BIO K101 Quiz 6 & 8 (lab 8, 9, & 10)
During electrophoresis through an agarose gel, DNA migrates towards the
(Red) positive pole
An unfertilized egg of a rabbit contains 22 chromosomes. How many chromosomes are in the somatic (body) cells of a rabbit?
44 (gametes are haploid, n, and somatic are diploid, 2n)
Which of the DNA sequences below is a palindrome? 5' CCAATT 3' 5' CCAAGG 3 5' ATG 3' 5' AGGCCT 3' 5' ACGGATTCGC 3'
5' AGGCCT 3'
A karyotype determined to be 45, XO would indicate
A cell of a female with Turner's syndrome
The region of a chromosome holding the two double strands of replicated DNA together is called __________. a nucleus a chromatid chromatin a centromere a centriole
A centromere
A karyotype determined to be 47, 18+ would indicate
A child with Edward's syndrome
Cytokinesis in animal cells involves contraction of a ring of _____ microfilaments, and cytokinesis in plant cells involves formation of a _________ - centromere, kinetochore - actin, cell plate - microtubules, cell plate - spindle fibers, cell wall
Actin, Cell plate
During prophase, ________________ condense(s) into visible chromosomes. - kinetochores - chromatin - centromeres - centrioles
Chromatin
A cell in G2: - lacks a visible nuclear membrane - has visibly distinct chromosomes - has twice the amount of DNA present in a G1 or a telophase nucleus - is in a dormant, non-dividing state
Has twice the amount of DNA present in a G1 or a telophase nucleus
The M phase of the cell cycle involves two main processes, - meiosis I and meiosis II - interphase and mitosis - mitosis and meiosis - mitosis and cytokinesis
Mitosis and cytokinesis
The ______ is responsible for the separation of the chromosomes during______ of mitosis. - flagellum; metaphase - mitotic spindle; anaphase - centromere; telophase - kinetochore; prophase
Mitotic Spindle; Anaphase
Chromosomes are duplicated during what part of the cell cycle.
S phase
Meiosis II is similar to Mitosis because -Homologous chromosomes separate -Sister chromatids separate -Haploid cells are produced -They both take the same amount of time
Sister chromatids separate
If you discovered a bacterial cell that contained no restriction enzymes, which of the following would you expect to happen? -The cell would be unable to replicate its DNA -The cell would have no chromosomes -The cell would be easily infected and lysed by bacteriophages -The cell would become a pathogen
The cell would be easily infected and lysed by bateriophages
A cell is in metaphase if: - the condensed chromosomes are aligned on the equatorial plane of the cell - the nuclear envelope is clearly visible - the chromosomes are separated into distinct groups at opposite poles of the cell - the chromosomes are visible as thread-like structures
The condensed chromosomes are aligned on the equatorial plane of the cell.
Which of the following events occurs during anaphase of mitosis? Spindle fibers attach to the centromeres of the chromosomes The chromosomes align along the metaphase plate of the cell The nuclear envelope fragments (breaks down) The nuclear envelope re-forms (forms again) The sister chromatids are pulled apart toward opposite sides of the cell
The sister chromatids are pulled apart toward the opposite sides of the cell
The rate of migration of DNA within an agarose gel in the gel electrophoresis technique is primarily based on what factor?
The size of the DNA fragments
How does a bacterial cell protect its own DNA from restriction enzymes?
Through adding methyl groups to adenines and cytosines
What is the enzymatic function of restriction enzymes ?
To cleave nucleic acid at specific sites, often DNA palindromes
Which of the following events occurs during telophase of mitosis? The sister chromatids separate The chromosomes condense Two distinct daughter nuclei form in the cell The chromosomes align on the metaphase plate The nuclear envelope fragments (breaks down)
Two distinct daughter nuclei form in the cell