Clin Chem CH 5 Review

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Reverse-phase liquid chromatography refers to:

A polar mobile phase and nonpolar stationary phase

What is beer's law?

A=Ebc

Which electrochemical assay measures current at fixed potential?

Amperometry

Which of the following techniques has the highest potential sensitivity?

Chemiluminescence

What is the purpose of the chopper in an atomic absorption spectrophotometry?

Correct for the amount of light emitted by the flame

Which of the following is false concerning the principles of point-of-care testing devices?

Devices do not require quality control testing

Which of the following refers to the movement of buffer ions and solvent relative to a fixed support?

Electroendosmosis

Which of the following is true concerning fluorometry?

Fluorescence is an inherently more sensitive technique than absorption

Which is most accurate concerning ISEs?

Gas specific membranes are necessary for oxygen and carbon dioxide electrodes

Which of the following light sources is used in atomic absorption spectrophotometry?

Hollow-cathode lamp

Which of the following is not an object of proteomics research?

Identify specific gene mutations

Which of the following regarding MS is false?

Mass spectrometers can be used to sequence DNA

Which of the following correctly ranks electromagnetic radiation from low energy to high energy?

Microwaves, infrared, visible, UV, X-rays, gamma, cosmic

Which of the following best describes the process of fluorescence?

Molecules emit a photon at lower energy when excited electrons return to the ground state

Which of the following is not an advantage of CE?

Multiple samples can be assayed simultaneously on one injection

Which is the most sensitive detector for spectrophotometry?

Photomultiplier

Which of the following procedures is not currently or routinely used for point-of-care testing devices?

Polymerase chain reaction

Which of the following is not necessary for obtaining the spectrum of a compound from 190 to 500 nm?

Tungsten light source

Tandem mass spectrometers:

are 2 mass spectrometers placed in series with each other

Stray light in a spectrophotometer places limits on:

upper range of linearity


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