Chapter 9 Quiz

An advantage of synthetic DNA over genomic or cDNA is the ability to: Select one: a. Insert desired restriction sites into the DNA sequence. b. Isolate unknown genes. c. Make DNA from cellular RNA and the enzyme reverse transcriptase. d. Obtain genes that lack introns. e. Obtain genes that lack exons.

a. Insert desired restriction sites into the DNA sequence.

Pieces of DNA stored in yeast cells are called a: Select one: a. Library. b. Clone. c. Vector. d. Southern blot. e. PCR.

a. Library.

PCR can be used to identify an unknown bacterium because: Select one: a. The RNA primer is specific. b. DNA polymerase will replicate DNA. c. DNA can be electrophoresed. d. All cells have DNA. e. All cells have RNA.

a. The RNA primer is specific.

Bioinformatics is the use of computer technology to compare and analyze genome sequence.: Select one: a. True. b. False.

a. True.

Restriction enzymes are: Select one: a. Bacterial enzymes that splice DNA. b. Bacterial enzymes that destroy phage DNA. c. Animal enzymes that splice RNA. d. Viral enzymes that destroy host DNA.

b. Bacterial enzymes that destroy phage DNA.

A population of cells carrying a desired plasmid is called a: Select one: a. Library. b. Clone. c. Vector. d. Southern blot. e. PCR.

b. Clone.

The term biotechnology refers exclusively to the use of genetically engineered organisms for the production of desired products.: Select one: a. True. b. False.

b. False.

An advantage of cDNA over genomic DNA is that it: Select one: a. Lacks exons. b. Lacks introns. c. Contains selectable markers. d. Can form very large DNA segments. e. Is very easy to isolate.

b. Lacks introns.

Which of the following is NOT a desired characteristic of DNA vectors used in gene cloning procedures?: Select one: a. Self-replication. b. Large size. c. Has a selectable marker. d. Circular form of DNA or integrates into the host chromosome. e. May replicate in several species.

b. Large size.

The random shotgun method is used in: Select one: a. Amplification of unknown DNA. b. Transforming plant cells with recombinant DNA. c. Genome sequencing. d. RFLP analysis. e. Forensic microbiology.

c. Genome sequencing.

Gene silencing involves all of the following EXCEPT: Select one: a. Small interfering RNAs. b. Production of double stranded RNAs. c. Small interfering RNA binding to a gene promoter. d. Dicer. e. RNA-induced silencing complex.

c. Small interfering RNA binding to a gene promoter.

If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is: Select one: a. Transformation of E. coli with Ti plasmid. b. Splicing T DNA into a plasmid. c. Transformation of an animal cell. d. Inserting the Ti plasmid into Agrobacterium. e. Inserting the Ti plasmid into a plant cell.

d. Inserting the Ti plasmid into Agrobacterium.

The study of genetic material taken directly from the environment is: Select one: a. Bioinformatics. b. Proteomics. c. Reverse genetics. d. Forensic microbiology. e. Metagenomics.

e. Metagenomics.

Biotechnology involves the: Select one: a. Use of microorganisms to make desired products. b. Use of animal cells to make vaccines. c. Development of disease-resistant crop plants. d. Use of microorganisms to make desired products and the use of animal cells to make vaccines. e. Use of microorganisms to make desired products, the use of animal cells to make vaccines, and the development of disease-resistant crop plants.

e. Use of microorganisms to make desired products, the use of animal cells to make vaccines, and the development of disease-resistant crop plants.