Chapter 7

what is the range for pipettes

-P1000: 200-1000ul -P200: 20-200 ul -P20: 2-20 ul

Have a look at the BSA scatter plot in the second tab. What is the range of absorbance values measured for the BSA standard

0.08-0.65

what was the original concentration in the BHL sample, if the dilution is 1:500 and the conentration

35 mg

In table 12.1 which of these spores are characteristic of Rhizopus

7-8

Which of the following statements about algae is false?

All are unicellular

The Bradford assay only works at low protein concentrations (from 0.05 to 0.5 mg/mL protein) How can we reduce the concentration of protein in our sample.

Dilute the sample

Bacterial predators such as Bdellovibrio are members of the betaproteobacteria.

False

The human body is typically free of archaeal species

False

Which of the following statements regarding fungi is false

Most fungi are pathogenic for humans

Seventeen patients in ten hospitals had cutaneous infections caused by Rhizopus. In all 17 patients, Elastoplast bandages were placed over sterile gauze pads to cover wounds. Fourteen of the patients had surgical wounds, two had venous line insertion sites, and one had a bite wound. Lesions present when the bandages were removed ranged from vesiculopustular eruptions to ulcerations and skin necrosis requiring debridement. Fungi are more likely than bacteria to contaminate bandages because

They can tolerate low-moisture conditions

Which of the following bacteria is gram-negative

Treponema

Why are only sterile pipeete tips used in the lab

avoid contamination of sample

What do you need to do next before pipetting a different sample

discard the tip

The nonsulfu photosynthetic bacteria use organic compounds as

electron donors to reduce CO2

Which of the following is the most effective control for malaria

eliminate anopheles

The insect vectors have six legs and include ticks, mosquitoes, and lice.

false

Which of the following arthropods does NOT transmit diseases by sucking blood from a human host?

houseflies

Burkolderia was reclassifed from the gammaproteobacteria to the betaproteobacteria because

its rRNA sequence is similar to that of neisseria

Mycoplasmas differ from other bacteria in that they

lack a cell wall

Actinomycetes differ from fungi in that actinomycetes

lack a membrane bounded nucleus

Giardia and Trichomonas are unusual eukaryotes because they

lack mitochondria

Which of the following statements regarding protozoa is falze

nearly all protozoa cause disease

Borrelia is classified as a spirochete because it

possesses an axial filament

Which one of the following bacteria does not belong with the others

salmonella

Which of the following bacteria are responsible for more infections and more different kinds of infections

streptococcus

The cell of plasmodial slime molds can grow to several centimeters in diameter because

they distribute nutrients by ctyoplasmic streaming

Why did you press the plunger all the way down to the second stop

to push out the remaining fluid from the tip