like s__________ a_______ because there has been _________ of _________.

mannitol, colonies, yellow, halo Organism inoculated is likely Staphylococcus aureus because there has been fermentation of mannitol