campbell biology (powell_h) chapter 19

1) Viral genomes vary greatly in size and may include from four genes to several hundred genes. Which of the following viral features is most apt to correlate with the size of the genome?

D) size and shape of the capsid

21) Which of the following series best reflects what we know about how the flu virus moves between species?

E) An animal such as a pig is infected with more than one virus, genetic recombination occurs, the new virus mutates and is passed to a new species such as a bird, the virus mutates and can be transmitted to humans.

12) Which viruses have single-stranded RNA that acts as a template for DNA synthesis?

E) retroviruses

37) You isolate an infectious substance that is capable of causing disease in plants, but you do not know whether the infectious agent is a bacterium, virus, viroid, or prion. You have four methods at your disposal that you can use to analyze the substance in order to determine the nature of the infectious agent. I. treating the substance with nucleases that destroy all nucleic acids and then determining whether it is still infectious II. filtering the substance to remove all elements smaller than what can be easily seen under a light microscope III. culturing the substance by itself on nutritive medium, away from any plant cells IV. treating the sample with proteases that digest all proteins and then determining whether it is still infectious Which treatment could definitively determine whether or not the component is a viroid?

A) I

36) In 1971, David Baltimore described a scheme for classifying viruses based on how the virus produces mRNA. The table below shows the results of testing five viruses for nuclease specificity, the ability of the virus to act as an mRNA, and presence (+) or absence (-) of its own viral polymerase (SEE IMAGE)

A) SEE IMAGE

19) Which of the following is the best predictor of how much damage a virus causes?

A) ability of the infected cell to undergo normal cell division

4) Most human-infecting viruses are maintained in the human population only. However, a zoonosis is a disease that is transmitted from other vertebrates to humans, at least sporadically, without requiring viral mutation. Which of the following is the best example of a zoonosis?

A) rabies

17) The difference between vertical and horizontal transmission of plant viruses is that

A) vertical transmission is transmission of a virus from a parent plant to its progeny, and horizontal transmission is one plant spreading the virus to another plant.

20) Antiviral drugs that have become useful are usually associated with which of the following properties?

B) interference with viral replication

9) Why do RNA viruses appear to have higher rates of mutation?

B) Replication of their genomes does not involve proofreading.

13) What is the function of reverse transcriptase in retroviruses?

B) It uses viral RNA as a template for DNA synthesis.

27) A linear piece of viral DNA of 8 kb can be cut with either of two restriction enzymes (X or Y). These are subjected to electrophoresis and produce the following bands: (SEE IMAGE PART ONE) Cutting the same 8 kb piece with both enzymes together results in bands at 4.0, 2.5, 1.0, and 0.5. Of the possible arrangements of the sites given below, which one is most likely?

B) SEE IMAGE

15) Which of the following describes plant virus infections?

B) They are spread via the plasmodesmata.

16) Which of the following represents a difference between viruses and viroids?

B) Viruses have capsids composed of protein, whereas viroids have no capsids.

2) Viral envelopes can best be analyzed with which of the following techniques?

B) antibodies against specific proteins not found in the host membranes

14) Which of the following can be effective in preventing the onset of viral infection in humans?

B) getting vaccinated

47) RNA viruses require their own supply of certain enzymes because

B) host cells lack enzymes that can replicate the viral genome.

40) The herpes viruses are very important enveloped DNA viruses that cause disease in all vertebrate species and in some invertebrates such as oysters. Some of the human ones are herpes simplex (HSV) I and II, causing facial and genital lesions, and the varicella-zoster (VSV), causing chicken pox and shingles. Each of these three actively infect nervous tissue. Primary infections are fairly mild, but the virus is not then cleared from the host; rather, viral genomes are maintained in cells in a latent phase. The virus can then reactivate, replicate again, and be infectious to others. If scientists are trying to use what they know about HSV to devise a means of protecting other people from being infected, which of the following would have the best chance of lowering the number of new cases of infection?

B) interference with new viral replication in preexisting cases

34) In 1971, David Baltimore described a scheme for classifying viruses based on how the virus produces mRNA. The table below shows the results of testing five viruses for nuclease specificity, the ability of the virus to act as an mRNA, and presence (+) or absence (-) of its own viral polymerase (SEE IMAGE) Given Baltimore's scheme, a positive sense single-stranded RNA virus such as the polio virus would be most closely related to which of the following?

B) retroviruses that require a DNA intermediate

31) Some viruses can be crystallized and their structures analyzed. One such virus is Desmodium, or yellow mottle virus, which infects beans. This is a member of the tymovirus group and has a single-stranded RNA genome of ~6,300 nucleotides. Its virion is 25—30 nm in diameter, and is made up of 180 copies of a single capsid protein that self-associate to form each capsomere, which has icosahedral symmetry with 20 facets. In a cell-free system, what other components would you have to provide for this virus to express its genes?

B) ribosomes, tRNAs, amino acids, and GTP

38) You isolate an infectious substance that is capable of causing disease in plants, but you do not know whether the infectious agent is a bacterium, virus, viroid, or prion. You have four methods at your disposal that you can use to analyze the substance in order to determine the nature of the infectious agent. I. treating the substance with nucleases that destroy all nucleic acids and then determining whether it is still infectious II. filtering the substance to remove all elements smaller than what can be easily seen under a light microscope III. culturing the substance by itself on nutritive medium, away from any plant cells IV. treating the sample with proteases that digest all proteins and then determining whether it is still infectious If you already knew that the infectious agent was either bacterial or viral, which treatment would allow you to distinguish between these two possibilities?

C) III

33) Poliovirus is a positive-sense RNA virus of the picornavirus group. At its 5' end, the RNA genome has a viral protein (VPg) instead of a 5' cap. This is followed by a nontranslated leader sequence, and then a single long protein coding region (~7,000 nucleotides), followed by a poly-A tail. Observations were made that used radioactive amino acid analogues. Short period use of the radioactive amino acids result in labeling of only very long proteins, while longer periods of labeling result in several different short polypeptides. What conclusion is most consistent with the results of the radioactive labeling experiment?

C) The RNA is only translated into a single long polypeptide, which is then cleaved into shorter ones.

8) Which of the following statements describes the lysogenic cycle of lambda (λ) phage?

C) The phage genome replicates along with the host genome.

11) A researcher lyses a cell that contains nucleic acid molecules and capsomeres of tobacco mosaic virus (TMV). The cell contents are left in a covered test tube overnight. The next day this mixture is sprayed on tobacco plants. Which of the following would be expected to occur?

C) The plants would develop the typical symptoms of TMV infection.

42) The herpes viruses are very important enveloped DNA viruses that cause disease in all vertebrate species and in some invertebrates such as oysters. Some of the human ones are herpes simplex (HSV) I and II, causing facial and genital lesions, and the varicella-zoster (VSV), causing chicken pox and shingles. Each of these three actively infect nervous tissue. Primary infections are fairly mild, but the virus is not then cleared from the host; rather, viral genomes are maintained in cells in a latent phase. The virus can then reactivate, replicate again, and be infectious to others. In order to be able to remain latent in an infected live cell, HSV must be able to shut down what process?

C) apoptosis of a virally infected cell

45) To cause a human pandemic, the H5N1 avian flu virus would have to

C) become capable of human-to-human transmission.

6) In many ways, the regulation of the genes of a particular group of viruses will be similar to the regulation of the host genes. Therefore, which of the following would you expect of the genes of the bacteriophage?

C) control of more than one gene in an operon

43) Which of the following characteristics, structures, or processes is common to both bacteria and viruses?

C) genetic material composed of nucleic acid

25) In the figure, at the arrow marked II, what enzyme(s) are being utilized?

C) host cell DNA polymerase

32) Poliovirus is a positive-sense RNA virus of the picornavirus group. At its 5' end, the RNA genome has a viral protein (VPg) instead of a 5' cap. This is followed by a nontranslated leader sequence, and then a single long protein coding region (~7,000 nucleotides), followed by a poly-A tail. Observations were made that used radioactive amino acid analogues. Short period use of the radioactive amino acids result in labeling of only very long proteins, while longer periods of labeling result in several different short polypeptides. What part of the poliovirus would first interact with host cell ribosomes to mediate translation?

C) the VPg protein

30) Some viruses can be crystallized and their structures analyzed. One such virus is Desmodium, or yellow mottle virus, which infects beans. This is a member of the tymovirus group and has a single-stranded RNA genome of ~6,300 nucleotides. Its virion is 25—30 nm in diameter, and is made up of 180 copies of a single capsid protein that self-associate to form each capsomere, which has icosahedral symmetry with 20 facets. If this virus has a positive RNA strand as its genome, it begins the infection by using this strand as mRNA. Therefore, which of the following do you expect to be able to measure?

C) translation rate

28) Some viruses can be crystallized and their structures analyzed. One such virus is Desmodium, or yellow mottle virus, which infects beans. This is a member of the tymovirus group and has a single-stranded RNA genome of ~6,300 nucleotides. Its virion is 25—30 nm in diameter, and is made up of 180 copies of a single capsid protein that self-associate to form each capsomere, which has icosahedral symmetry with 20 facets. If this virus has capsomeres with 20 facets, how many proteins form each one?

C) ~6

7) Which of the following is characteristic of the lytic cycle?

D) A large number of phages are released at a time.

23) Which of the three types of viruses shown above would you expect to include glycoproteins?

D) I and II only

39) You isolate an infectious substance that is capable of causing disease in plants, but you do not know whether the infectious agent is a bacterium, virus, viroid, or prion. You have four methods at your disposal that you can use to analyze the substance in order to determine the nature of the infectious agent. I. treating the substance with nucleases that destroy all nucleic acids and then determining whether it is still infectious II. filtering the substance to remove all elements smaller than what can be easily seen under a light microscope III. culturing the substance by itself on nutritive medium, away from any plant cells IV. treating the sample with proteases that digest all proteins and then determining whether it is still infectious Which treatment would you use to determine if the agent is a prion?

D) IV only

35) In 1971, David Baltimore described a scheme for classifying viruses based on how the virus produces mRNA. The table below shows the results of testing five viruses for nuclease specificity, the ability of the virus to act as an mRNA, and presence (+) or absence (-) of its own viral polymerase (SEE IMAGE) Based on the above table, which virus meets the Baltimore requirements for a retrovirus?

D) SEE IMAGE

46) A bacterium is infected with an experimentally constructed bacteriophage composed of the T2 phage protein coat and T4 phage DNA. The new phages produced would have

D) T4 protein and T4 DNA.

41) The herpes viruses are very important enveloped DNA viruses that cause disease in all vertebrate species and in some invertebrates such as oysters. Some of the human ones are herpes simplex (HSV) I and II, causing facial and genital lesions, and the varicella-zoster (VSV), causing chicken pox and shingles. Each of these three actively infect nervous tissue. Primary infections are fairly mild, but the virus is not then cleared from the host; rather, viral genomes are maintained in cells in a latent phase. The virus can then reactivate, replicate again, and be infectious to others. In electron micrographs of HSV infection, it can be seen that the intact virus initially reacts with cell surface proteoglycans, then with specific receptors. This is later followed by viral capsids docking with nuclear pores. Afterward, the capsids go from being full to being "empty." Which of the following best fits these observations?

D) The viral envelope mediates entry into the cell, the capsid entry into the nuclear membrane, and the genome is all that enters the nucleus.

5) Which of the following accounts for someone who has had a herpesvirus-mediated cold sore or genital sore getting flare-ups for the rest of his or her life?

D) copies of the herpesvirus genome permanently maintained in host nuclei

18) What are prions?

D) misfolded versions of normal brain protein

44) Emerging viruses arise by

D) mutation of existing viruses, the spread of existing viruses to new host species, and the spread of existing viruses more widely within their host species.

22) Which of the following is the most probable fate of a newly emerging virus that causes high mortality in its host?

E) The newly emerging virus will die out rather quickly or will mutate to be far less lethal.

10) Most molecular biologists think that viruses originated from fragments of cellular nucleic acid. Which of the following observations supports this theory?

E) Viral genomes are usually similar to the genome of the host cell.

24) Which of the three types of viruses shown above would you expect to include a capsid(s)?

E) all three

26) In the figure, when new viruses are being assembled (IV), what mediates the assembly?

E) nothing; they self-assemble

3) The host range of a virus is determined by

E) the proteins on its surface and that of the host.

29) Some viruses can be crystallized and their structures analyzed. One such virus is Desmodium, or yellow mottle virus, which infects beans. This is a member of the tymovirus group and has a single-stranded RNA genome of ~6,300 nucleotides. Its virion is 25—30 nm in diameter, and is made up of 180 copies of a single capsid protein that self-associate to form each capsomere, which has icosahedral symmetry with 20 facets. How many nucleotides of the genome would you expect to find in one capsid?

E) ~6,300