Quiz Pre-Lab 5: EMB, Mannitol Salt, Streaking for Isolation
What are the characteristics of Enterobacteriaceae? Hint: - G_____-n______ r_____ - Cat_______ p_______ - Produces a_____ from g______ - Ox______ n______
- Gram-negative rods - Catalase positive - Produce acid from glucose - Oxidase negative
Media that contain indicators to expose differences between organisms are referred to as ______ media.
Differential
____ agar contains l_____ and can differentiate between _______ ________ from ___-_________:
EMB Lactose Lactose fermenters and non-fermenters
You inoculate an _____ agar plate with an unknown organism. After incubation, you note while the organism has grown, the growth is colorless. What is your interpretation? Hint: The organism does not ________ ________
EMB The organism does not ferment lactose
E_____ bacteria are h______ p______
Enteric (think ENTERING the body) Human pathogens
This plate was streaked from a pure culture of microorganisms:
False (ask her why)
Mannitol salt agar _______ the growth of organisms that are not in the genus ____________
Inhibits Staphylococcus
Mannitol salt agar contains m_______.
Mannitol
__________ salt agar contains a high concentration of _______ _______
Mannitol Sodium chloride
Media that inhibit the growth of some organisms while encouraging the growth of others are referred to as _______ media.
Selective media
Mannitol salt agar can be used to specifically identify S______ ______.
Staphylococcus
_____ plating is a commonly used technique for isolating i________ species in the microbiology lab.
Streak
You inoculate a ________ salt agar plate with an unknown organism. After incubation, you note that the organism has grown well with c_______ that are y_______ in color and contain a h_____ around them. Which option below best represents the type of bacteria growing on the plate? Hint: Organism inoculated is like s__________ a_______ because there has been _________ of _________.
mannitol, colonies, yellow, halo Organism inoculated is likely Staphylococcus aureus because there has been fermentation of mannitol
