BIOL 1106 (Final Review)
Write an alternate hypothesis for the change in PH on enzyme activity experiment.
As pH changed so would the rate of the reaction.
When trying to find the unknown agent what could be the different reagents .
Benedict's Iodine Biuret Sudan IV
When we performed experiment in lab then we use 1 : ---------- for proteins 2. ----------------for Sugar 3: ----------------- for lipids
Biuret, Benedicts , Sudan IV
Amino acid can be detected in the food with the
Biurets solution
Pigments are separated in paper chromatography are
Bluish green, Yellow green , Yellow, red
How does a buffer counteract the effect of a strong base?
By donating H+
Staining cells such that their color is different from the background enhances which quality of the cells?
Contrast
What four features are shared by all cells?
DNA, cytoplasm, cell membrane, ribosomes
Write an alternate hypothesis for fermentation experiment.
Depending on the kind of sugar present, yeast ferments at different rates.
The material used in this lab to simulate the permeability of a cell membrane is _________________ tubing.
Dialysis
Electrophoresis buffer is used to create the
Electric field
Gel electrophoresis experiment can be carried out with Distilled water if Buffer is not available to fill the electrophoresis tank.
False
In an experiment of Antacids we use acid drops as an indicator .
False
Iodine solution is used to detect reducing sugar and its color turned purple to red , orange and green accordingly presence of sugars .
False
Mitosis results in half number of chromosome in daughter cells
False
PH and temperature are the only factors that can affect the enzyme activity are
False
Reducing sugar produce color with benedict Reagent but heat is not required for this reaction
False
Test tube with distilled water will be consider positive control for experiment to identify unknown sample ?
False
The terms magnification and resolution can be used interchangeably.
False
When focusing a slide, one should begin with the high power objective and work down to the scanning objective.
False
pH is a measure of ( Concentration of H+) acidity on a scale of 0 through 20.
False
null hypothesis for the fermentation experiment is that
different types of sugar will have no effect on the rate of yeast fermentation.
In this experiment solvent used is
ether and acetone
While examining a cell under a microscope, you observe a nucleus, ribosomes, cytoplasm, and mitochondria. You are examining a _____ cell.
eukaryotic
A high standard deviation means that the data is less spread out.
false
Data set 1 : 1 3 5 7 9 Data set 2: 1 3 5 7 14 In both sets of data mean and median is 5
false
It is possible to make a perfectly precise measurement.
false
Median is the average of a data set when it is arranged from least to greatest.
false
The most common measurement scale for volume is grams.
false
Zero degrees Celsius is the same as 100 degrees Fahrenheit.
false
The portion of a slide that is visible through the eyepiece is called the
field of view
Bright-field microscopes use a combination of
glass lenses and light to view a specimen.
Explain briefly terms hypertonic , hypotonic , isotonic
hypertonic - more solute outside than inside hypotonic - more solute inside than outside isotonic - equal solute inside and outside
What are the independent and dependent variable in this experiment of fermentation ?
independent variable = samples dependent variable = net height change
Cell have two phases interphase and mitotic phase and in these two phases which one is long in duration
interphase
How you will make 50 ml of 0 .8% agrose gel solution for experiment .
0.8grams of agarose to 100mL 400mg (0.4g) of agarose 50X TAE buffer 1mL distilled water 49mL
Given the following data set of measurements made in centimeters of the humerus (upper bone in the forelimb) lengths of a group of bats, what is the median in cm of the data set? 2.51 1.10 5.35 0.79 4.95 1.32 1.82
1.82
When compared to a solution with a pH reading of 2, a solution with a pH value of 3 has ______.
10 times less H+
To make 0.8 % agarose gel in GEL Electrophoresis we need TAE buffer
100 ml
The linear space occupied on your lab tray by the pipets that you will use for lab measures 25 cm long and 5 cm wide. What is the correct way to refer to the area occupied by the pipets?
125 cm2
Given the following data set of measurements made in centimeters of the humerus (upper bone in the forelimb) lengths of a group of bats, what is the variance of the data set? 2.51 1.10 5 .35 0.79 4.95 1.32 1.82
3.47
At the end of cellular respiration One glucose molecule will produce how many ATP produce
32
A microscope has a 4x ocular lens and a 10x objective, what is this microscope's total magnification?
40X
At which magnifications should the fine focus adjustment be use
40X
If the bottom of the meniscus in a 10 ml pipet is at 3 ml, how many ml of liquid is in the pipet?
7
Solvent that is prepared in experiment of pigment separation is
9part ether +1 part acetone
What makes a theory different from a hypothesis? Check all that apply.
A theory has a greater range of experimental support. A theory has stood the test of time.
We checked the PH of different solutions and the null hypothesis for this experiment can be .
All solution have same PH
You have just been given the task of identifying a solution, the content of which is known only by your instructor. All you know about this unknown is that it is either a carbohydrate, a lipid, a protein, or a nucleic acid. You decide to run the unknown through the biochemical tests in this lab. Your results are summarized below. Based on these results, what is the identity of your unknown solution? Test Appearance of solution after the test Benedict's............................deep red Iodine...................................Yellow Biuret...................................light blue Sudan IV.............................Pink
Carbohydrates
What part of all cells encases the cytoplasm and forms a boundary between the cell interior and cell exterior.
Cell membrane
What structure found in plant cells is an organelle for storing water and metabolites?
Central vacuole
What structure is found in plant cells but not in animal cells?
Chloroplast
High energy electron ARE carried to ETC by
FAD and NAD
Carotene in chlorophyll is dark bluish green color.
False
Cellular respiration and Photosynthesis are same metabolic reaction
False
Crossing over is specialized feature of mitosis
False
DNA fragments are positively charged and when placed into an electrophoresis gel will travel through an electrical field towards the respective pole.
False
In an experiment we observe the Temperature changes for enzyme function .Identify the independent and dependent variables for this experiment .
Independent variable - Temperature of Catalase Dependent variable - Bubble volume
Imagine a terrible crime has occurred. Detectives that investigated found a hair at the crime scene that could only have been left by the criminal. DNA analysis took place on the hair, and also on the DNA of the four suspects that were rounded up by the police. You are given the task of deciphering the DNA results, which are listed below. Is there a suspect that you can match to the crime scene? DNA left by the criminal: G-G-T-A-G-G-A-T-C First suspect G-G-A-A-A-C-A-T-C Second suspect: G-G-T-A-G-G-A-C-C Third suspect: G-G-T-A-G-G-T-T-C Fourth suspect: G-G-T-A-G-G-A-T-C Fifth suspect: G-G-T-A-G-C-G-A-C
Fourth suspect
Chemical agent used to detect the starch
Iodine solution
In Photosynthesis atmospheric CO2 is fixed and Glucose is formed and energy required for this process comes from
Light energy
Sudan IV is reagent to identify
Lipids
The total magnification of an image is determined by ______ the magnification power of the ocular lens and the magnification power of the objective lens used.
Multiplying
What causes the DNA segments to move in electrophoresis chamber on gel toward the positive electrode
Negative charge on DNA
When the pH of the stomach becomes too low (too acidic) it can cause pain and damage the stomach lining and it can cause
Stomach ulcer
What does an antacid do to neutralize excess stomach acid?
Take up H+
What is the advantage of starting with the 4X objective before moving to the 10X and then the 40X objective?
The field of view is larger, and it is easier to focus.
If you are testing antacids in lab, you note that the first antacid absorbs 20 drops of HCl before it allows the pH of the solution to change. When you test the second antacid, it takes 7 drops HCl before the pH changes. Which is a more effective antacid?
The first antacid, because it absorbed more acid before allowing a change in pH
How do the coarse and fine focus knobs work on a brightfield microscope?
The focus knobs move the stage up and down
There is a bar graph at the end of fermentation experiment how you will analyze your results?
The glucose has the most net height change while the water has no net height change at all. The most net height change to least are: glucose > fructose > sucrose > starch > water
1: What is dependent variable and what is independent variable . 2: In all antacids different buffers are used . Do all these buffers used to neutralize same acid ?
The independent variable is the antacid and the dependent is the number of drops of acidity or HCI to neutralize the antacid. No not all of these buffers used to neutralize the same acid. The amount of antacids influences their buffer capacity with acid.
Protist are different from Fungi by Select all that apply
The protists are invisible to the naked eye
Write a null hypothesis for change in PH change on enzyme activity experiment.
There will be no difference in enzyme activity between the different pH levels.
Why we used ethyl bromide Dye in electrophoresis gels ?
To see the separated samples under UV light
A cell is the smallest unit of life that can function independently and perform all the activities necessary for life.
True
Best antacid is one that resist the changes in PH longest.
True
Catalase will be used as an enzyme and hydrogen peroxide (H2O2 ) as the substrate.
True
Coarse focus should only be used with the 4X objective.
True
DNA fragments can be separated by size using an electrophoresis gel.
True
DNA fragments run easily in gel when we use Buffer instead of water .
True
During meiosis one cell? divides twice to form four daughter cells.
True
In experiment of dialysis tubing change of strip color shows that glucose is diffused out from dialysis tubing
True
In potato experiment the null hypothesis is all pieces will not change their weight before and after the experiment
True
In this experiment of antacids water was negative control .
True
Increase in PH increase the enzyme activity to certain level
True
Meiosis also produces genetic variation by way of the process of recombination .
True
Meiosis is a feature of Multicellular eukaryote
True
Mitosis can be applied to Organismal growth ,Tissue repair, Asexual reproduction and development
True
Null hypothesis for reducing sugar experiment can be All samples have reducing sugars
True
Osmotic pressure is influenced by changes in nonpermeable solute concentration
True
Parent cell divide two times in meiosis and resulting daughter cells have half number of chromosomes.
True
Purpose of Installing comb into gel is to create well or pockets in the gel where samples can be placed that are required for testing.
True
The Null hypothesis for this experiment can be There will be no difference in heights of students .
True
The more acidic the solution, the lower the pH, and the greater the concentration of hydrogen ions.
True
A photograph of a magnified specimen that is taken through a microscope is called a(n)
micrograph
Molecules that cannot pass through the cell membrane, how moved into or out of the cell
transport proteins channel
Alternate hypothesis can be, Height is more in males than females
true
Condenser critically , regulates the contrast in light microscope .
true
Median is mid value in the ordered data set.
true
Variance measure how far individuals or data points in the group are spread out, in the set of data from the mean.
true
